Restriction map for pF1KE1081
##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

Acc65I      AccII       AccIII      AflIII      AgeI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AvrII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BspDI       BspEI       BspLU11I    
BsrBI       BsrFI       BssHII      BstAPI      BstEII      
BstPI       BstUI       CelII       Cfr10I      ClaI        
CpoI        DraI        EagI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoT22I     FauI        
FseI        HpaI        KasI        KpnI        KspI        
MluI        MroI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        PacI        PinAI       PmaCI       PmeI        
PmlI        PshBI       PvuI        RsrII       SacII       
SalI        SbfI        SfiI        SfoI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SwaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AclI         2770  AACGTT               AA^CGTT
AflII        1371  CTTAAG               C^TTAAG
AhdI         3627  GACTGTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       3373  GTGCAC               G^TGCAC
ApaLI        3373  GTGCAC               G^TGCAC
Asp700I      2876  GAATCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        3627  GACTGTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2630  GACGAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        4007  TGCGCA               TGC^GCA
BalI         4036  TGGCCA               TGG^CCA
BamHI        2704  GGATCC               G^GATCC
BanI         4048  GGTGCC               G^G[CT][GA]CC
BfrI         1371  CTTAAG               C^TTAAG
BstBI        1685  TTCGAA               TT^CGAA
DraIII       2853  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1905  GACGTCAGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         4280  CCATGG               C^C[GA][CT]GG
EaeI         4036  TGGCCA               [CT]^GGCC[GA]
Eam1105I     3627  GACTGTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI         831  GAATTC               G^AATTC
EcoRV         642  GATATC               GAT^ATC
FspI         4007  TGCGCA               TGC^GCA
HaeII         557  AGCGCC               [GA]GCGC^[CT]
HgaI         3412  GACGC                GACGC(5/10)
MfeI         2984  CAATTG               C^AATTG
MluNI        4036  TGGCCA               TGG^CCA
MscI         4036  TGGCCA               TGG^CCA
MspA1I       2750  CAGCTG               C[AC]G^C[GT]G
MunI         2984  CAATTG               C^AATTG
NcoI         4280  CCATGG               C^CATGG
NspI         3840  GCATGT               [GA]CATG^[CT]
NspV         1685  TTCGAA               TT^CGAA
PaeR7I        593  CTCGAG               C^TCGAG
PflFI        2630  GACGAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        4404  GGGACCC              [GA]G^G[AT]CC[CT]
PshAI        2677  GACCAAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     2770  AACGTT               AA^CGTT
PvuII        2750  CAGCTG               CAG^CTG
SacI          858  GAGCTC               GAGCT^C
SexAI        3395  ACCAGGT              A^CC[AT]GGT
SfuI         1685  TTCGAA               TT^CGAA
SmaI         4400  CCCGGG               CCC^GGG
SspI          220  AATATT               AAT^ATT
StuI         1697  AGGCCT               AGG^CCT
Tth111I      2630  GACGAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          726  TCTAGA               T^CTAGA
XcmI         3115  CCATTGCAGGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          593  CTCGAG               C^TCGAG
XmaCI        4400  CCCGGG               C^CCGGG
XmaI         4400  CCCGGG               C^CCGGG
XmnI         2876  GAATCATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AatII         298  GACGTC               GACGT^C
             1905  GACGTC              
AccI         1803  GTATAC               GT^[AC][GT]AC
             3365  GTATAC              
AciI         2163  GCGG                 CCGC(-3/-1)
             2166  GCGG                
AcyI          298  GACGTC               G[GA]^CG[CT]C
             1905  GACGTC              
AlwNI         556  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              980  CAGCATCTG           
AspHI         858  GAGCTC               G[AT]GC[AT]^C
             3373  GTGCAC              
BclI          449  TGATCA               T^GATCA
             3723  TGATCA              
BsaBI         902  GATGCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1462  GATCTTGATC          
BsaHI         298  GACGTC               G[GA]^CG[CT]C
             1905  GACGTC              
BsaI         1032  GAGACC               GGTCTC(1/5)
             1446  GAGACC              
BsgI         1630  CTGCAC               GTGCAG(16/14)
             1349  GTGCAG              
BsiHKAI       858  GAGCTC               G[AT]GC[AT]^C
             3373  GTGCAC              
BspHI          71  TCATGA               T^CATGA
             3380  TCATGA              
BspMI        1304  ACCTGC               ACCTGC(4/8)
             2944  ACCTGC              
BsrDI         752  CATTGC               GCAATG(2/0)
             3116  CATTGC              
BssSI        1512  CACGAG               CACGAG(-5/-1)
             3674  CTCGTG              
Bst1107I     1803  GTATAC               GTA^TAC
             3365  GTATAC              
BstZ17I      1803  GTATAC               GTA^TAC
             3365  GTATAC              
DraII        4404  GGGACCC              [GA]G^G[ACGT]CC[CT]
             4464  AGGCCCC             
EcoO109I     4404  GGGACCC              [GA]G^G[ACGT]CC[CT]
             4464  AGGCCCC             
FbaI          449  TGATCA               T^GATCA
             3723  TGATCA              
Hin1I         298  GACGTC               G[GA]^CG[CT]C
             1905  GACGTC              
MamI          902  GATGCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1462  GATCTTGATC          
PflMI        4028  CCAGGAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4039  CCATTCTTTGG         
RcaI           71  TCATGA               T^CATGA
             3380  TCATGA              
ScaI         2039  AGTACT               AGT^ACT
             4096  AGTACT              
Van91I       4028  CCAGGAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4039  CCATTCTTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        AocI        
ApoI        AvaI        AvaII       BanII       BbsI        
BbvI        BcnI        BfaI        BglII       BmyI        
BpmI        BpuAI       BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BsrGI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EarI        Eco81I      EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HincII      HindII      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PstI        RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspBI       
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
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