Restriction map for pF1KE0535
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AccIII      AclI        AcsI        AflII       AflIII      
AgeI        AhdI        AlwNI       AocI        Aor51HI     
ApoI        AscI        AseI        AsnI        Asp700I     
AspEI       AvrII       BalI        BbrPI       BbsI        
BcgI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BseRI       BsiEI       BsiWI       BsmAI       BsmBI       
BsmI        BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HincII      HindII      HindIII     HpaI        KspI        
MaeI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MunI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfuI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        Tru9I       XbaI        XcmI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AatII           6  GACGTC               GACGT^C
Acc65I        236  GGTACC               G^GTACC
AccI          415  GTCTAC               GT^[AC][GT]AC
Alw44I        669  GTGCAC               G^TGCAC
ApaI          657  GGGCCC               GGGCC^C
ApaLI         669  GTGCAC               G^TGCAC
Asp718I       236  GGTACC               G^GTACC
AspI          816  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          734  CCCGGG               C^[CT]CG[GA]G
AviII         404  TGCGCA               TGC^GCA
BamHI         578  GGATCC               G^GATCC
BbeI          830  GGCGCC               GGCGC^C
BbvCI         492  CCTCAGC              CCTCAGC(-5/-2)
BglI          393  GCCACACTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsoBI         734  CCCGGG               C^[CT]CG[GA]G
BsrBI         907  GAGCGG               CCGCTC(-3/-3)
BstYI         578  GGATCC               [GA]^GATC[CT]
DraIII        550  CACAGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI          892  CTCTTC               CTCTTC(1/4)
EcoNI         375  CCTCAATCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI          404  TGCGCA               TGC^GCA
HaeII         830  GGCGCC               [GA]GCGC^[CT]
KasI          830  GGCGCC               G^GCGCC
KpnI          236  GGTACC               GGTAC^C
Ksp632I       892  CTCTTC               CTCTTC(1/4)
MflI          578  GGATCC               [GA]^GATC[CT]
NarI          830  GGCGCC               GG^CGCC
PflFI         816  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SapI          891  GCTCTTC              GCTCTTC(1/4)
SfoI          830  GGCGCC               GGC^GCC
SgrAI         826  CACCGGCG             C[GA]^CCGG[CT]G
SmaI          734  CCCGGG               CCC^GGG
TaqI         1151  TCGA                 T^CGA
TfiI          291  GATTC                G^A[AT]TC
Tsp509I      1171  AATT                 ^AATT
Tth111I       816  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      1151  TCGA                 T^CGA
XhoII         578  GGATCC               [GA]^GATC[CT]
XmaCI         734  CCCGGG               C^CCGGG
XmaI          734  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

BpmI          271  CTGGAG               CTGGAG(16/14)
              643  CTGGAG              
Bsp1407I      329  TGTACA               T^GTACA
              425  TGTACA              
BsrFI         239  ACCGGC               [GA]^CCGG[CT]
              827  ACCGGC              
BsrGI         329  TGTACA               T^GTACA
              425  TGTACA              
BstEII        630  GGTCACC              G^GT[ACGT]ACC
              729  GGTCACC             
BstF5I         70  CATCC                GGATG(2/0)
              570  GGATG               
BstPI         630  GGTCACC              G^GT[ACGT]ACC
              729  GGTCACC             
BstXI         203  CCATTCTGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              968  CCACCAACGTGG        
Cfr10I        239  ACCGGC               [GA]^CCGG[CT]
              827  ACCGGC              
DdeI           91  CTGAG                C^T[ACGT]AG
              493  CTCAG               
DsaI           11  CCGTGG               C^C[GA][CT]GG
               52  CCGTGG              
EcoO65I       630  GGTCACC              G^GT[ACGT]ACC
              729  GGTCACC             
EcoT14I      1024  CCAAGG               C^C[AT][AT]GG
             1160  CCAAGG              
FokI           70  CATCC                GGATG(9/13)
              570  GGATG               
PflMI         565  CCATCGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              806  CCACCGACTGG         
PleI          709  GACTC                GAGTC(4/5)
              646  GAGTC               
SacI          748  GAGCTC               GAGCT^C
              889  GAGCTC              
SfcI          103  CTACAG               C^T[GA][CT]AG
              417  CTACAG              
SspBI         329  TGTACA               T^GTACA
              425  TGTACA              
StyI         1024  CCAAGG               C^C[AT][AT]GG
             1160  CCAAGG              
Van91I        565  CCATCGGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              806  CCACCGACTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AspHI       AvaII       BanI        BanII       
BbvI        BcnI        BmyI        BsaHI       BsaJI       
BsgI        BsiHKAI     BsiYI       BslI        BsmFI       
Bsp1286I    BspMI       BsrI        BssKI       BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DpnII       
DraII       EcoO109I    EcoRII      FauI        Fnu4HI      
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MnlI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PpuMI       PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       TseI        Tsp45I      TspRI       
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