Restriction map for pF1KE1081
 (Linear) (Six-base) MAPSORT of: KIEE1081.seq  Check: 5836  from: 1  to: 4479



KIEE1081.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1313    4479 
   Size:       1313    3166
 
AatII G_ACGT'C

Cuts at:      0     302    1909    4479 
   Size:        302    1607    2570

  Fragments arranged by size: 

                2570    1607     302
 
AccI GT'mk_AC

Cuts at:      0    1804    3366    4479 
   Size:       1804    1562    1113

  Fragments arranged by size: 

                1804    1562    1113
 
AclI AA'CG_TT

Cuts at:      0    2771    4479 
   Size:       2771    1708
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     488     689    3947    4479 
   Size:        488     201    3258     532

  Fragments arranged by size: 

                3258     532     488     201
 
AflII C'TTAA_G

Cuts at:      0    1371    4479 
   Size:       1371    3108
 
AhdI GACnn_n'nnGTC

Cuts at:      0    3632    4479 
   Size:       3632     847
 
AleI CACnn'nnGTG

Cuts at:      0    1798    4479 
   Size:       1798    2681
 
AlwNI CAG_nnn'CTG

Cuts at:      0     561     985    4479 
   Size:        561     424    3494

  Fragments arranged by size: 

                3494     561     424
 
ApaLI G'TGCA_C

Cuts at:      0    3373    4479 
   Size:       3373    1106
 
ApoI r'AATT_y

Cuts at:      0      76     695     831    1110    1165    1560    1658    1725 
   Size:         76     619     136     279      55     395      98      67

Cuts at:   1725    2782    3056    3226    3314    3433    3892    4479 
   Size:       1057     274     170      88     119     459     587

  Fragments arranged by size: 

                1057     619     587     459     395     279     274     170
                 136     119      98      88      76      67      55
 
AvaI C'yCGr_G

Cuts at:      0     593    4291    4400    4479 
   Size:        593    3698     109      79

  Fragments arranged by size: 

                3698     593     109      79
 
BamHI G'GATC_C

Cuts at:      0    2704    4479 
   Size:       2704    1775
 
BanI G'GyrC_C

Cuts at:      0    4048    4479 
   Size:       4048     431
 
BanII G_rGCy'C

Cuts at:      0     843     862    3155    4479 
   Size:        843      19    2293    1324

  Fragments arranged by size: 

                2293    1324     843      19
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     878    1886    3096    3529    4479 
   Size:        878    1008    1210     433     950

  Fragments arranged by size: 

                1210    1008     950     878     433
 
BclI T'GATC_A

Cuts at:      0     449    3723    4479 
   Size:        449    3274     756

  Fragments arranged by size: 

                3274     756     449
 
BglII A'GATC_T

Cuts at:      0     267    2593    3759    4479 
   Size:        267    2326    1166     720

  Fragments arranged by size: 

                2326    1166     720     267
 
Bme1580I G_kGCm'C

Cuts at:      0    3377    4479 
   Size:       3377    1102
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1792    4479 
   Size:       1792    2687
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     626    1935    2847    4479 
   Size:        626    1309     912    1632

  Fragments arranged by size: 

                1632    1309     912     626
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     292     876     903    1872    3815    4361    4479 
   Size:         79     213     584      27     969    1943     546     118

  Fragments arranged by size: 

                1943     969     584     546     213     118      79      27
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     562    3041    4479 
   Size:        562    2479    1438

  Fragments arranged by size: 

                2479    1438     562
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     596    3288    4479 
   Size:        154     442    2692    1191

  Fragments arranged by size: 

                2692    1191     442     154
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1026    1440    4479 
   Size:       1026     414    3039

  Fragments arranged by size: 

                3039    1026     414
 
BsaBI GATnn'nnATC

Cuts at:      0     906    1466    4479 
   Size:        906     560    3013

  Fragments arranged by size: 

