Restriction map for pF1KB7803
 (Linear) (Six-base) MAPSORT of: KIBB7803.seq  Check: 935  from: 1  to: 1509



KIBB7803.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1410    1509 
   Size:       1410      99
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     725     986    1509 
   Size:        725     261     523

  Fragments arranged by size: 

                 725     523     261
 
AflIII A'CryG_T

Cuts at:      0     325    1509 
   Size:        325    1184
 
AhdI GACnn_n'nnGTC

Cuts at:      0     501    1285    1509 
   Size:        501     784     224

  Fragments arranged by size: 

                 784     501     224
 
AlwNI CAG_nnn'CTG

Cuts at:      0     190     226     586    1509 
   Size:        190      36     360     923

  Fragments arranged by size: 

                 923     360     190      36
 
ApoI r'AATT_y

Cuts at:      0     575    1509 
   Size:        575     934
 
AvaI C'yCGr_G

Cuts at:      0     142    1509 
   Size:        142    1367
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     131     500     833    1509 
   Size:        131     369     333     676

  Fragments arranged by size: 

                 676     369     333     131
 
BbvCI CC'TCA_GC

Cuts at:      0    1256    1509 
   Size:       1256     253
 
BglI GCCn_nnn'nGGC

Cuts at:      0     991    1138    1168    1252    1509 
   Size:        991     147      30      84     257

  Fragments arranged by size: 

                 991     257     147      84      30
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     168     561    1509 
   Size:        168     393     948

  Fragments arranged by size: 

                 948     393     168
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520    1349    1509 
   Size:        520     829     160

  Fragments arranged by size: 

                 829     520     160
 
Bpu10I CC'TnA_GC

Cuts at:      0    1256    1509 
   Size:       1256     253
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     622    1509 
   Size:        622     887
 
BsaBI GATnn'nnATC

Cuts at:      0    1105    1509 
   Size:       1105     404
 
BsaWI w'CCGG_w

Cuts at:      0     308     728    1509 
   Size:        308     420     781

  Fragments arranged by size: 

                 781     420     308
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1187    1217    1509 
   Size:       1187      30     292

  Fragments arranged by size: 

                1187     292      30
 
BseYI C'CCAG_C

Cuts at:      0      62     608     668    1509 
   Size:         62     546      60     841

  Fragments arranged by size: 

                 841     546      62      60
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     464    1438    1509 
   Size:         91     373     974      71

  Fragments arranged by size: 

                 974     373      91      71
 
BsiHKAI G_wGCw'C

Cuts at:      0    1323    1509 
   Size:       1323     186
 
Bsp1286I G_dGCh'C

Cuts at:      0    1323    1509 
   Size:       1323     186
 
BspEI T'CCGG_A

Cuts at:      0     308     728    1509 
   Size:        308     420     781

  Fragments arranged by size: 

                 781     420     308
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      66     211    1410    1509 
   Size:         66     145    1199      99

  Fragments arranged by size: 

                1199     145      99      66
 
BssSI C'ACGA_G

Cuts at:      0     371    1509 
   Size:        371    1138
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     108    1509 
   Size:        108    1401
 
BstEII G'GTnAC_C

Cuts at:      0    1020    1386    1509 
   Size:       1020     366     123

  Fragments arranged by size: 

                1020     366     123
 
BstYI r'GATC_y

Cuts at:      0    1358    1509 
   Size:       1358     151
 
BtgI C'CryG_G

Cuts at:      0    1016    1049    1427    1469    1509 
   Size:       1016      33     378      42      40

  Fragments arranged by size: 

                1016     378      42      40      33
 
BtsI GCAGTG_nn'

Cuts at:      0      62    1172    1509 
   Size:         62    1110     337

  Fragments arranged by size: 

                1110     337      62
 
DraI TTT'AAA

Cuts at:      0     432    1509 
   Size:        432    1077
 
DraIII CAC_nnn'GTG

Cuts at:      0      15    1509 
   Size:         15    1494
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1462    1509 
   Size:       1462      47
 
EaeI y'GGCC_r

Cuts at:      0     236    1424    1476    1509 
   Size:        236    1188      52      33

  Fragments arranged by size: 

                1188     236      52      33
 
EarI CTCTTCn'nnn_

Cuts at:      0     307     743    1509 
   Size:        307     436     766

  Fragments arranged by size: 

                 766     436     307
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520     725     986    1349    1509 
   Size:        520     205     261     363     160

  Fragments arranged by size: 

                 520     363     261     205     160
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1255    1399    1509 
   Size:       1255     144     110

  Fragments arranged by size: 

