Restriction map for pF1KB6489
##################################
# NO CUT ENZYMES (  157 enzymes) #
##################################

AatII       AccI        AccII       AcsI        AcyI        
AflII       AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanII       BbeI        BbrPI       BbvCI       
BbvI        BcgI        BclI        BfrI        BglI        
BlnI        BlpI        BmyI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsoBI       Bsp1286I    BspDI       BsrBI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FbaI        
Fnu4HI      FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        ItaI        KasI        Ksp632I     
KspI        MamI        MfeI        MluI        MluNI       
MscI        MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       PvuI        RsrII       
SacI        SacII       SalI        SapI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
SspI        SwaI        TaqI        TseI        Tsp509I     
Tth111I     TthHB8I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

Acc65I         12  GGTACC               G^GTACC
AccIII        827  TCCGGA               T^CCGGA
AclI          977  AACGTT               AA^CGTT
AlwI           70  GATCC                GGATC(4/5)
Asp718I        12  GGTACC               G^GTACC
AvaII         118  GGACC                G^G[AT]CC
BanI           12  GGTACC               G^G[CT][GA]CC
BbsI          472  GAAGAC               GAAGAC(2/6)
BcnI          528  CCGGG                CC^[GC]GG
BfaI          892  CTAG                 C^TAG
BglII         964  AGATCT               A^GATCT
BpmI          625  CTGGAG               CTGGAG(16/14)
BpuAI         472  GAAGAC               GAAGAC(2/6)
BsaI          570  GAGACC               GGTCTC(1/5)
BseAI         827  TCCGGA               T^CCGGA
BseRI          86  CTCCTC               GAGGAG(10/8)
BsmAI         570  GAGAC                GTCTC(1/5)
BsmFI         136  GTCCC                GGGAC(10/14)
BsmI          495  GAATGC               GAATGC(1/-1)
Bsp1407I      695  TGTACA               T^GTACA
BspEI         827  TCCGGA               T^CCGGA
BspHI         557  TCATGA               T^CATGA
BsrDI         853  GCAATG               GCAATG(2/0)
BsrGI         695  TGTACA               T^GTACA
EcoT14I        73  CCAAGG               C^C[AT][AT]GG
HinfI         772  GATTC                G^A[ACGT]TC
KpnI           12  GGTACC               GGTAC^C
MaeI          892  CTAG                 C^TAG
MroI          827  TCCGGA               T^CCGGA
MseI          434  TTAA                 T^TAA
MslI          558  CATGACAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        131  CAGCTG               C[AC]G^C[GT]G
NciI          528  CCGGG                CC^[GC]GG
PpuMI         117  AGGACCC              [GA]G^G[AT]CC[CT]
Psp1406I      977  AACGTT               AA^CGTT
PstI           35  CTGCAG               CTGCA^G
PvuII         131  CAGCTG               CAG^CTG
RcaI          557  TCATGA               T^CATGA
SbfI           34  CCTGCAGG             CCTGCA^GG
Sse8387I       34  CCTGCAGG             CCTGCA^GG
SspBI         695  TGTACA               T^GTACA
StuI           76  AGGCCT               AGG^CCT
StyI           73  CCAAGG               C^C[AT][AT]GG
TfiI          772  GATTC                G^A[AT]TC
Tru9I         434  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AciI          667  GCGG                 CCGC(-3/-1)
              691  GCGG                
AflIII        366  ACATGT               A^C[GA][CT]GT
              403  ACATGT              
BciVI         175  GGATAC               GTATCC(6/5)
              230  GTATCC              
BsaWI         242  TCCGGT               [AT]^CCGG[AT]
              827  TCCGGA              
BsgI          631  GTGCAG               GTGCAG(16/14)
              684  GTGCAG              
BspLU11I      366  ACATGT               A^CATGT
              403  ACATGT              
BspMI         586  ACCTGC               ACCTGC(4/8)
               37  GCAGGT              
BstXI         216  CCAGCCAGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              286  CCAATAACATGG        
BstYI          69  AGATCC               [GA]^GATC[CT]
              964  AGATCT              
DpnII          70  GATC                 ^GATC
              965  GATC                
DraII         117  AGGACCC              [GA]G^G[ACGT]CC[CT]
              514  AGGCCCT             
EcoO109I      117  AGGACCC              [GA]G^G[ACGT]CC[CT]
              514  AGGCCCT             
HphI           40  GGTGA                GGTGA(8/7)
              650  TCACC               
MaeII         461  ACGT                 A^CGT
              978  ACGT                
MboI           70  GATC                 ^GATC
              965  GATC                
MflI           69  AGATCC               [GA]^GATC[CT]
              964  AGATCT              
NdeII          70  GATC                 ^GATC
              965  GATC                
NspI          366  ACATGT               [GA]CATG^[CT]
              403  ACATGT              
PflMI         142  CCACCTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              220  CCAGTTGGTGG         
Sau3AI         70  GATC                 ^GATC
              965  GATC                
SfcI           35  CTGCAG               C^T[GA][CT]AG
              180  CTACAG              
Tsp45I        532  GTCAC                ^GT[GC]AC
              649  GTCAC               
TspRI         534  CACTG                CA[GC]TG[ACGT][ACGT]^
              634  CAGTG               
Van91I        142  CCACCTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              220  CCAGTTGGTGG         
XcmI          216  CCAGCCAGTTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              614  CCAGACAAGAGCTGG     
XhoII          69  AGATCC               [GA]^GATC[CT]
              964  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   30 enzymes) #
#########################################

AfaI        AluI        AlwNI       BsaJI       BsiYI       
BslI        BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        EcoRII      FokI        
HaeIII      HapII       HpaII       MaeIII      MboII       
MnlI        MspI        MvaI        MwoI        NlaIII      
NlaIV       RsaI        Sau96I      ScrFI       SfaNI       
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