Restriction map for pF1KE0371
##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AflII       
AgeI        AhdI        AocI        Aor51HI     ApoI        
AscI        AseI        AsnI        Asp700I     AspEI       
AspI        AviII       AvrII       BbrPI       BbvCI       
BciVI       BclI        BfaI        BfrI        BlnI        
BsaBI       BsaWI       BseAI       BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        Eam1105I    Eco47III    Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HindIII     HpaI        KspI        MaeI        
MaeIII      MamI        MfeI        MluI        MroI        
MseI        MunI        NaeI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        PvuII       RsrII       SacI        
SacII       SalI        SbfI        ScaI        SfiI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tru9I       Tsp45I      Tsp509I     Tth111I     Van91I      
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

Acc65I        270  GGTACC               G^GTACC
AccI         1672  GTCTAC               GT^[AC][GT]AC
Alw44I        577  GTGCAC               G^TGCAC
ApaLI         577  GTGCAC               G^TGCAC
Asp718I       270  GGTACC               G^GTACC
AspHI         577  GTGCAC               G[AT]GC[AT]^C
BamHI         227  GGATCC               G^GATCC
BbeI           42  GGCGCC               GGCGC^C
BcgI          203  CGATGATGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         701  AGATCT               A^GATCT
BlpI         1294  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1294  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1675  TACGTG               [CT]AC^GT[GA]
BsiHKAI       577  GTGCAC               G[AT]GC[AT]^C
BspHI        1226  TCATGA               T^CATGA
BspLU11I     1253  ACATGT               A^CATGT
BspMI        1190  ACCTGC               ACCTGC(4/8)
BsrDI        1508  GCAATG               GCAATG(2/0)
BsrFI         364  ACCGGC               [GA]^CCGG[CT]
CelII        1294  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        364  ACCGGC               [GA]^CCGG[CT]
DraIII       1648  CACGCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          652  GACAACCCGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII          42  GGCGCC               [GA]GCGC^[CT]
HincII       1609  GTCAAC               GT[CT]^[GA]AC
HindII       1609  GTCAAC               GT[CT]^[GA]AC
KasI           42  GGCGCC               G^GCGCC
KpnI          270  GGTACC               GGTAC^C
NarI           42  GGCGCC               GG^CGCC
NcoI         1067  CCATGG               C^CATGG
PpuMI        1187  AGGACCT              [GA]G^G[AT]CC[CT]
PstI          681  CTGCAG               CTGCA^G
RcaI         1226  TCATGA               T^CATGA
SapI          894  GCTCTTC              GCTCTTC(1/4)
SexAI         505  ACCTGGT              A^CC[AT]GGT
SfoI           42  GGCGCC               GGC^GCC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AcyI           42  GGCGCC               G[GA]^CG[CT]C
             1466  GACGCC              
AluI           11  AGCT                 AG^CT
              988  AGCT                
AlwNI         967  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1186  CAGGACCTG           
AvaI         1288  CCCGGG               C^[CT]CG[GA]G
             1390  CCCGGG              
BbsI          355  GTCTTC               GAAGAC(2/6)
              916  GTCTTC              
BglI         1028  GCCTGAGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1534  GCCTTCGGGGC         
BpmI          375  CTCCAG               CTGGAG(16/14)
             1666  CTGGAG              
BpuAI         355  GTCTTC               GAAGAC(2/6)
              916  GTCTTC              
BsaHI          42  GGCGCC               G[GA]^CG[CT]C
             1466  GACGCC              
BsoBI        1288  CCCGGG               C^[CT]CG[GA]G
             1390  CCCGGG              
BsrBI         942  GAGCGG               CCGCTC(-3/-3)
             1032  GAGCGG              
DdeI         1030  CTGAG                C^T[ACGT]AG
             1295  CTGAG               
DsaI          560  CCGTGG               C^C[GA][CT]GG
             1067  CCATGG              
EagI          557  CGGCCG               C^GGCCG
              689  CGGCCG              
EclXI         557  CGGCCG               C^GGCCG
              689  CGGCCG              
Eco52I        557  CGGCCG               C^GGCCG
              689  CGGCCG              
EcoNI         420  CCTCATCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1662  CCTGCTGGAGG         
HgaI          283  GACGC                GACGC(5/10)
             1466  GACGC               
Hin1I          42  GGCGCC               G[GA]^CG[CT]C
             1466  GACGCC              
MspA1I        141  CAGCGG               C[AC]G^C[GT]G
             1420  CAGCGG              
PleI          670  GACTC                GAGTC(4/5)
             1010  GACTC               
SmaI         1288  CCCGGG               CCC^GGG
             1390  CCCGGG              
SphI          645  GCATGC               GCATG^C
             1643  GCATGC              
TaqI          743  TCGA                 T^CGA
             1265  TCGA                
TthHB8I       743  TCGA                 T^CGA
             1265  TCGA                
XmaCI        1288  CCCGGG               C^CCGGG
             1390  CCCGGG              
XmaI         1288  CCCGGG               C^CCGGG
             1390  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AccII       AciI        AfaI        AflIII      AlwI        
ApaI        AvaII       BalI        BanI        BanII       
BbvI        BcnI        BmyI        BsaI        BsaJI       
BseRI       BsgI        BsiEI       BsiYI       BslI        
BsmAI       BsmBI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DpnII       
DraII       EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TfiI        TseI        TspRI       
XcmI        XhoII       
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