Restriction map for pF1KB7803
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# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflII       AgeI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaHI       BsaI        BseRI       
BsiEI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      Bst1107I    BstBI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoT22I     FbaI        
FseI        FspI        Hin1I       HindIII     KasI        
KpnI        KspI        MaeI        MfeI        MluI        
MslI        MunI        MvnI        NaeI        NarI        
NciI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tth111I     XbaI        
XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   41 enzymes) #
###################################

AcsI          575  GAATTC               [GA]^AATT[CT]
AflIII        325  ACACGT               A^C[GA][CT]GT
ApoI          575  GAATTC               [GA]^AATT[CT]
AspHI        1319  GAGCAC               G[AT]GC[AT]^C
AvaI          142  CCCGAG               C^[CT]CG[GA]G
BalI         1424  TGGCCA               TGG^CCA
BbvCI        1255  GCTGAGG              CCTCAGC(-5/-2)
BmyI         1319  GAGCAC               G[AGT]GC[ACT]^C
BsaBI        1101  GATGACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI      1319  GAGCAC               G[AT]GC[AT]^C
BsoBI         142  CCCGAG               C^[CT]CG[GA]G
Bsp1286I     1319  GAGCAC               G[AGT]GC[ACT]^C
BssSI         371  CTCGTG               CACGAG(-5/-1)
BstAPI        102  GCATAGTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I       1107  CATCC                GGATG(2/0)
BstYI        1358  AGATCC               [GA]^GATC[CT]
DraI          430  TTTAAA               TTT^AAA
DraII         222  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DraIII         10  CACGGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1456  GACAGCGCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I      222  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         575  GAATTC               G^AATTC
FauI          822  GCGGG                CCCGC(4/6)
FokI         1107  CATCC                GGATG(9/13)
HaeII        1212  GGCGCT               [GA]GCGC^[CT]
HgaI         1116  GACGC                GACGC(5/10)
HincII        541  GTTAAC               GT[CT]^[GA]AC
HindII        541  GTTAAC               GT[CT]^[GA]AC
HpaI          541  GTTAAC               GTT^AAC
MamI         1101  GATGACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         1358  AGATCC               [GA]^GATC[CT]
MluNI        1424  TGGCCA               TGG^CCA
MscI         1424  TGGCCA               TGG^CCA
NdeI           31  CATATG               CA^TATG
PpuMI         222  AGGTCCT              [GA]G^G[AT]CC[CT]
PshAI         912  GACTGTAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          218  CTGCAG               CTGCA^G
SapI          748  GAAGAGC              GCTCTTC(1/4)
ScaI          641  AGTACT               AGT^ACT
SmlI          637  CTCAAG               C^T[CT][GA]AG
XhoII        1358  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccIII        308  TCCGGA               T^CCGGA
              728  TCCGGA              
AhdI          496  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1280  GACAGATCGTC         
AlwI          486  GATCC                GGATC(4/5)
             1359  GATCC               
AspEI         496  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1280  GACAGATCGTC         
BpmI          499  CTGGAG               CTGGAG(16/14)
             1328  CTGGAG              
BsaWI         308  TCCGGA               [AT]^CCGG[AT]
              728  TCCGGA              
BseAI         308  TCCGGA               T^CCGGA
              728  TCCGGA              
BsmAI         583  GTCTC                GTCTC(1/5)
              949  GTCTC               
BsmFI         378  GGGAC                GGGAC(10/14)
             1278  GGGAC               
BspEI         308  TCCGGA               T^CCGGA
              728  TCCGGA              
BstEII       1020  GGTCACC              G^GT[ACGT]ACC
             1386  GGTGACC             
BstPI        1020  GGTCACC              G^GT[ACGT]ACC
             1386  GGTGACC             
Eam1105I      496  GACCTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1280  GACAGATCGTC         
EarI          312  GAAGAG               CTCTTC(1/4)
              748  GAAGAG              
EcoNI        1251  CCTGGCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1395  CCTCGCCCAGG         
EcoO65I      1020  GGTCACC              G^GT[ACGT]ACC
             1386  GGTGACC             
EcoRV         709  GATATC               GAT^ATC
             1449  GATATC              
EcoT14I      1427  CCATGG               C^C[AT][AT]GG
             1469  CCATGG              
HapII         309  CCGG                 C^CGG
              729  CCGG                
HpaII         309  CCGG                 C^CGG
              729  CCGG                
Ksp632I       312  GAAGAG               CTCTTC(1/4)
              748  GAAGAG              
MroI          308  TCCGGA               T^CCGGA
              728  TCCGGA              
MspI          309  CCGG                 C^CGG
              729  CCGG                
NcoI         1427  CCATGG               C^CATGG
             1469  CCATGG              
NlaIV         773  GGACCC               GG[ACGT]^[ACGT]CC
             1431  GGCCCC              
NspI           51  ACATGC               [GA]CATG^[CT]
             1370  GCATGT              
PvuII         188  CAGCTG               CAG^CTG
              215  CAGCTG              
StyI         1427  CCATGG               C^C[AT][AT]GG
             1469  CCATGG              
TaqI          345  TCGA                 T^CGA
              569  TCGA                
TthHB8I       345  TCGA                 T^CGA
              569  TCGA                
XcmI         1133  CCACCCAGGCGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1335  CCAAGATGCTAATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AvaII       
BbsI        BbvI        BglI        BpuAI       BsaJI       
BsgI        BsiYI       BslI        BspMI       BsrI        
BssKI       BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DsaI        EaeI        EcoRII      
Fnu4HI      HaeIII      HhaI        HinP1I      HinfI       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MspA1I      MvaI        
MwoI        NdeII       NlaIII      PflMI       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Van91I      
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