Restriction map for pF1KE0142
##################################
# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvrII       BalI        BamHI       BanII       BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaI        BsaWI       
BseAI       BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
ClaI        DdeI        DpnII       DraI        DraII       
DrdI        DsaI        Eam1105I    EarI        Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FbaI        FseI        HincII      
HindII      HindIII     HinfI       HpaI        HphI        
KpnI        Ksp632I     KspI        MaeII       MaeIII      
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MunI        NcoI        
NdeI        NdeII       NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        SacI        
SacII       SalI        SapI        Sau3AI      SbfI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        TfiI        Tsp45I      Tsp509I     Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   34 enzymes) #
###################################

AfaI          300  GTAC                 GT^AC
AlwNI         518  CAGTGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       402  AGCGCT               AGC^GCT
BfaI          289  CTAG                 C^TAG
BmyI          192  GGGCAC               G[AGT]GC[ACT]^C
BsaBI         171  GATGCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI          418  GTGCAG               GTGCAG(16/14)
BsiEI         498  CGGCCG               CG[GA][CT]^CG
BsmFI         368  GGGAC                GGGAC(10/14)
Bsp1286I      192  GGGCAC               G[AGT]GC[ACT]^C
BssHII        477  GCGCGC               G^CGCGC
BstF5I         45  CATCC                GGATG(2/0)
CpoI          502  CGGTCCG              CG^G[AT]CCG
EaeI          498  CGGCCG               [CT]^GGCC[GA]
EagI          498  CGGCCG               C^GGCCG
EclXI         498  CGGCCG               C^GGCCG
Eco47III      402  AGCGCT               AGC^GCT
Eco52I        498  CGGCCG               C^GGCCG
FokI           45  CATCC                GGATG(9/13)
HgaI          526  GCGTC                GACGC(5/10)
MaeI          289  CTAG                 C^TAG
MamI          171  GATGCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI           80  TTAA                 T^TAA
MspA1I        208  CCGCTG               C[AC]G^C[GT]G
NaeI          132  GCCGGC               GCC^GGC
NgoMIV        132  GCCGGC               G^CCGGC
NlaIII        180  CATG                 CATG^
PstI          406  CTGCAG               CTGCA^G
RsaI          300  GTAC                 GT^AC
RsrII         502  CGGTCCG              CG^G[AT]CCG
ScaI          299  AGTACT               AGT^ACT
SfcI          406  CTGCAG               C^T[GA][CT]AG
Tru9I          80  TTAA                 T^TAA
XcmI          263  CCATCTTCATCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AvaII         164  GGACC                G^G[AT]CC
              503  GGTCC               
AviII         173  TGCGCA               TGC^GCA
              455  TGCGCA              
BcnI          189  CCGGG                CC^[GC]GG
              496  CCCGG               
BpmI          295  CTGGAG               CTGGAG(16/14)
              445  CTGGAG              
BsaJI         189  CCGGGG               C^C[ACGT][ACGT]GG
              443  CCCTGG              
BspMI          51  GCAGGT               ACCTGC(4/8)
              414  GCAGGT              
BsrFI         132  GCCGGC               [GA]^CCGG[CT]
              500  GCCGGT              
BsrI          149  ACTGG                ACTGG(1/-1)
              302  ACTGG               
BstAPI        468  GCAAGAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              517  GCAGTGCCTGC         
Cfr10I        132  GCCGGC               [GA]^CCGG[CT]
              500  GCCGGT              
DraIII        148  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
              514  CACGCAGTG           
FspI          173  TGCGCA               TGC^GCA
              455  TGCGCA              
MboII         107  TCTTC                GAAGA(8/7)
              266  TCTTC               
NciI          189  CCGGG                CC^[GC]GG
              496  CCCGG               
TspRI         148  CACTG                CA[GC]TG[ACGT][ACGT]^
              518  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        BanI        
BbeI        BbvI        BsaHI       BseRI       BsiYI       
BslI        BssKI       BstNI       BstUI       Cac8I       
CfoI        Cfr13I      EcoRII      FauI        Fnu4HI      
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HpaII       ItaI        KasI        MnlI        
MspI        MvaI        MvnI        MwoI        NarI        
NlaIV       Sau96I      ScrFI       SfaNI       SfoI        
TseI        
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