Restriction map for pF1KE0371
 (Linear) (Six-base) MAPSORT of: KIEE0371.seq  Check: 9245  from: 1  to: 1686



KIEE0371.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AccI GT'mk_AC

Cuts at:      0    1673    1686 
   Size:       1673      13
 
Acc65I G'GTAC_C

Cuts at:      0     270    1686 
   Size:        270    1416
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1397    1686 
   Size:       1397     289
 
AflIII A'CryG_T

Cuts at:      0     182    1253    1364    1686 
   Size:        182    1071     111     322

  Fragments arranged by size: 

                1071     322     182     111
 
AleI CACnn'nnGTG

Cuts at:      0     181    1686 
   Size:        181    1505
 
AlwNI CAG_nnn'CTG

Cuts at:      0     972    1191    1686 
   Size:        972     219     495

  Fragments arranged by size: 

                 972     495     219
 
ApaI G_GGCC'C

Cuts at:      0      25      58    1545    1686 
   Size:         25      33    1487     141

  Fragments arranged by size: 

                1487     141      33      25
 
ApaLI G'TGCA_C

Cuts at:      0     577    1686 
   Size:        577    1109
 
AvaI C'yCGr_G

Cuts at:      0    1288    1390    1686 
   Size:       1288     102     296

  Fragments arranged by size: 

                1288     296     102
 
BamHI G'GATC_C

Cuts at:      0     227    1686 
   Size:        227    1459
 
BanI G'GyrC_C

Cuts at:      0      42     270    1120    1686 
   Size:         42     228     850     566

  Fragments arranged by size: 

                 850     566     228      42
 
BanII G_rGCy'C

Cuts at:      0      25      37      58    1205    1325    1545    1686 
   Size:         25      12      21    1147     120     220     141

  Fragments arranged by size: 

                1147     220     141     120      25      21      12
 
BbeI G_GCGC'C

Cuts at:      0      46    1686 
   Size:         46    1640
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     348     909    1686 
   Size:        348     561     777

  Fragments arranged by size: 

                 777     561     348
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     192     226    1686 
   Size:        192      34    1460

  Fragments arranged by size: 

                1460     192      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1034    1540    1686 
   Size:       1034     506     146

  Fragments arranged by size: 

                1034     506     146
 
BglII A'GATC_T

Cuts at:      0     701    1686 
   Size:        701     985
 
BlpI GC'TnA_GC

Cuts at:      0    1295    1686 
   Size:       1295     391
 
Bme1580I G_kGCm'C

Cuts at:      0      25      58     581    1180    1545    1686 
   Size:         25      33     523     599     365     141

  Fragments arranged by size: 

                 599     523     365     141      33      25
 
BmgBI CAC'GTC

Cuts at:      0     183     489    1104    1686 
   Size:        183     306     615     582

  Fragments arranged by size: 

                 615     582     306     183
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     360    1686 
   Size:        360    1326
 
Bpu10I CC'TnA_GC

Cuts at:      0    1030    1686 
   Size:       1030     656
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     471     520     853    1686 
   Size:        471      49     333     833

  Fragments arranged by size: 

                 833     471     333      49
 
BsaAI yAC'GTr

Cuts at:      0    1677    1686 
   Size:       1677       9
 
BsaHI Gr'CG_yC

Cuts at:      0      43    1467    1686 
   Size:         43    1424     219

  Fragments arranged by size: 

                1424     219      43
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1573    1603    1686 
   Size:       1573      30      83

  Fragments arranged by size: 

                1573      83      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      22     112     232     680    1686 
   Size:         22      90     120     448    1006

  Fragments arranged by size: 

                1006     448     120      90      22
 
BseYI C'CCAG_C

Cuts at:      0    1235    1405    1686 
   Size:       1235     170     281

  Fragments arranged by size: 

                1235     281     170
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     114     321     397    1686 
   Size:        114     207      76    1289

  Fragments arranged by size: 

