Restriction map for pF1KE0010
 (Linear) (Six-base) MAPSORT of: KIEE0010.seq  Check: 6150  from: 1  to: 2310



KIEE0010.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1025    2310 
   Size:       1025    1285
 
AccI GT'mk_AC

Cuts at:      0    1086    2310 
   Size:       1086    1224
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     407    1358    2310 
   Size:        407     951     952

  Fragments arranged by size: 

                 952     951     407
 
AflII C'TTAA_G

Cuts at:      0    1704    2310 
   Size:       1704     606
 
AleI CACnn'nnGTG

Cuts at:      0    2032    2310 
   Size:       2032     278
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     374     406    2137    2169    2310 
   Size:        374      32    1731      32     141

  Fragments arranged by size: 

                1731     374     141      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      63     374     442     624    1336    2110    2265    2310 
   Size:         63     311      68     182     712     774     155      45

  Fragments arranged by size: 

                 774     712     311     182     155      68      63      45
            
 
ApoI r'AATT_y

Cuts at:      0     363    1682    2310 
   Size:        363    1319     628

  Fragments arranged by size: 

                1319     628     363
 
BamHI G'GATC_C

Cuts at:      0    1194    2310 
   Size:       1194    1116
 
BanI G'GyrC_C

Cuts at:      0     577    2310 
   Size:        577    1733
 
BanII G_rGCy'C

Cuts at:      0    1441    2310 
   Size:       1441     869
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2096    2310 
   Size:       2096     214
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1777    2297    2310 
   Size:       1777     520      13

  Fragments arranged by size: 

                1777     520      13
 
BclI T'GATC_A

Cuts at:      0      87    2310 
   Size:         87    2223
 
BfrBI ATG'CAT

Cuts at:      0      52    2310 
   Size:         52    2258
 
BglI GCCn_nnn'nGGC

Cuts at:      0     468    2310 
   Size:        468    1842
 
BglII A'GATC_T

Cuts at:      0    1030    2310 
   Size:       1030    1280
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      73    2310 
   Size:         73    2237
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     713    2310 
   Size:        713    1597
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     766    1339    1426    2023    2296    2310 
   Size:        766     573      87     597     273      14

  Fragments arranged by size: 

                 766     597     573     273      87      14
 
Bpu10I CC'TnA_GC

Cuts at:      0     422    2077    2310 
   Size:        422    1655     233

  Fragments arranged by size: 

                1655     422     233
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1242    2310 
   Size:       1242    1068
 
BsaWI w'CCGG_w

Cuts at:      0    1053    1163    2310 
   Size:       1053     110    1147

  Fragments arranged by size: 

                1147    1053     110
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     966     996    1312    1342    1613    1643    2137    2167 
   Size:        966      30     316      30     271      30     494      30

Cuts at:   2167    2310 
   Size:        143

  Fragments arranged by size: 

                 966     494     316     271     143      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     262     818    1569    2056    2310 
   Size:        262     556     751     487     254

  Fragments arranged by size: 

                 751     556     487     262     254
 
BseYI C'CCAG_C

Cuts at:      0     604     835     930    2310 
   Size:        604     231      95    1380

  Fragments arranged by size: 

                1380     604     231      95
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     140     343    2015    2310 
   Size:        140     203    1672     295

  Fragments arranged by size: 

                1672     295     203     140
 
BsiHKAI G_wGCw'C

Cuts at:      0     792     810     963    1412    2039    2310 
   Size:        792      18     153     449     627     271

  Fragments arranged by size: 

                 792     627     449     271     153      18
 
Bsp1286I G_dGCh'C

Cuts at:      0     792     810     963    1412    1441    2039    2310 
   Size:        792      18     153     449      29     598     271

  Fragments arranged by size: 

                 792     598     449     271     153      29      18
 
BspHI T'CATG_A

Cuts at:      0    1838    2310 
   Size:       1838     472
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1025    1662    2310 
   Size:       1025     637     648

  Fragments arranged by size: 

                1025     648     637
 
BsrDI GCAATG_nn'

Cuts at:      0     213    2310 
   Size:        213    2097
 
BsrFI r'CCGG_y

Cuts at:      0    2255    2310 
   Size:       2255      55
 
BsrGI T'GTAC_A

Cuts at:      0    1752    2310 
   Size:       1752     558
 
BssSI C'ACGA_G

Cuts at:      0    2165    2310 
   Size:       2165     145
 
BstEII G'GTnAC_C

Cuts at:      0     238     739    2310 
   Size:        238     501    1571

  Fragments arranged by size: 

                1571     501     238
 
BstYI r'GATC_y

Cuts at:      0     300     382    1030    1194    2310 
   Size:        300      82     648     164    1116

  Fragments arranged by size: 

                1116     648     300     164      82
 
Bsu36I CC'TnA_GG

Cuts at:      0    1391    2310 
   Size:       1391     919
 
BtgI C'CryG_G

Cuts at:      0    2082    2310 
   Size:       2082     228
 
BtsI GCAGTG_nn'

Cuts at:      0     326     412     720    2027    2059    2310 
   Size:        326      86     308    1307      32     251

  Fragments arranged by size: 

                1307     326     308     251      86      32
 
EaeI y'GGCC_r

Cuts at:      0    1397    2198    2310 
   Size:       1397     801     112

  Fragments arranged by size: 

                1397     801     112
 
EarI CTCTTCn'nnn_

Cuts at:      0      74     732    1640    1774    2310 
   Size:         74     658     908     134     536

