Restriction map for pF1KE0147
 (Linear) (Six-base) MAPSORT of: KIEE0147.seq  Check: 382  from: 1  to: 612



KIEE0147.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AfeI AGC'GCT

Cuts at:      0     362     612 
   Size:        362     250
 
AhdI GACnn_n'nnGTC

Cuts at:      0     145     612 
   Size:        145     467
 
AlwNI CAG_nnn'CTG

Cuts at:      0     115     333     612 
   Size:        115     218     279

  Fragments arranged by size: 

                 279     218     115
 
AvaI C'yCGr_G

Cuts at:      0     201     612 
   Size:        201     411
 
BanI G'GyrC_C

Cuts at:      0     337     612 
   Size:        337     275
 
BanII G_rGCy'C

Cuts at:      0     449     612 
   Size:        449     163
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     474     612 
   Size:        474     138
 
BglI GCCn_nnn'nGGC

Cuts at:      0     353     612 
   Size:        353     259
 
BlpI GC'TnA_GC

Cuts at:      0     424     612 
   Size:        424     188
 
Bme1580I G_kGCm'C

Cuts at:      0     157     260     340     612 
   Size:        157     103      80     272

  Fragments arranged by size: 

                 272     157     103      80
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     403     612 
   Size:        403     209
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289     398     612 
   Size:        289     109     214

  Fragments arranged by size: 

                 289     214     109
 
BsaAI yAC'GTr

Cuts at:      0     474     612 
   Size:        474     138
 
BsaWI w'CCGG_w

Cuts at:      0     144     612 
   Size:        144     468
 
BseYI C'CCAG_C

Cuts at:      0      43     222     612 
   Size:         43     179     390

  Fragments arranged by size: 

                 390     179      43
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     128     604     612 
   Size:         70      58     476       8

  Fragments arranged by size: 

                 476      70      58       8
 
Bsp1286I G_dGCh'C

Cuts at:      0     157     260     340     449     612 
   Size:        157     103      80     109     163

  Fragments arranged by size: 

                 163     157     109     103      80
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      92     612 
   Size:         92     520
 
BsrBI CCG'CTC

Cuts at:      0      77     612 
   Size:         77     535
 
BsrDI GCAATG_nn'

Cuts at:      0     531     612 
   Size:        531      81
 
BsrFI r'CCGG_y

Cuts at:      0     161     612 
   Size:        161     451
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     115     612 
   Size:        115     497
 
Bsu36I CC'TnA_GG

Cuts at:      0     134     612 
   Size:        134     478
 
BtgI C'CryG_G

Cuts at:      0      36     612 
   Size:         36     576
 
BtsI GCAGTG_nn'

Cuts at:      0     113     260     612 
   Size:        113     147     352

  Fragments arranged by size: 

                 352     147     113
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     513     548     612 
   Size:        513      35      64

  Fragments arranged by size: 

                 513      64      35
 
EaeI y'GGCC_r

Cuts at:      0     534     612 
   Size:        534      78
 
EarI CTCTTCn'nnn_

Cuts at:      0      97     406     612 
   Size:         97     309     206

  Fragments arranged by size: 

                 309     206      97
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289     398     612 
   Size:        289     109     214

  Fragments arranged by size: 

                 289     214     109
 
EcoO109I rG'GnC_Cy

Cuts at:      0     139     206     330     612 
   Size:        139      67     124     282

  Fragments arranged by size: 

                 282     139     124      67
 
HaeII r_GCGC'y

Cuts at:      0     364     612 
   Size:        364     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      32     131     163     612 
   Size:         32      99      32     449

  Fragments arranged by size: 

                 449      99      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     281     612 
   Size:        163     118     331

  Fragments arranged by size: 

                 331     163     118
 
MscI TGG'CCA

Cuts at:      0     536     612 
   Size:        536      76
 
MspA1I CmG'CkG

Cuts at:      0      29      38     309     376     612 
   Size:         29       9     271      67     236

  Fragments arranged by size: 

                 271     236      67      29       9
 
NaeI GCC'GGC

Cuts at:      0     163     612 
   Size:        163     449
 
NgoMIV G'CCGG_C

Cuts at:      0     161     612 
   Size:        161     451
 
PasI CC'CwG_GG

Cuts at:      0     333     612 
   Size:        333     279
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     333     612 
   Size:        333     279
 
PmlI CAC'GTG

Cuts at:      0     474     612 
   Size:        474     138
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     274     306     612 
   Size:        274      32     306

  Fragments arranged by size: 

                 306     274      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     139     206     612 
   Size:        139      67     406

  Fragments arranged by size: 

                 406     139      67
 
PstI C_TGCA'G

Cuts at:      0      28      34     612 
   Size:         28       6     578

  Fragments arranged by size: 

                 578      28       6
 
PvuII CAG'CTG

Cuts at:      0      29     376     612 
   Size:         29     347     236

  Fragments arranged by size: 

                 347     236      29
 
SacII CC_GC'GG

Cuts at:      0      39     612 
   Size:         39     573
 
SanDI GG'GwC_CC

Cuts at:      0     206     612 
   Size:        206     406
 
SfcI C'TryA_G

Cuts at:      0      24      30     612 
   Size:         24       6     582

  Fragments arranged by size: 

                 582      24       6
 
StyI C'CwwG_G

Cuts at:      0     437     449     612 
   Size:        437      12     163

  Fragments arranged by size: 

                 437     163      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     214     528     612 
   Size:        214     314      84

  Fragments arranged by size: 

                 314     214      84
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      46      78     503     535     612 
   Size:         46      32     425      32      77

  Fragments arranged by size: 

                 425      77      46      32      32
 

 Enzymes that do cut:

     AfeI     AhdI    AlwNI     AvaI     BanI    BanII     BbsI     BglI
     BlpI Bme1580I     BplI     BpmI    BsaAI    BsaWI    BseYI     BsgI
 Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI   BstAPI   Bsu36I     BtgI
     BtsI    CspCI     EaeI     EarI  Eco57MI EcoO109I    HaeII    Hin4I
     MmeI     MscI   MspA1I     NaeI   NgoMIV     PasI    PflMI     PmlI
     PpiI    PpuMI     PstI    PvuII    SacII    SanDI     SfcI     StyI
    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII    BmgBI     BmrI     BmtI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaHI    BsaXI    BseRI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI    BstYI  BstZ17I    BtgZI     ClaI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MslI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI     PfoI     PmeI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI
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