                3013     906     560
 
BsaHI Gr'CG_yC

Cuts at:      0     299    1906    4479 
   Size:        299    1607    2573

  Fragments arranged by size: 

                2573    1607     299
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1060    1090    4479 
   Size:       1060      30    3389

  Fragments arranged by size: 

                3389    1060      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     599    1613    1968    2291    3175    4479 
   Size:        599    1014     355     323     884    1304

  Fragments arranged by size: 

                1304    1014     884     599     355     323
 
BseYI C'CCAG_C

Cuts at:      0    1236    4479 
   Size:       1236    3243
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1370    1615    4479 
   Size:       1370     245    2864

  Fragments arranged by size: 

                2864    1370     245
 
BsiHKAI G_wGCw'C

Cuts at:      0     862    3377    4479 
   Size:        862    2515    1102

  Fragments arranged by size: 

                2515    1102     862
 
BsmI GAATG_Cn'

Cuts at:      0    2385    3213    4242    4479 
   Size:       2385     828    1029     237

  Fragments arranged by size: 

                2385    1029     828     237
 
Bsp1286I G_dGCh'C

Cuts at:      0     843     862    3155    3377    4479 
   Size:        843      19    2293     222    1102

  Fragments arranged by size: 

                2293    1102     843     222      19
 
BspHI T'CATG_A

Cuts at:      0      71    3380    4479 
   Size:         71    3309    1099

  Fragments arranged by size: 

                3309    1099      71
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1313    2953    4479 
   Size:       1313    1640    1526

  Fragments arranged by size: 

                1640    1526    1313
 
BsrDI GCAATG_nn'

Cuts at:      0     751    3115    4479 
   Size:        751    2364    1364

  Fragments arranged by size: 

                2364    1364     751
 
BsrGI T'GTAC_A

Cuts at:      0    2491    4116    4178    4479 
   Size:       2491    1625      62     301

  Fragments arranged by size: 

                2491    1625     301      62
 
BssSI C'ACGA_G

Cuts at:      0    1512    3674    4479 
   Size:       1512    2162     805

  Fragments arranged by size: 

                2162    1512     805
 
BstBI TT'CG_AA

Cuts at:      0    1686    4479 
   Size:       1686    2793
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      35     835    2352    3403    3972    4422    4479 
   Size:         35     800    1517    1051     569     450      57

  Fragments arranged by size: 

                1517    1051     800     569     450      57      35
 
BstYI r'GATC_y

Cuts at:      0     267     889    1891    2593    2704    2776    3759    4479 
   Size:        267     622    1002     702     111      72     983     720

  Fragments arranged by size: 

                1002     983     720     702     622     267     111      72
            
 
BstZ17I GTA'TAC

Cuts at:      0    1805    3367    4479 
   Size:       1805    1562    1112

  Fragments arranged by size: 

                1805    1562    1112
 
Bsu36I CC'TnA_GG

Cuts at:      0    1451    2423    3187    4479 
   Size:       1451     972     764    1292

  Fragments arranged by size: 

                1451    1292     972     764
 
BtgI C'CryG_G

Cuts at:      0    4280    4479 
   Size:       4280     199
 
BtsI GCAGTG_nn'

Cuts at:      0    4381    4479 
   Size:       4381      98
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1580    1615    4479 
   Size:       1580      35    2864

  Fragments arranged by size: 

                2864    1580      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    2858    4479 
   Size:       2858    1621
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1911    4479 
   Size:       1911    2568
 
EaeI y'GGCC_r

Cuts at:      0    4036    4479 
   Size:       4036     443
 
EarI CTCTTCn'nnn_

Cuts at:      0     323     365     464    1007    2351    2390    2576    3160 
   Size:        323      42      99     543    1344      39     186     584

Cuts at:   3160    3332    3923    4442    4479 
   Size:        172     591     519      37

  Fragments arranged by size: 

                1344     591     584     543     519     323     186     172
                  99      42      39      37
 
