Restriction map for pF1KB8896
##################################
# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BfrI        BglI        BglII       BlnI        BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsgI        BsiEI       BsiWI       BsmAI       
BsmBI       BsmI        BsoBI       BspDI       BspEI       
BspHI       BspMI       BsrBI       BsrDI       BsrFI       
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FseI        FspI        HaeII       
HaeIII      HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KspI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
SexAI       SfaNI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SphI        
Sse8387I    SspI        StuI        SwaI        Tru9I       
Tsp45I      Tth111I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I        580  GGTACC               G^GTACC
AcsI          373  GAATTT               [GA]^AATT[CT]
AflIII        530  ACATGT               A^C[GA][CT]GT
Alw44I        331  GTGCAC               G^TGCAC
AlwNI         613  CAGGCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI         331  GTGCAC               G^TGCAC
ApoI          373  GAATTT               [GA]^AATT[CT]
Asp718I       580  GGTACC               G^GTACC
AspHI         331  GTGCAC               G[AT]GC[AT]^C
BanI          580  GGTACC               G^G[CT][GA]CC
BbsI          284  GAAGAC               GAAGAC(2/6)
BcnI          321  CCCGG                CC^[GC]GG
BlpI          436  GCTCAGC              GC^T[ACGT]AGC
BmyI          331  GTGCAC               G[AGT]GC[ACT]^C
Bpu1102I      436  GCTCAGC              GC^T[ACGT]AGC
BpuAI         284  GAAGAC               GAAGAC(2/6)
BsiHKAI       331  GTGCAC               G[AT]GC[AT]^C
Bsp1286I      331  GTGCAC               G[AGT]GC[ACT]^C
Bsp1407I      527  TGTACA               T^GTACA
BspLU11I      530  ACATGT               A^CATGT
BsrGI         527  TGTACA               T^GTACA
BssSI         188  CTCGTG               CACGAG(-5/-1)
BstF5I        299  CATCC                GGATG(2/0)
BstYI          20  GGATCT               [GA]^GATC[CT]
Cac8I         612  GCAGGC               GC[ACGT]^[ACGT]GC
CelII         436  GCTCAGC              GC^T[ACGT]AGC
EarI          202  CTCTTC               CTCTTC(1/4)
EcoNI         453  CCTGGGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI          299  CATCC                GGATG(9/13)
HapII         322  CCGG                 C^CGG
HpaII         322  CCGG                 C^CGG
KpnI          580  GGTACC               GGTAC^C
Ksp632I       202  CTCTTC               CTCTTC(1/4)
MflI           20  GGATCT               [GA]^GATC[CT]
MspI          322  CCGG                 C^CGG
MwoI          132  GCTGGAGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI          321  CCCGG                CC^[GC]GG
NspI          530  ACATGT               [GA]CATG^[CT]
SpeI          240  ACTAGT               A^CTAGT
SspBI         527  TGTACA               T^GTACA
Tsp509I       374  AATT                 ^AATT
XcmI          443  CCAAGATTGTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII          20  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AciI           10  CCGC                 CCGC(-3/-1)
              130  CCGC                
AfaI          528  GTAC                 GT^AC
              581  GTAC                
AlwI           20  GGATC                GGATC(4/5)
              380  GGATC               
BbvI           12  GCTGC                GCAGC(8/12)
              546  GCTGC               
BclI          108  TGATCA               T^GATCA
              122  TGATCA              
BfaI           92  CTAG                 C^TAG
              241  CTAG                
BpmI          326  CTCCAG               CTGGAG(16/14)
              133  CTGGAG              
BsmFI         388  GGGAC                GGGAC(10/14)
              536  GGGAC               
BstXI          35  CCAGCCTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              596  CCAATATCCTGG        
DdeI           67  CTCAG                C^T[ACGT]AG
              437  CTCAG               
EcoT14I       219  CCAAGG               C^C[AT][AT]GG
              656  CCTTGG              
FbaI          108  TGATCA               T^GATCA
              122  TGATCA              
HphI          399  GGTGA                GGTGA(8/7)
               32  TCACC               
MaeI           92  CTAG                 C^TAG
              241  CTAG                
MboII         284  GAAGA                GAAGA(8/7)
              203  TCTTC               
MslI          334  CACATCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              336  CATCTGTGTG          
MspA1I         10  CCGCTG               C[AC]G^C[GT]G
              130  CCGCTG              
NlaIII        112  CATG                 CATG^
              531  CATG                
PleI          538  GACTC                GAGTC(4/5)
              352  GAGTC               
RsaI          528  GTAC                 GT^AC
              581  GTAC                
StyI          219  CCAAGG               C^C[AT][AT]GG
              656  CCTTGG              
TaqI          168  TCGA                 T^CGA
              311  TCGA                
TfiI           97  GAATC                G^A[AT]TC
              469  GATTC               
TseI           12  GCTGC                G^C[AT]GC
              546  GCTGC               
TspRI          69  CAGTG                CA[GC]TG[ACGT][ACGT]^
              329  CAGTG               
TthHB8I       168  TCGA                 T^CGA
              311  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AluI        BsaJI       BsiYI       BslI        BsrI        
BssKI       BstNI       DpnII       EcoRII      Fnu4HI      
HinfI       ItaI        MaeIII      MboI        MnlI        
MvaI        NdeII       NlaIV       Sau3AI      ScrFI       
SfcI        
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