  Fragments arranged by size: 

                 908     658     536     134      74
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     407     766    1339    1358    1426    2023    2296    2310 
   Size:        407     359     573      19      68     597     273      14

  Fragments arranged by size: 

                 597     573     407     359     273      68      19      14
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1004    2310 
   Size:       1004    1306
 
EcoO109I rG'GnC_Cy

Cuts at:      0     470     498     852    1009    1038    2310 
   Size:        470      28     354     157      29    1272

  Fragments arranged by size: 

                1272     470     354     157      29      28
 
EcoRI G'AATT_C

Cuts at:      0     363    2310 
   Size:        363    1947
 
HaeII r_GCGC'y

Cuts at:      0     156    2310 
   Size:        156    2154
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     612     644     679     711    1158    1190    1222    1254 
   Size:        612      32      35      32     447      32      32      32

Cuts at:   1254    1312    1344    1613    1645    2310 
   Size:         58      32     269      32     665

  Fragments arranged by size: 

                 665     612     447     269      58      35      32      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     530    1087    2253    2310 
   Size:        530     557    1166      57

  Fragments arranged by size: 

                1166     557     530      57
 
HindIII A'AGCT_T

Cuts at:      0    1378    1450    2310 
   Size:       1378      72     860

  Fragments arranged by size: 

                1378     860      72
 
MscI TGG'CCA

Cuts at:      0    1399    2200    2310 
   Size:       1399     801     110

  Fragments arranged by size: 

                1399     801     110
 
MslI CAynn'nnrTG

Cuts at:      0    1837    2032    2310 
   Size:       1837     195     278

  Fragments arranged by size: 

                1837     278     195
 
MspA1I CmG'CkG

Cuts at:      0    1221    1333    1828    2310 
   Size:       1221     112     495     482

  Fragments arranged by size: 

                1221     495     482     112
 
NdeI CA'TA_TG

Cuts at:      0    1200    2310 
   Size:       1200    1110
 
NsiI A_TGCA'T

Cuts at:      0      54    2310 
   Size:         54    2256
 
NspI r_CATG'y

Cuts at:      0    1695    2310 
   Size:       1695     615
 
PasI CC'CwG_GG

Cuts at:      0     477    2310 
   Size:        477    1833
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     374    2310 
   Size:        374    1936
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2137    2169    2310 
   Size:       2137      32     141

  Fragments arranged by size: 

                2137     141      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     498     852    1009    2310 
   Size:        498     354     157    1301

  Fragments arranged by size: 

                1301     498     354     157
 
PshAI GACnn'nnGTC

Cuts at:      0     828    2310 
   Size:        828    1482
 
PsiI TTA'TAA

Cuts at:      0    1573    2310 
   Size:       1573     737
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     500     532    2310 
   Size:        500      32    1778

  Fragments arranged by size: 

                1778     500      32
 
PstI C_TGCA'G

Cuts at:      0     715    2291    2310 
   Size:        715    1576      19

  Fragments arranged by size: 

                1576     715      19
 
PvuII CAG'CTG

Cuts at:      0    1333    1828    2310 
   Size:       1333     495     482

  Fragments arranged by size: 

                1333     495     482
 
SalI G'TCGA_C

Cuts at:      0    1085    2310 
   Size:       1085    1225
 
SanDI GG'GwC_CC

Cuts at:      0     852    2310 
   Size:        852    1458
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1640    2310 
   Size:       1640     670
 
SfcI C'TryA_G

Cuts at:      0     434     711     814    1587    2095    2114    2287    2310 
   Size:        434     277     103     773     508      19     173      23

  Fragments arranged by size: 

                 773     508     434     277     173     103      23      19
            
 
SmlI C'TyrA_G

Cuts at:      0    1704    2310 
   Size:       1704     606
 
StuI AGG'CCT

Cuts at:      0    1734    2310 
   Size:       1734     576
 
StyI C'CwwG_G

Cuts at:      0     520    2310 
   Size:        520    1790
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     226     628    2310 
   Size:        226     402    1682

  Fragments arranged by size: 

                1682     402     226
 
TatI w'GTAC_w

Cuts at:      0    1752    2310 
   Size:       1752     558
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     926     958    2310 
   Size:        926      32    1352

  Fragments arranged by size: 

                1352     926      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2047    2310 
   Size:       2047     263
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII     AleI     AloI    AlwNI     ApoI
    BamHI     BanI    BanII     BbsI    BciVI     BclI    BfrBI     BglI
    BglII     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI
    BsrFI    BsrGI    BssSI   BstEII    BstYI   Bsu36I     BtgI     BtsI
     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII    Hin4I
   HincII  HindIII     MscI     MslI   MspA1I     NdeI     NsiI     NspI
     PasI    PflMI     PpiI    PpuMI    PshAI     PsiI     PsrI     PstI
    PvuII     SalI    SanDI     SapI     SfcI     SmlI     StuI     StyI
    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI     AlfI
     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BbeI    BbvCI     BcgI     BlpI Bme1580I    BmgBI     BmtI    BpuEI
    BsaAI    BsaBI    BsaHI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BsrBI   BssHII   BstAPI    BstBI    BstXI  BstZ17I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoRV
     FalI     FseI     FspI    FspAI     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     PacI     PciI     PfoI     PmeI     PmlI   PspOMI    PspXI
     PvuI    RsrII     SacI    SacII     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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