                1289     207     114      76
 
BsiEI CG_ry'CG

Cuts at:      0     560     662     692    1686 
   Size:        560     102      30     994

  Fragments arranged by size: 

                 994     560     102      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     581    1686 
   Size:        581    1105
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     634    1446    1458    1686 
   Size:        634     812      12     228

  Fragments arranged by size: 

                 812     634     228      12
 
Bsp1286I G_dGCh'C

Cuts at:      0      25      37      58     581    1180    1205    1325    1545 
   Size:         25      12      21     523     599      25     120     220

Cuts at:   1545    1686 
   Size:        141

  Fragments arranged by size: 

                 599     523     220     141     120      25      25      21
                  12
 
BspHI T'CATG_A

Cuts at:      0    1226    1686 
   Size:       1226     460
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1199    1686 
   Size:       1199     487
 
BsrBI CCG'CTC

Cuts at:      0     944    1034    1686 
   Size:        944      90     652

  Fragments arranged by size: 

                 944     652      90
 
BsrDI GCAATG_nn'

Cuts at:      0    1515    1686 
   Size:       1515     171
 
BsrFI r'CCGG_y

Cuts at:      0     364    1686 
   Size:        364    1322
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     540     552    1665    1686 
   Size:        540      12    1113      21

  Fragments arranged by size: 

                1113     540      21      12
 
BstYI r'GATC_y

Cuts at:      0     227     407     498     701    1686 
   Size:        227     180      91     203     985

  Fragments arranged by size: 

                 985     227     203     180      91
 
BtgI C'CryG_G

Cuts at:      0     560    1067    1686 
   Size:        560     507     619

  Fragments arranged by size: 

                 619     560     507
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     217    1686 
   Size:        217    1469
 
BtsI GCAGTG_nn'

Cuts at:      0     197    1219    1436    1686 
   Size:        197    1022     217     250

  Fragments arranged by size: 

                1022     250     217     197
 
DraIII CAC_nnn'GTG

Cuts at:      0    1653    1686 
   Size:       1653      33
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     658    1686 
   Size:        658    1028
 
EaeI y'GGCC_r

Cuts at:      0      81     164     542     557     689     819    1433    1686 
   Size:         81      83     378      15     132     130     614     253

  Fragments arranged by size: 

                 614     378     253     132     130      83      81      15
            
 
EagI C'GGCC_G

Cuts at:      0     557     689    1686 
   Size:        557     132     997

  Fragments arranged by size: 

                 997     557     132
 
EarI CTCTTCn'nnn_

Cuts at:      0     238     760     856     901    1686 
   Size:        238     522      96      45     785

  Fragments arranged by size: 

                 785     522     238      96      45
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     161    1362    1686 
   Size:        161    1201     324

  Fragments arranged by size: 

                1201     324     161
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     360    1397    1686 
   Size:        360    1037     289

  Fragments arranged by size: 

                1037     360     289
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     424    1666    1686 
   Size:        424    1242      20

  Fragments arranged by size: 

                1242     424      20
 
EcoO109I rG'GnC_Cy

Cuts at:      0      21      22      55     969    1188    1541    1686 
   Size:         21       1      33     914     219     353     145

  Fragments arranged by size: 

                 914     353     219     145      33      21       1
 
HaeII r_GCGC'y

Cuts at:      0      46    1686 
   Size:         46    1640
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     379     411    1686 
   Size:        379      32    1275

  Fragments arranged by size: 

                1275     379      32
 
HincII GTy'rAC

Cuts at:      0    1611    1686 
   Size:       1611      75
 
KasI G'GCGC_C

Cuts at:      0      42    1686 
   Size:         42    1644
 
KpnI G_GTAC'C

Cuts at:      0     274    1686 
   Size:        274    1412
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     642    1686 
   Size:        642    1044
 
MscI TGG'CCA

Cuts at:      0     544     821    1435    1686 
   Size:        544     277     614     251

  Fragments arranged by size: 

                 614     544     277     251
 
MslI CAynn'nnrTG

Cuts at:      0     181     202     550    1686 
   Size:        181      21     348    1136

