Restriction map for pF1KE0104
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AviII       AvrII       BamHI       BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BlnI        BsaAI       
BsaI        BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CfoI        Cfr10I      ClaI        
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HhaI        HinP1I      HindIII     
KasI        KpnI        KspI        MaeII       MluI        
MroI        MvnI        NaeI        NarI        NciI        
NdeI        NgoMIV      NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PstI        PvuI        RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        TfiI        
Tth111I     XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI         1215  GTCTAC               GT^[AC][GT]AC
AcsI          715  GAATTC               [GA]^AATT[CT]
AcyI          934  GGCGTC               G[GA]^CG[CT]C
ApoI          715  GAATTC               [GA]^AATT[CT]
AseI         1350  ATTAAT               AT^TAAT
AsnI         1350  ATTAAT               AT^TAAT
AvaI          648  CCCGAG               C^[CT]CG[GA]G
BanI          170  GGCACC               G^G[CT][GA]CC
BglI         1010  GCCTTTTAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         814  AGATCT               A^GATCT
BlpI         1199  GCTTAGC              GC^T[ACGT]AGC
BmyI          169  GGGCAC               G[AGT]GC[ACT]^C
BpmI         1076  CTGGAG               CTGGAG(16/14)
Bpu1102I     1199  GCTTAGC              GC^T[ACGT]AGC
BsaBI         463  GATTTAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         934  GGCGTC               G[GA]^CG[CT]C
BsaWI         380  ACCGGA               [AT]^CCGG[AT]
BseRI         655  GAGGAG               GAGGAG(10/8)
BsgI          242  CTGCAC               GTGCAG(16/14)
BsmI         1312  GCATTC               GAATGC(1/-1)
BsoBI         648  CCCGAG               C^[CT]CG[GA]G
Bsp1286I      169  GGGCAC               G[AGT]GC[ACT]^C
CelII        1199  GCTTAGC              GC^T[ACGT]AGC
DraII        1277  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI         1165  CCATGG               C^C[GA][CT]GG
EcoO109I     1277  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         715  GAATTC               G^AATTC
FauI         1256  CCCGC                CCCGC(4/6)
HapII         381  CCGG                 C^CGG
HgaI          935  GCGTC                GACGC(5/10)
Hin1I         934  GGCGTC               G[GA]^CG[CT]C
HinfI        1127  GAGTC                G^A[ACGT]TC
HpaI           81  GTTAAC               GTT^AAC
HpaII         381  CCGG                 C^CGG
MamI          463  GATTTAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI          991  CATCCAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          381  CCGG                 C^CGG
NcoI         1165  CCATGG               C^CATGG
NheI          417  GCTAGC               G^CTAGC
NspI         1043  GCATGT               [GA]CATG^[CT]
PflMI         853  CCAACCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         1127  GAGTC                GAGTC(4/5)
PpuMI        1277  AGGTCCT              [GA]G^G[AT]CC[CT]
PshBI        1350  ATTAAT               AT^TAAT
SfcI         1217  CTACAG               C^T[GA][CT]AG
SwaI          464  ATTTAAAT             ATTT^AAAT
TaqI          837  TCGA                 T^CGA
Tsp45I        209  GTGAC                ^GT[GC]AC
TthHB8I       837  TCGA                 T^CGA
Van91I        853  CCAACCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AciI         1257  CCGC                 CCGC(-3/-1)
              157  GCGG                
AlwI          697  GATCC                GGATC(4/5)
              964  GATCC               
AlwNI          56  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1276  CAGGTCCTG           
BalI         1137  TGGCCA               TGG^CCA
             1168  TGGCCA              
BbsI          913  GAAGAC               GAAGAC(2/6)
                3  GTCTTC              
BpuAI         913  GAAGAC               GAAGAC(2/6)
                3  GTCTTC              
BsiYI         648  CCCGAGAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              853  CCAACCTGTGG         
BslI          648  CCCGAGAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              853  CCAACCTGTGG         
BsmFI        1000  GGGAC                GGGAC(10/14)
              646  GTCCC               
BstYI         696  AGATCC               [GA]^GATC[CT]
              814  AGATCT              
DraI          465  TTTAAA               TTT^AAA
              906  TTTAAA              
EaeI         1137  TGGCCA               [CT]^GGCC[GA]
             1168  TGGCCA              
EarI          110  CTCTTC               CTCTTC(1/4)
             1114  GAAGAG              
HphI          640  GGTGA                GGTGA(8/7)
              581  TCACC               
Ksp632I       110  CTCTTC               CTCTTC(1/4)
             1114  GAAGAG              
MfeI          218  CAATTG               C^AATTG
              775  CAATTG              
MflI          696  AGATCC               [GA]^GATC[CT]
              814  AGATCT              
MluNI        1137  TGGCCA               TGG^CCA
             1168  TGGCCA              
MscI         1137  TGGCCA               TGG^CCA
             1168  TGGCCA              
MunI          218  CAATTG               C^AATTG
              775  CAATTG              
PvuII          56  CAGCTG               CAG^CTG
              532  CAGCTG              
SfaNI        1093  GATGC                GCATC(5/9)
             1252  GATGC               
SmlI          196  CTTGAG               C^T[CT][GA]AG
              806  CTCAAG              
XhoII         696  AGATCC               [GA]^GATC[CT]
              814  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AluI        AvaII       BbvI        BfaI        BsaJI       
BsmAI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HincII      
HindII      ItaI        MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MspA1I      MvaI        
MwoI        NdeII       NlaIII      NlaIV       Sau3AI      
Sau96I      ScrFI       StyI        Tru9I       TseI        
Tsp509I     TspRI       
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