EcoICRI GAG'CTC

Cuts at:      0     860    4479 
   Size:        860    3619
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     292     488     689     876     903    1872    3815 
   Size:         79     213     196     201     187      27     969    1943

Cuts at:   3815    3947    4361    4479 
   Size:        132     414     118

  Fragments arranged by size: 

                1943     969     414     213     201     196     187     132
                 118      79      27
 
EcoO109I rG'GnC_Cy

Cuts at:      0    4405    4465    4479 
   Size:       4405      60      14

  Fragments arranged by size: 

                4405      60      14
 
EcoRI G'AATT_C

Cuts at:      0     831    4479 
   Size:        831    3648
 
EcoRV GAT'ATC

Cuts at:      0     644    4479 
   Size:        644    3835
 
FspI TGC'GCA

Cuts at:      0    4009    4479 
   Size:       4009     470
 
FspAI rTGC'GCAy

Cuts at:      0    4009    4479 
   Size:       4009     470
 
HaeII r_GCGC'y

Cuts at:      0     561    4479 
   Size:        561    3918
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     259     291     844     876     982    1014    1129    1161 
   Size:        259      32     553      32     106      32     115      32

Cuts at:   1161    1177    1209    1846    1878    2008    2040    3618    3650 
   Size:         16      32     637      32     130      32    1578      32

Cuts at:   3650    3708    3740    4479 
   Size:         58      32     739

  Fragments arranged by size: 

                1578     739     637     553     259     130     115     106
                  58      32      32      32      32      32      32      32
                  32      32      16
 
HincII GTy'rAC

Cuts at:      0    2303    2589    2973    4479 
   Size:       2303     286     384    1506

  Fragments arranged by size: 

                2303    1506     384     286
 
HindIII A'AGCT_T

Cuts at:      0     173     541     801    4479 
   Size:        173     368     260    3678

  Fragments arranged by size: 

                3678     368     260     173
 
MfeI C'AATT_G

Cuts at:      0    2984    4479 
   Size:       2984    1495
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1642    2710    2754    4479 
   Size:       1642    1068      44    1725

  Fragments arranged by size: 

                1725    1642    1068      44
 
MscI TGG'CCA

Cuts at:      0    4038    4479 
   Size:       4038     441
 
MslI CAynn'nnrTG

Cuts at:      0    1798    3463    4124    4479 
   Size:       1798    1665     661     355

  Fragments arranged by size: 

                1798    1665     661     355
 
MspA1I CmG'CkG

Cuts at:      0    2752    4479 
   Size:       2752    1727
 
NcoI C'CATG_G

Cuts at:      0    4280    4479 
   Size:       4280     199
 
NspI r_CATG'y

Cuts at:      0    3844    4479 
   Size:       3844     635
 
PasI CC'CwG_GG

Cuts at:      0    2235    4479 
   Size:       2235    2244
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    4034    4045    4479 
   Size:       4034      11     434

  Fragments arranged by size: 

                4034     434      11
 
PfoI T'CCnGG_A

Cuts at:      0    1850    1900    3217    4479 
   Size:       1850      50    1317    1262

  Fragments arranged by size: 

                1850    1317    1262      50
 
PpuMI rG'GwC_Cy

Cuts at:      0    4405    4479 
   Size:       4405      74
 
PshAI GACnn'nnGTC

Cuts at:      0    2681    4479 
   Size:       2681    1798
 
PsiI TTA'TAA

Cuts at:      0    2959    4479 
   Size:       2959    1520
 
PstI C_TGCA'G

Cuts at:      0    2337    2420    2918    2970    3050    4479 
   Size:       2337      83     498      52      80    1429

  Fragments arranged by size: 

                2337    1429     498      83      80      52
 
PvuII CAG'CTG

Cuts at:      0    2752    4479 
   Size:       2752    1727
 
SacI G_AGCT'C

Cuts at:      0     862    4479 
   Size:        862    3617
 
SanDI GG'GwC_CC

Cuts at:      0    4405    4479 
   Size:       4405      74
 
SapI GCTCTTCn'nnn_

Cuts at:      0     323    2390    3160    3923    4479 
   Size:        323    2067     770     763     556

  Fragments arranged by size: 

                2067     770     763     556     323
 
ScaI AGT'ACT

Cuts at:      0    2041    4098    4479 
   Size:       2041    2057     381

  Fragments arranged by size: 

                2057    2041     381
 
SexAI A'CCwGG_T

Cuts at:      0    3395    4479 
   Size:       3395    1084
 
SfcI C'TryA_G

Cuts at:      0    1667    2262    2333    2416    2914    2966    3046    4479 
   Size:       1667     595      71      83     498      52      80    1433

  Fragments arranged by size: 

                1667    1433     595     498      83      80      71      52
            
 
SmaI CCC'GGG

Cuts at:      0    4402    4479 
   Size:       4402      77
 
SmlI C'TyrA_G

Cuts at:      0     133     593     611    1371    3303    4479 
   Size:        133     460      18     760    1932    1176

  Fragments arranged by size: 

                1932    1176     760     460     133      18
 
SspI AAT'ATT

Cuts at:      0     222    4479 
   Size:        222    4257
 
StuI AGG'CCT

Cuts at:      0    1699    4479 
   Size:       1699    2780
 
StyI C'CwwG_G

Cuts at:      0    1547    2679    4280    4479 
   Size:       1547    1132    1601     199

  Fragments arranged by size: 

                1601    1547    1132     199
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     569    4470    4479 
   Size:        569    3901       9

  Fragments arranged by size: 

                3901     569       9
 
TatI w'GTAC_w

Cuts at:      0    1486    2039    2391    2491    4096    4116    4178    4479 
   Size:       1486     553     352     100    1605      20      62     301

  Fragments arranged by size: 

                1605    1486     553     352     301     100      62      20
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     948     980    1060    1092    3492    3524    4479 
   Size:        948      32      80      32    2400      32     955

  Fragments arranged by size: 

                2400     955     948      80      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2633    4479 
   Size:       2633    1846
 
XbaI T'CTAG_A

Cuts at:      0     726    4479 
   Size:        726    3753
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    3122    4479 
   Size:       3122    1357
 
XhoI C'TCGA_G

Cuts at:      0     593    4479 
   Size:        593    3886
 
XmaI C'CCGG_G

Cuts at:      0    4400    4479 
   Size:       4400      79
 
XmnI GAAnn'nnTTC

Cuts at:      0    2880    4479 
   Size:       2880    1599
 
ZraI GAC'GTC

Cuts at:      0     300    1907    4479 
   Size:        300    1607    2572

  Fragments arranged by size: 

                2572    1607     300
 

 Enzymes that do cut:

     AarI    AatII     AccI     AclI     AcuI    AflII     AhdI     AleI
    AlwNI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbsI
     BclI    BglII Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrGI    BssSI    BstBI
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI    CspCI   DraIII
     DrdI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     MfeI     MmeI
     MscI     MslI   MspA1I     NcoI     NspI     PasI    PflMI     PfoI
    PpuMI    PshAI     PsiI     PstI    PvuII     SacI    SanDI     SapI
     ScaI    SexAI     SfcI     SmaI     SmlI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AfeI   AflIII     AgeI     AlfI     AloI     ApaI     AscI
     AseI    AsiSI    AvrII     BaeI     BbeI    BbvCI     BcgI    BciVI
    BfrBI     BglI     BlpI    BmgBI     BmtI    BsaAI    BsaWI    BsiEI
    BsiWI    BsmBI    BspEI    BsrBI    BsrFI   BssHII   BstAPI   BstEII
    BtgZI     ClaI     DraI     EagI     EciI    EcoNI     FalI     FseI
     HpaI     KasI     KpnI     MluI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI
     PpiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
     SbfI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SwaI
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