(Linear) (Six-base) MAPSORT of: KIBB6788.seq  Check: 1867  from: 1  to: 462



KIBB6788.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      63     462 
   Size:         63     399
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     307     462 
   Size:        202     105     155

  Fragments arranged by size: 

                 202     155     105
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      95     462 
   Size:         95     367
 
ApoI r'AATT_y

Cuts at:      0     349     462 
   Size:        349     113
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     352     386     462 
   Size:        352      34      76

  Fragments arranged by size: 

                 352      76      34
 
BlpI GC'TnA_GC

Cuts at:      0     441     462 
   Size:        441      21
 
Bpu10I CC'TnA_GC

Cuts at:      0     431     462 
   Size:        431      31
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     462 
   Size:        462
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     133     163     218     248     440     462 
   Size:        133      30      55      30     192      22

  Fragments arranged by size: 

                 192     133      55      30      30      22
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     239     462 
   Size:        239     223
 
BseYI C'CCAG_C

Cuts at:      0     424     462 
   Size:        424      38
 
BsiEI CG_ry'CG

Cuts at:      0      18     462 
   Size:         18     444
 
BsiHKAI G_wGCw'C

Cuts at:      0     161     462 
   Size:        161     301
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     379     462 
   Size:        379      83
 
Bsp1286I G_dGCh'C

Cuts at:      0     161     462 
   Size:        161     301
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      63     462 
   Size:         63     399
 
BsrBI CCG'CTC

Cuts at:      0      41     462 
   Size:         41     421
 
BsrFI r'CCGG_y

Cuts at:      0      22     462 
   Size:         22     440
 
ClaI AT'CG_AT

Cuts at:      0     173     462 
   Size:        173     289
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     462 
   Size:         19     443
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     307     462 
   Size:        202     105     155

  Fragments arranged by size: 

                 202     155     105
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     100     462 
   Size:        100     362
 
EcoRI G'AATT_C

Cuts at:      0     349     462 
   Size:        349     113
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     440     462 
   Size:        440      22
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     201     462 
   Size:        119      82     261

  Fragments arranged by size: 

                 261     119      82
 
MspA1I CmG'CkG

Cuts at:      0      14      35     133     445     462 
   Size:         14      21      98     312      17

  Fragments arranged by size: 

                 312      98      21      17      14
 
NaeI GCC'GGC

Cuts at:      0      24     462 
   Size:         24     438
 
NgoMIV G'CCGG_C

Cuts at:      0      22     462 
   Size:         22     440
 
PfoI T'CCnGG_A

Cuts at:      0     353     462 
   Size:        353     109
 
PvuII CAG'CTG

Cuts at:      0     133     445     462 
   Size:        133     312      17

  Fragments arranged by size: 

                 312     133      17
 
StuI AGG'CCT

Cuts at:      0     116     462 
   Size:        116     346
 
StyI C'CwwG_G

Cuts at:      0     404     462 
   Size:        404      58
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     218     250     462 
   Size:        218      32     212

  Fragments arranged by size: 

                 218     212      32
 

 Enzymes that do cut:

     AarI     AcuI     AlfI     ApoI     BcgI     BlpI   Bpu10I     BsaI
    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI
    BsrBI    BsrFI     ClaI     EciI  Eco57MI    EcoNI    EcoRI    Hin4I
     MmeI   MspA1I     NaeI   NgoMIV     PfoI    PvuII     StuI     StyI
     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI     BsgI    BsiWI     BsmI    BspEI    BspHI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI  EcoICRI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     MslI     NarI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI