Restriction map for pF1KA0347
 (Linear) (Six-base) MAPSORT of: KIAA0347.seq  Check: 7436  from: 1  to: 3765



KIAA0347.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     429    1741    3765 
   Size:        429    1312    2024

  Fragments arranged by size: 

                2024    1312     429
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373     648    1828    3526    3765 
   Size:        373     275    1180    1698     239

  Fragments arranged by size: 

                1698    1180     373     275     239
 
AfeI AGC'GCT

Cuts at:      0    1844    3765 
   Size:       1844    1921
 
AflIII A'CryG_T

Cuts at:      0    1325    2504    3765 
   Size:       1325    1179    1261

  Fragments arranged by size: 

                1325    1261    1179
 
AleI CACnn'nnGTG

Cuts at:      0     494    1115    3765 
   Size:        494     621    2650

  Fragments arranged by size: 

                2650     621     494
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2681    3765 
   Size:       2681    1084
 
AlwNI CAG_nnn'CTG

Cuts at:      0     170     471    1080    2590    2635    3049    3558    3765 
   Size:        170     301     609    1510      45     414     509     207

  Fragments arranged by size: 

                1510     609     509     414     301     207     170      45
            
 
ApaI G_GGCC'C

Cuts at:      0    1312    2119    2447    3765 
   Size:       1312     807     328    1318

  Fragments arranged by size: 

                1318    1312     807     328
 
ApaLI G'TGCA_C

Cuts at:      0     949    3765 
   Size:        949    2816
 
ApoI r'AATT_y

Cuts at:      0      16     974     992    1544    3468    3765 
   Size:         16     958      18     552    1924     297

  Fragments arranged by size: 

                1924     958     552     297      18      16
 
AvaI C'yCGr_G

Cuts at:      0    1702    2411    2742    2894    3080    3204    3261    3765 
   Size:       1702     709     331     152     186     124      57     504

  Fragments arranged by size: 

                1702     709     504     331     186     152     124      57
            
 
BamHI G'GATC_C

Cuts at:      0    3399    3730    3765 
   Size:       3399     331      35

  Fragments arranged by size: 

                3399     331      35
 
BanI G'GyrC_C

Cuts at:      0     693    2607    2700    3099    3765 
   Size:        693    1914      93     399     666

  Fragments arranged by size: 

                1914     693     666     399      93
 
BanII G_rGCy'C

Cuts at:      0      53      62    1312    1385    2119    2156    2447    3211 
   Size:         53       9    1250      73     734      37     291     764

Cuts at:   3211    3235    3765 
   Size:         24     530

  Fragments arranged by size: 

                1250     764     734     530     291      73      53      37
                  24       9
 
BbeI G_GCGC'C

Cuts at:      0     697    2704    3103    3765 
   Size:        697    2007     399     662

  Fragments arranged by size: 

                2007     697     662     399
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     296    3725    3765 
   Size:        296    3429      40

  Fragments arranged by size: 

                3429     296      40
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     559     593    2007    2041    3765 
   Size:        559      34    1414      34    1724

  Fragments arranged by size: 

                1724    1414     559      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0       1    3765 
   Size:          1    3764
 
BfrBI ATG'CAT

Cuts at:      0     791    3765 
   Size:        791    2974
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2114    2698    2896    3765 
   Size:       2114     584     198     869

  Fragments arranged by size: 

                2114     869     584     198
 
BlpI GC'TnA_GC

Cuts at:      0    3198    3765 
   Size:       3198     567
 
Bme1580I G_kGCm'C

Cuts at:      0     512     953    1312    2119    2447    2534    2645    3765 
   Size:        512     441     359     807     328      87     111    1120

  Fragments arranged by size: 

                1120     807     512     441     359     328     111      87
            
 
BmgBI CAC'GTC

Cuts at:      0     507    1326    2507    3155    3612    3765 
   Size:        507     819    1181     648     457     153

  Fragments arranged by size: 

