Restriction map for pF1KB6375
 (Linear) (Six-base) MAPSORT of: KIBB6375.seq  Check: 2752  from: 1  to: 810



KIBB6375.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     197     810 
   Size:        197     613
 
AflIII A'CryG_T

Cuts at:      0     402     810 
   Size:        402     408
 
ApaI G_GGCC'C

Cuts at:      0     523     810 
   Size:        523     287
 
ApoI r'AATT_y

Cuts at:      0     484     810 
   Size:        484     326
 
BanII G_rGCy'C

Cuts at:      0     140     240     523     810 
   Size:        140     100     283     287

  Fragments arranged by size: 

                 287     283     140     100
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     320     479     810 
   Size:        320     159     331

  Fragments arranged by size: 

                 331     320     159
 
BbvCI CC'TCA_GC

Cuts at:      0      80     810 
   Size:         80     730
 
BglII A'GATC_T

Cuts at:      0     179     351     810 
   Size:        179     172     459

  Fragments arranged by size: 

                 459     179     172
 
BlpI GC'TnA_GC

Cuts at:      0     448     810 
   Size:        448     362
 
Bme1580I G_kGCm'C

Cuts at:      0     523     810 
   Size:        523     287
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     778     810 
   Size:        778      32
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403     810 
   Size:        403     407
 
Bpu10I CC'TnA_GC

Cuts at:      0      80     810 
   Size:         80     730
 
BsaAI yAC'GTr

Cuts at:      0     405     810 
   Size:        405     405
 
BsaBI GATnn'nnATC

Cuts at:      0      50     810 
   Size:         50     760
 
BsaHI Gr'CG_yC

Cuts at:      0     654     810 
   Size:        654     156
 
BseYI C'CCAG_C

Cuts at:      0     477     810 
   Size:        477     333
 
BsiHKAI G_wGCw'C

Cuts at:      0     449     810 
   Size:        449     361
 
BsmI GAATG_Cn'

Cuts at:      0     224     270     810 
   Size:        224      46     540

  Fragments arranged by size: 

                 540     224      46
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     739     810 
   Size:        739      71
 
Bsp1286I G_dGCh'C

Cuts at:      0     140     240     449     523     810 
   Size:        140     100     209      74     287

  Fragments arranged by size: 

                 287     209     140     100      74
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     197     810 
   Size:        197     613
 
BsrDI GCAATG_nn'

Cuts at:      0     425     810 
   Size:        425     385
 
BssSI C'ACGA_G

Cuts at:      0     250     810 
   Size:        250     560
 
BstBI TT'CG_AA

Cuts at:      0     216     729     810 
   Size:        216     513      81

  Fragments arranged by size: 

                 513     216      81
 
BstEII G'GTnAC_C

Cuts at:      0     585     810 
   Size:        585     225
 
BstYI r'GATC_y

Cuts at:      0     179     351     810 
   Size:        179     172     459

  Fragments arranged by size: 

                 459     179     172
 
Bsu36I CC'TnA_GG

Cuts at:      0     389     810 
   Size:        389     421
 
BtgI C'CryG_G

Cuts at:      0     716     810 
   Size:        716      94
 
DraI TTT'AAA

Cuts at:      0     489     810 
   Size:        489     321
 
EarI CTCTTCn'nnn_

Cuts at:      0     737     745     810 
   Size:        737       8      65

  Fragments arranged by size: 

                 737      65       8
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403     810 
   Size:        403     407
 
EcoO109I rG'GnC_Cy

Cuts at:      0     123     511     520     609     780     810 
   Size:        123     388       9      89     171      30

  Fragments arranged by size: 

                 388     171     123      89      30       9
 
HincII GTy'rAC

Cuts at:      0     708     810 
   Size:        708     102
 
MfeI C'AATT_G

Cuts at:      0     525     810 
   Size:        525     285
 
MslI CAynn'nnrTG

Cuts at:      0     567     810 
   Size:        567     243
 
MspA1I CmG'CkG

Cuts at:      0      66     681     759     810 
   Size:         66     615      78      51

  Fragments arranged by size: 

                 615      78      66      51
 
NcoI C'CATG_G

Cuts at:      0     716     810 
   Size:        716      94
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     355     810 
   Size:        355     455
 
PmlI CAC'GTG

Cuts at:      0     405     810 
   Size:        405     405
 
PpuMI rG'GwC_Cy

Cuts at:      0     123     511     780     810 
   Size:        123     388     269      30

  Fragments arranged by size: 

                 388     269     123      30
 
PspOMI G'GGCC_C

Cuts at:      0     519     810 
   Size:        519     291
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     276     308     810 
   Size:        276      32     502

  Fragments arranged by size: 

                 502     276      32
 
PstI C_TGCA'G

Cuts at:      0     194     283     617     810 
   Size:        194      89     334     193

  Fragments arranged by size: 

                 334     194     193      89
 
PvuII CAG'CTG

Cuts at:      0      66     681     759     810 
   Size:         66     615      78      51

  Fragments arranged by size: 

                 615      78      66      51
 
SfcI C'TryA_G

Cuts at:      0      41     190     279     613     810 
   Size:         41     149      89     334     197

  Fragments arranged by size: 

                 334     197     149      89      41
 
SmlI C'TyrA_G

Cuts at:      0      10     810 
   Size:         10     800
 
StyI C'CwwG_G

Cuts at:      0     515     716     810 
   Size:        515     201      94

  Fragments arranged by size: 

                 515     201      94
 
SwaI ATTT'AAAT

Cuts at:      0     489     810 
   Size:        489     321
 
TatI w'GTAC_w

Cuts at:      0     167     302     810 
   Size:        167     135     508

  Fragments arranged by size: 

                 508     167     135
 
XmnI GAAnn'nnTTC

Cuts at:      0     317     810 
   Size:        317     493
 

 Enzymes that do cut:

     AarI   AflIII     ApaI     ApoI    BanII     BbsI    BbvCI    BglII
     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BsaAI    BsaBI    BsaHI
    BseYI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrDI    BssSI
    BstBI   BstEII    BstYI   Bsu36I     BtgI     DraI     EarI  Eco57MI
 EcoO109I   HincII     MfeI     MslI   MspA1I     NcoI    PflMI     PmlI
    PpuMI   PspOMI     PsrI     PstI    PvuII     SfcI     SmlI     StyI
     SwaI     TatI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI
    BciVI     BclI    BfrBI     BglI    BmgBI     BmtI     BplI    BpuEI
     BsaI    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI    BspEI
    BspHI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI    BstXI  BstZ17I
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MluI
     MmeI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PpiI    PshAI     PsiI    PspXI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI
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