                1255     144     110
 
EcoO109I rG'GnC_Cy

Cuts at:      0     223    1509 
   Size:        223    1286
 
EcoRI G'AATT_C

Cuts at:      0     575    1509 
   Size:        575     934
 
EcoRV GAT'ATC

Cuts at:      0     711    1451    1509 
   Size:        711     740      58

  Fragments arranged by size: 

                 740     711      58
 
HaeII r_GCGC'y

Cuts at:      0    1216    1509 
   Size:       1216     293
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     112     144     192     224     487     519    1187    1219 
   Size:        112      32      48      32     263      32     668      32

Cuts at:   1219    1271    1303    1509 
   Size:         52      32     206

  Fragments arranged by size: 

                 668     263     206     112      52      48      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     543    1509 
   Size:        543     966
 
HpaI GTT'AAC

Cuts at:      0     543    1509 
   Size:        543     966
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1296    1509 
   Size:       1296     213
 
MscI TGG'CCA

Cuts at:      0    1426    1509 
   Size:       1426      83
 
MspA1I CmG'CkG

Cuts at:      0     190     217     241    1509 
   Size:        190      27      24    1268

  Fragments arranged by size: 

                1268     190      27      24
 
NcoI C'CATG_G

Cuts at:      0    1427    1469    1509 
   Size:       1427      42      40

  Fragments arranged by size: 

                1427      42      40
 
NdeI CA'TA_TG

Cuts at:      0      32    1509 
   Size:         32    1477
 
NspI r_CATG'y

Cuts at:      0      55    1374    1509 
   Size:         55    1319     135

  Fragments arranged by size: 

                1319     135      55
 
PasI CC'CwG_GG

Cuts at:      0     716    1401    1509 
   Size:        716     685     108

  Fragments arranged by size: 

                 716     685     108
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     698    1143    1383    1485    1509 
   Size:        698     445     240     102      24

  Fragments arranged by size: 

                 698     445     240     102      24
 
PfoI T'CCnGG_A

Cuts at:      0     488    1509 
   Size:        488    1021
 
PpuMI rG'GwC_Cy

Cuts at:      0     223    1509 
   Size:        223    1286
 
PshAI GACnn'nnGTC

Cuts at:      0     916    1509 
   Size:        916     593
 
PsiI TTA'TAA

Cuts at:      0     809    1509 
   Size:        809     700
 
PstI C_TGCA'G

Cuts at:      0     222    1509 
   Size:        222    1287
 
PvuII CAG'CTG

Cuts at:      0     190     217    1509 
   Size:        190      27    1292

  Fragments arranged by size: 

                1292     190      27
 
SapI GCTCTTCn'nnn_

Cuts at:      0     743    1509 
   Size:        743     766
 
ScaI AGT'ACT

Cuts at:      0     643    1509 
   Size:        643     866
 
SfcI C'TryA_G

Cuts at:      0     218     647     914    1199    1509 
   Size:        218     429     267     285     310

  Fragments arranged by size: 

                 429     310     285     267     218
 
SmlI C'TyrA_G

Cuts at:      0     637    1509 
   Size:        637     872
 
StyI C'CwwG_G

Cuts at:      0    1427    1469    1509 
   Size:       1427      42      40

  Fragments arranged by size: 

                1427      42      40
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1150    1509 
   Size:       1150     359
 
TatI w'GTAC_w

Cuts at:      0     473     641    1509 
   Size:        473     168     868

  Fragments arranged by size: 

                 868     473     168
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1140    1342    1509 
   Size:       1140     202     167

  Fragments arranged by size: 

                1140     202     167
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AhdI    AlwNI     ApoI     AvaI     BbsI
    BbvCI     BglI     BplI     BpmI   Bpu10I    BpuEI    BsaBI    BsaWI
    BsaXI    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspMI    BssSI
   BstAPI   BstEII    BstYI     BtgI     BtsI     DraI   DraIII     DrdI
     EaeI     EarI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV    HaeII
    Hin4I   HincII     HpaI     MmeI     MscI   MspA1I     NcoI     NdeI
     NspI     PasI    PflMI     PfoI    PpuMI    PshAI     PsiI     PstI
    PvuII     SapI     ScaI     SfcI     SmlI     StyI    TaqII     TatI
     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI     BcgI    BciVI     BclI
    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BsaI
    BsaAI    BsaHI    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BstBI    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     EagI     EciI  EcoICRI     FalI
     FseI     FspI    FspAI  HindIII     KasI     KpnI     MfeI     MluI
     MslI     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI     PmeI     PmlI     PpiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI
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