                1181     819     648     507     457     153
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      26    1087    2505    2539    2720    3253    3765 
   Size:         26    1061    1418      34     181     533     512

  Fragments arranged by size: 

                1418    1061     533     512     181      34      26
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1929    2841    3765 
   Size:       1929     912     924

  Fragments arranged by size: 

                1929     924     912
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      96    1419    1482    1619    1899    1916    2638    3007 
   Size:         96    1323      63     137     280      17     722     369

Cuts at:   3007    3032    3508    3765 
   Size:         25     476     257

  Fragments arranged by size: 

                1323     722     476     369     280     257     137      96
                  63      25      17
 
Bpu10I CC'TnA_GC

Cuts at:      0    1846    3765 
   Size:       1846    1919
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     767    3765 
   Size:        767    2998
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     118    2140    3765 
   Size:        118    2022    1625

  Fragments arranged by size: 

                2022    1625     118
 
BsaHI Gr'CG_yC

Cuts at:      0     694     874    1990    2701    3100    3143    3254    3765 
   Size:        694     180    1116     711     399      43     111     511

  Fragments arranged by size: 

                1116     711     694     511     399     180     111      43
            
 
BsaWI w'CCGG_w

Cuts at:      0     213     827    2344    2431    3765 
   Size:        213     614    1517      87    1334

  Fragments arranged by size: 

                1517    1334     614     213      87
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     491     496     521     526    1417    1447    3506    3536 
   Size:        491       5      25       5     891      30    2059      30

Cuts at:   3536    3765 
   Size:        229

  Fragments arranged by size: 

                2059     891     491     229      30      30      25       5
                   5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     541     811    1366    1485    1548    1720    2224    3312 
   Size:        541     270     555     119      63     172     504    1088

Cuts at:   3312    3765 
   Size:        453

  Fragments arranged by size: 

                1088     555     541     504     453     270     172     119
                  63
 
BseYI C'CCAG_C

Cuts at:      0      25      62      67     192    1369    1644    1827    1839 
   Size:         25      37       5     125    1177     275     183      12

Cuts at:   1839    2166    2523    2776    2848    3765 
   Size:        327     357     253      72     917

  Fragments arranged by size: 

                1177     917     357     327     275     253     183     125
                  72      37      25      12       5
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     780    1123    1329    1348    2028    2177    3765 
   Size:        780     343     206      19     680     149    1588

  Fragments arranged by size: 

                1588     780     680     343     206     149      19
 
BsiHKAI G_wGCw'C

Cuts at:      0     554     953    1100    1385    1478    2431    3765 
   Size:        554     399     147     285      93     953    1334

  Fragments arranged by size: 

                1334     953     554     399     285     147      93
 
BsmI GAATG_Cn'

Cuts at:      0     570     793    1373    2713    3765 
   Size:        570     223     580    1340    1052

  Fragments arranged by size: 

                1340    1052     580     570     223
 
Bsp1286I G_dGCh'C

Cuts at:      0      53      62     512     554     953    1100    1312    1385 
   Size:         53       9     450      42     399     147     212      73

Cuts at:   1385    1478    2119    2156    2431    2447    2534    2645    3211 
   Size:         93     641      37     275      16      87     111     566

Cuts at:   3211    3235    3765 
   Size:         24     530

  Fragments arranged by size: 

                 641     566     530     450     399     275     212     147
                 111      93      87      73      53      42      37      24
                  16       9
 
BspEI T'CCGG_A

Cuts at:      0     827    2344    3765 
   Size:        827    1517    1421

  Fragments arranged by size: 

                1517    1421     827
 
BspHI T'CATG_A

Cuts at:      0    1590    3440    3765 
   Size:       1590    1850     325

  Fragments arranged by size: 

                1850    1590     325
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     429    1741    2765    2984    3765 
   Size:        429    1312    1024     219     781

  Fragments arranged by size: 

                1312    1024     781     429     219
 
BsrBI CCG'CTC

Cuts at:      0    2937    3765 
   Size:       2937     828
 
BsrDI GCAATG_nn'

