Restriction map for pF1KB6489
 (Linear) (Six-base) MAPSORT of: KIBB6489.seq  Check: 8029  from: 1  to: 987



KIBB6489.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      28     595     987 
   Size:         28     567     392

  Fragments arranged by size: 

                 567     392      28
 
Acc65I G'GTAC_C

Cuts at:      0      12     987 
   Size:         12     975
 
AclI AA'CG_TT

Cuts at:      0     978     987 
   Size:        978       9
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     561     987 
   Size:        358     203     426

  Fragments arranged by size: 

                 426     358     203
 
AflIII A'CryG_T

Cuts at:      0     366     403     987 
   Size:        366      37     584

  Fragments arranged by size: 

                 584     366      37
 
AlwNI CAG_nnn'CTG

Cuts at:      0      25     775     865     987 
   Size:         25     750      90     122

  Fragments arranged by size: 

                 750     122      90      25
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     168     201     987 
   Size:        168      33     786

  Fragments arranged by size: 

                 786     168      33
 
BanI G'GyrC_C

Cuts at:      0      12     987 
   Size:         12     975
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     479     987 
   Size:        479     508
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     169     241     987 
   Size:        169      72     746

  Fragments arranged by size: 

                 746     169      72
 
BglII A'GATC_T

Cuts at:      0     964     987 
   Size:        964      23
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     191     987 
   Size:        191     796
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     646     987 
   Size:        646     341
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     564     987 
   Size:        564     423
 
BsaWI w'CCGG_w

Cuts at:      0     242     827     987 
   Size:        242     585     160

  Fragments arranged by size: 

                 585     242     160
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     619     649     987 
   Size:        619      30     338

  Fragments arranged by size: 

                 619     338      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      77     987 
   Size:         77     910
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     652     705     987 
   Size:        652      53     282

  Fragments arranged by size: 

                 652     282      53
 
BsmI GAATG_Cn'

Cuts at:      0     501     987 
   Size:        501     486
 
BspEI T'CCGG_A

Cuts at:      0     827     987 
   Size:        827     160
 
BspHI T'CATG_A

Cuts at:      0     557     987 
   Size:        557     430
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      28     595     987 
   Size:         28     567     392

  Fragments arranged by size: 

                 567     392      28
 
BsrDI GCAATG_nn'

Cuts at:      0     860     987 
   Size:        860     127
 
BsrGI T'GTAC_A

Cuts at:      0     695     987 
   Size:        695     292
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     223     293     987 
   Size:        223      70     694

  Fragments arranged by size: 

                 694     223      70
 
BstYI r'GATC_y

Cuts at:      0      69     964     987 
   Size:         69     895      23

  Fragments arranged by size: 

                 895      69      23
 
BtsI GCAGTG_nn'

Cuts at:      0     640     987 
   Size:        640     347
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     561     646     987 
   Size:        358     203      85     341

  Fragments arranged by size: 

                 358     341     203      85
 
EcoO109I rG'GnC_Cy

Cuts at:      0     118     515     987 
   Size:        118     397     472

  Fragments arranged by size: 

                 472     397     118
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     775     790     807     822     987 
   Size:        775      15      17      15     165

  Fragments arranged by size: 

                 775     165      17      15      15
 
KpnI G_GTAC'C

Cuts at:      0      16     987 
   Size:         16     971
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     335     833     987 
   Size:        335     498     154

  Fragments arranged by size: 

                 498     335     154
 
MslI CAynn'nnrTG

Cuts at:      0     562     987 
   Size:        562     425
 
MspA1I CmG'CkG

Cuts at:      0     133     987 
   Size:        133     854
 
NspI r_CATG'y

Cuts at:      0     370     407     987 
   Size:        370      37     580

  Fragments arranged by size: 

                 580     370      37
 
PciI A'CATG_T

Cuts at:      0     366     403     987 
   Size:        366      37     584

  Fragments arranged by size: 

                 584     366      37
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     148     226     987 
   Size:        148      78     761

  Fragments arranged by size: 

                 761     148      78
 
PpuMI rG'GwC_Cy

Cuts at:      0     118     987 
   Size:        118     869
 
PstI C_TGCA'G

Cuts at:      0      39     987 
   Size:         39     948
 
PvuII CAG'CTG

Cuts at:      0     133     987 
   Size:        133     854
 
SbfI CC_TGCA'GG

Cuts at:      0      39     987 
   Size:         39     948
 
SfcI C'TryA_G

Cuts at:      0      35     180     987 
   Size:         35     145     807

  Fragments arranged by size: 

                 807     145      35
 
StuI AGG'CCT

Cuts at:      0      78     987 
   Size:         78     909
 
StyI C'CwwG_G

Cuts at:      0      73     987 
   Size:         73     914
 
TatI w'GTAC_w

Cuts at:      0     695     987 
   Size:        695     292
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     619     651     987 
   Size:        619      32     336

  Fragments arranged by size: 

                 619     336      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     223     621     987 
   Size:        223     398     366

  Fragments arranged by size: 

                 398     366     223
 

 Enzymes that do cut:

     AarI   Acc65I     AclI     AcuI   AflIII    AlwNI     BaeI     BanI
     BbsI    BciVI    BglII     BmrI     BpmI     BsaI    BsaWI    BsaXI
    BseRI     BsgI     BsmI    BspEI    BspHI    BspMI    BsrDI    BsrGI
    BstXI    BstYI     BtsI  Eco57MI EcoO109I    Hin4I     KpnI     MmeI
     MslI   MspA1I     NspI     PciI    PflMI    PpuMI     PstI    PvuII
     SbfI     SfcI     StuI     StyI     TatI     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI     AfeI    AflII     AgeI     AhdI     AleI     AlfI
     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII    BamHI    BanII     BbeI    BbvCI     BcgI     BclI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     MfeI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI
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