  Fragments arranged by size: 

                1136     348     181      21
 
MspA1I CmG'CkG

Cuts at:      0     143    1422    1686 
   Size:        143    1279     264

  Fragments arranged by size: 

                1279     264     143
 
NarI GG'CG_CC

Cuts at:      0      43    1686 
   Size:         43    1643
 
NcoI C'CATG_G

Cuts at:      0    1067    1686 
   Size:       1067     619
 
NspI r_CATG'y

Cuts at:      0     649    1257    1647    1686 
   Size:        649     608     390      39

  Fragments arranged by size: 

                 649     608     390      39
 
PasI CC'CwG_GG

Cuts at:      0      18      59    1128    1686 
   Size:         18      41    1069     558

  Fragments arranged by size: 

                1069     558      41      18
 
PciI A'CATG_T

Cuts at:      0    1253    1686 
   Size:       1253     433
 
PfoI T'CCnGG_A

Cuts at:      0     854    1686 
   Size:        854     832
 
PpuMI rG'GwC_Cy

Cuts at:      0    1188    1686 
   Size:       1188     498
 
PspOMI G'GGCC_C

Cuts at:      0      21      54    1541    1686 
   Size:         21      33    1487     145

  Fragments arranged by size: 

                1487     145      33      21
 
PstI C_TGCA'G

Cuts at:      0     685    1686 
   Size:        685    1001
 
SapI GCTCTTCn'nnn_

Cuts at:      0     901    1686 
   Size:        901     785
 
SexAI A'CCwGG_T

Cuts at:      0     505    1686 
   Size:        505    1181
 
SfcI C'TryA_G

Cuts at:      0     594     681     984    1686 
   Size:        594      87     303     702

  Fragments arranged by size: 

                 702     594     303      87
 
SfoI GGC'GCC

Cuts at:      0      44    1686 
   Size:         44    1642
 
SmaI CCC'GGG

Cuts at:      0    1290    1392    1686 
   Size:       1290     102     294

  Fragments arranged by size: 

                1290     294     102
 
SphI G_CATG'C

Cuts at:      0     649    1647    1686 
   Size:        649     998      39

  Fragments arranged by size: 

                 998     649      39
 
StyI C'CwwG_G

Cuts at:      0      26    1019    1067    1316    1686 
   Size:         26     993      48     249     370

  Fragments arranged by size: 

                 993     370     249      48      26
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1108    1228    1398    1686 
   Size:       1108     120     170     288

  Fragments arranged by size: 

                1108     288     170     120
 
TatI w'GTAC_w

Cuts at:      0     746    1686 
   Size:        746     940
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      63      95    1354    1386    1639    1671    1686 
   Size:         63      32    1259      32     253      32      15

  Fragments arranged by size: 

                1259     253      63      32      32      32      15
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     969    1095    1164    1392    1686 
   Size:        969     126      69     228     294

  Fragments arranged by size: 

                 969     294     228     126      69
 
XmaI C'CCGG_G

Cuts at:      0    1288    1390    1686 
   Size:       1288     102     296

  Fragments arranged by size: 

                1288     296     102
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI   AflIII     AleI    AlwNI     ApaI    ApaLI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI     BglI
    BglII     BlpI Bme1580I    BmgBI     BpmI   Bpu10I     BsaI    BsaAI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BstXI    BstYI
     BtgI    BtgZI     BtsI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I   HincII     KasI
     KpnI     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NspI
     PasI     PciI     PfoI    PpuMI   PspOMI     PstI     SapI    SexAI
     SfcI     SfoI     SmaI     SphI     StyI    TaqII     TatI     TstI
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII     AgeI     AhdI     AlfI
     AloI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BbvCI
    BciVI     BclI    BfrBI     BmrI     BmtI     BplI    BpuEI    BsaBI
    BsaWI    BsiWI     BsmI    BspEI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI  EcoICRI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI
     MfeI     MluI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XhoI
     XmnI     ZraI
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