Cuts at:      0    1797    3765 
   Size:       1797    1968
 
BsrFI r'CCGG_y

Cuts at:      0    1400    3765 
   Size:       1400    2365
 
BsrGI T'GTAC_A

Cuts at:      0     614    3765 
   Size:        614    3151
 
BssSI C'ACGA_G

Cuts at:      0     838    1099    1471    3765 
   Size:        838     261     372    2294

  Fragments arranged by size: 

                2294     838     372     261
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    3558    3765 
   Size:       3558     207
 
BstBI TT'CG_AA

Cuts at:      0     307    3765 
   Size:        307    3458
 
BstEII G'GTnAC_C

Cuts at:      0     487    2492    3765 
   Size:        487    2005    1273

  Fragments arranged by size: 

                2005    1273     487
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     402     681    3612    3765 
   Size:        402     279    2931     153

  Fragments arranged by size: 

                2931     402     279     153
 
BstYI r'GATC_y

Cuts at:      0     608    1151    1394    2293    2434    3399    3730    3765 
   Size:        608     543     243     899     141     965     331      35

  Fragments arranged by size: 

                 965     899     608     543     331     243     141      35
            
 
Bsu36I CC'TnA_GG

Cuts at:      0     424    1073    2999    3765 
   Size:        424     649    1926     766

  Fragments arranged by size: 

                1926     766     649     424
 
BtgI C'CryG_G

Cuts at:      0      53     143     521    1258    1670    2645    2915    3017 
   Size:         53      90     378     737     412     975     270     102

Cuts at:   3017    3765 
   Size:        748

  Fragments arranged by size: 

                 975     748     737     412     378     270     102      90
                  53
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     683    3765 
   Size:        683    3082
 
BtsI GCAGTG_nn'

Cuts at:      0      79     111     171     768    1963    2268    2563    3765 
   Size:         79      32      60     597    1195     305     295    1202

  Fragments arranged by size: 

                1202    1195     597     305     295      79      60      32
            
 
DraIII CAC_nnn'GTG

Cuts at:      0     558     705    3765 
   Size:        558     147    3060

  Fragments arranged by size: 

                3060     558     147
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    3436    3765 
   Size:       3436     329
 
EaeI y'GGCC_r

Cuts at:      0     404     584    1136    1512    2912    3765 
   Size:        404     180     552     376    1400     853

  Fragments arranged by size: 

                1400     853     552     404     376     180
 
EarI CTCTTCn'nnn_

Cuts at:      0     449    1804    3749    3765 
   Size:        449    1355    1945      16

  Fragments arranged by size: 

                1945    1355     449      16
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      11    2442    3073    3765 
   Size:         11    2431     631     692

  Fragments arranged by size: 

                2431     692     631      11
 
EcoICRI GAG'CTC

Cuts at:      0    1383    3765 
   Size:       1383    2382
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      96     373     648    1419    1482    1619    1828    1899 
   Size:         96     277     275     771      63     137     209      71

Cuts at:   1899    1916    2638    3007    3032    3508    3526    3765 
   Size:         17     722     369      25     476      18     239

  Fragments arranged by size: 

                 771     722     476     369     277     275     239     209
                 137      96      71      63      25      18      17
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     886    1072    2176    2761    3765 
   Size:        886     186    1104     585    1004

  Fragments arranged by size: 

                1104    1004     886     585     186
 
EcoO109I rG'GnC_Cy

Cuts at:      0      75    1077    1124    1309    1359    1466    2116    2443 
   Size:         75    1002      47     185      50     107     650     327

Cuts at:   2443    2747    2924    3189    3765 
   Size:        304     177     265     576

  Fragments arranged by size: 

                1002     650     576     327     304     265     185     177
                 107      75      50      47
 
EcoRI G'AATT_C

Cuts at:      0     974    3765 
   Size:        974    2791
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     762    2172    2357    3765 
   Size:        762    1410     185    1408

  Fragments arranged by size: 

