Restriction map for pF1KB7878
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AvaI        AviII       
AvrII       BalI        BanI        BanII       BbeI        
BbrPI       BclI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsiEI       BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BsrBI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      CelII       CfoI        ClaI        
CpoI        DraIII      DrdI        DsaI        EaeI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      KasI        KpnI        KspI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        MvnI        NarI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI         1805  GTCTAC               GT^[AC][GT]AC
AcsI         1831  GAATTT               [GA]^AATT[CT]
AflIII        653  ACATGT               A^C[GA][CT]GT
AhdI          436  GACTCACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI        1811  CAGTTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI         1831  GAATTT               [GA]^AATT[CT]
AspEI         436  GACTCACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          595  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BcgI          100  GCAAACAGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI         1728  CCGGG                CC^[GC]GG
BglII          90  AGATCT               A^GATCT
BsaI         1350  GAGACC               GGTCTC(1/5)
BsgI          803  GTGCAG               GTGCAG(16/14)
BspLU11I      653  ACATGT               A^CATGT
BspMI         790  ACCTGC               ACCTGC(4/8)
BsrDI        1496  GCAATG               GCAATG(2/0)
BsrFI         743  GCCGGC               [GA]^CCGG[CT]
BstBI          50  TTCGAA               TT^CGAA
BstXI        1354  CCATCTGAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        743  GCCGGC               [GA]^CCGG[CT]
DraI          874  TTTAAA               TTT^AAA
Eam1105I      436  GACTCACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I      1570  CCAAGG               C^C[AT][AT]GG
FauI          862  CCCGC                CCCGC(4/6)
HincII       1837  GTTAAC               GT[CT]^[GA]AC
HindII       1837  GTTAAC               GT[CT]^[GA]AC
HindIII      1774  AAGCTT               A^AGCTT
HpaI         1837  GTTAAC               GTT^AAC
NaeI          743  GCCGGC               GCC^GGC
NciI         1728  CCGGG                CC^[GC]GG
NgoMIV        743  GCCGGC               G^CCGGC
NspI          653  ACATGT               [GA]CATG^[CT]
NspV           50  TTCGAA               TT^CGAA
PflFI         595  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        1810  CCAGTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI          997  GAAGAGC              GCTCTTC(1/4)
SfuI           50  TTCGAA               TT^CGAA
StuI         1881  AGGCCT               AGG^CCT
StyI         1570  CCAAGG               C^C[AT][AT]GG
TaqI           51  TCGA                 T^CGA
Tth111I       595  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I        51  TCGA                 T^CGA
Van91I       1810  CCAGTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          912  CCAGGAAGGTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AfaI          965  GTAC                 GT^AC
             1732  GTAC                
AspHI          84  GAGCAC               G[AT]GC[AT]^C
             1000  GAGCAC              
BamHI          27  GGATCC               G^GATCC
              111  GGATCC              
BbvCI           4  CCTCAGC              CCTCAGC(-5/-2)
              577  GCTGAGG             
BciVI         273  GGATAC               GTATCC(6/5)
             1898  GGATAC              
BmyI           84  GAGCAC               G[AGT]GC[ACT]^C
             1000  GAGCAC              
BsiHKAI        84  GAGCAC               G[AT]GC[AT]^C
             1000  GAGCAC              
BsmFI         583  GTCCC                GGGAC(10/14)
              902  GTCCC               
Bsp1286I       84  GAGCAC               G[AGT]GC[ACT]^C
             1000  GAGCAC              
HapII         744  CCGG                 C^CGG
             1728  CCGG                
HpaII         744  CCGG                 C^CGG
             1728  CCGG                
MslI          766  CACACAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1355  CATCTGAGTG          
MspA1I        315  CAGCTG               C[AC]G^C[GT]G
              542  CAGCTG              
MspI          744  CCGG                 C^CGG
             1728  CCGG                
MwoI          856  GCCACTCCCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1600  GCTTCCAGAGC         
PshAI        1536  GACTCCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1568  GACCAAGGTC          
PstI          571  CTGCAG               CTGCA^G
             1859  CTGCAG              
PvuII         315  CAGCTG               CAG^CTG
              542  CAGCTG              
RsaI          965  GTAC                 GT^AC
             1732  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (   60 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BbsI        
BbvI        BfaI        BpmI        BpuAI       BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EarI        
EcoNI       EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MvaI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com