                1410    1408     762     185
 
HaeII r_GCGC'y

Cuts at:      0     697    1846    2584    2704    3103    3765 
   Size:        697    1149     738     120     399     662

  Fragments arranged by size: 

                1149     738     697     662     399     120
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     496     528     787     819    1205    1237    3106    3138 
   Size:        496      32     259      32     386      32    1869      32

Cuts at:   3138    3181    3213    3765 
   Size:         43      32     552

  Fragments arranged by size: 

                1869     552     496     386     259      43      32      32
                  32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     739    3765 
   Size:        739    3026
 
KasI G'GCGC_C

Cuts at:      0     693    2700    3099    3765 
   Size:        693    2007     399     666

  Fragments arranged by size: 

                2007     693     666     399
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1208    3686    3765 
   Size:       1208    2478      79

  Fragments arranged by size: 

                2478    1208      79
 
MscI TGG'CCA

Cuts at:      0     406    1138    3765 
   Size:        406     732    2627

  Fragments arranged by size: 

                2627     732     406
 
MslI CAynn'nnrTG

Cuts at:      0     494     842    1115    3358    3610    3765 
   Size:        494     348     273    2243     252     155

  Fragments arranged by size: 

                2243     494     348     273     252     155
 
MspA1I CmG'CkG

Cuts at:      0     468    1238    1430    1648    1754    2466    2519    2985 
   Size:        468     770     192     218     106     712      53     466

Cuts at:   2985    3049    3456    3765 
   Size:         64     407     309

  Fragments arranged by size: 

                 770     712     468     466     407     309     218     192
                 106      64      53
 
NarI GG'CG_CC

Cuts at:      0     694    2701    3100    3765 
   Size:        694    2007     399     665

  Fragments arranged by size: 

                2007     694     665     399
 
NcoI C'CATG_G

Cuts at:      0    1258    1670    2915    3017    3765 
   Size:       1258     412    1245     102     748

  Fragments arranged by size: 

                1258    1245     748     412     102
 
NdeI CA'TA_TG

Cuts at:      0    3666    3765 
   Size:       3666      99
 
NsiI A_TGCA'T

Cuts at:      0     793    3765 
   Size:        793    2972
 
NspI r_CATG'y

Cuts at:      0     759    3765 
   Size:        759    3006
 
PasI CC'CwG_GG

Cuts at:      0    1882    3765 
   Size:       1882    1883
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1035    1894    2489    3765 
   Size:       1035     859     595    1276

  Fragments arranged by size: 

                1276    1035     859     595
 
PfoI T'CCnGG_A

Cuts at:      0    1028    3765 
   Size:       1028    2737
 
PpuMI rG'GwC_Cy

Cuts at:      0      75    1077    1466    2747    3189    3765 
   Size:         75    1002     389    1281     442     576

  Fragments arranged by size: 

                1281    1002     576     442     389      75
 
PshAI GACnn'nnGTC

Cuts at:      0    3287    3765 
   Size:       3287     478
 
PspOMI G'GGCC_C

Cuts at:      0    1308    2115    2443    3765 
   Size:       1308     807     328    1322

  Fragments arranged by size: 

                1322    1308     807     328
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1201    1233    3765 
   Size:       1201      32    2532

  Fragments arranged by size: 

                2532    1201      32
 
PstI C_TGCA'G

Cuts at:      0      86     292     372    1160    1415    2228    2969    2984 
   Size:         86     206      80     788     255     813     741      15

Cuts at:   2984    3398    3765 
   Size:        414     367

  Fragments arranged by size: 

                 813     788     741     414     367     255     206      86
                  80      15
 
PvuII CAG'CTG

Cuts at:      0     468    1238    1648    1754    2519    2985    3049    3456 
   Size:        468     770     410     106     765     466      64     407

Cuts at:   3456    3765 
   Size:        309

  Fragments arranged by size: 

                 770     765     468     466     410     407     309     106
                  64
 
SacI G_AGCT'C

Cuts at:      0    1385    3765 
   Size:       1385    2380
 
SanDI GG'GwC_CC

Cuts at:      0    1466    2747    3765 
   Size:       1466    1281    1018

  Fragments arranged by size: 

                1466    1281    1018
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3749    3765 
   Size:       3749      16
 
SbfI CC_TGCA'GG

Cuts at:      0    3398    3765 
   Size:       3398     367
 
SexAI A'CCwGG_T

Cuts at:      0     626     881    3765 
   Size:        626     255    2884

  Fragments arranged by size: 

                2884     626     255
 
SfcI C'TryA_G

Cuts at:      0      82     288     368    1156    1411    2013    2224    2965 
   Size:         82     206      80     788     255     602     211     741

Cuts at:   2965    2980    3050    3394    3514    3765 
   Size:         15      70     344     120     251

  Fragments arranged by size: 

                 788     741     602     344     255     251     211     206
                 120      82      80      70      15
 
SfoI GGC'GCC

Cuts at:      0     695    2702    3101    3765 
   Size:        695    2007     399     664

  Fragments arranged by size: 

                2007     695     664     399
 
SmaI CCC'GGG

Cuts at:      0    2896    3765 
   Size:       2896     869
 
SmlI C'TyrA_G

Cuts at:      0     782    2411    3765 
   Size:        782    1629    1354

  Fragments arranged by size: 

                1629    1354     782
 
SspI AAT'ATT

Cuts at:      0    3304    3654    3765 
   Size:       3304     350     111

  Fragments arranged by size: 

                3304     350     111
 
StuI AGG'CCT

Cuts at:      0    2767    3765 
   Size:       2767     998
 
StyI C'CwwG_G

Cuts at:      0      44     383    1258    1670    2177    2297    2359    2915 
   Size:         44     339     875     412     507     120      62     556

Cuts at:   2915    3017    3765 
   Size:        102     748

  Fragments arranged by size: 

                 875     748     556     507     412     339     120     102
                  62      44
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1021    3765 
   Size:       1021    2744
 
TatI w'GTAC_w

Cuts at:      0     614    1217    3765 
   Size:        614     603    2548

  Fragments arranged by size: 

                2548     614     603
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     491     523    1417    1449    3765 
   Size:        491      32     894      32    2316

  Fragments arranged by size: 

                2316     894     491      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1765    3765 
   Size:       1765    2000
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      51    2031    3765 
   Size:         51    1980    1734

  Fragments arranged by size: 

                1980    1734      51
 
XhoI C'TCGA_G

Cuts at:      0    2411    3765 
   Size:       2411    1354
 
XmaI C'CCGG_G

Cuts at:      0    2894    3765 
   Size:       2894     871
 
XmnI GAAnn'nnTTC

Cuts at:      0     805    2340    2352    3677    3765 
   Size:        805    1535      12    1325      88

  Fragments arranged by size: 

                1535    1325     805      88      12
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AleI     AlfI    AlwNI     ApaI
    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI
     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI   DraIII
     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI    HaeII    Hin4I  HindIII     KasI     MmeI     MscI
     MslI   MspA1I     NarI     NcoI     NdeI     NsiI     NspI     PasI
    PflMI     PfoI    PpuMI    PshAI   PspOMI     PsrI     PstI    PvuII
     SacI    SanDI     SapI     SbfI    SexAI     SfcI     SfoI     SmaI
     SmlI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AloI
     AscI     AseI    AsiSI    AvrII     BaeI    BbvCI     BclI    BglII
     BmtI    BsaAI    BsaBI    BsiEI    BsiWI    BsmBI   BssHII  BstZ17I
     ClaI    CspCI     DraI     EagI    EcoRV     FseI     FspI    FspAI
   HincII     HpaI     KpnI     MfeI     MluI     NaeI   NgoMIV     NheI
     NotI     NruI     PacI     PciI     PmeI     PmlI     PpiI     PsiI
    PspXI     PvuI    RsrII    SacII     SalI     ScaI     SfiI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SwaI     XbaI     ZraI
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