Restriction map for pF1KB7878
 (Linear) (Six-base) MAPSORT of: KIBB7878.seq  Check: 7886  from: 1  to: 1917



KIBB7878.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     799    1917 
   Size:        799    1118
 
AccI GT'mk_AC

Cuts at:      0    1806    1917 
   Size:       1806     111
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1082    1270    1917 
   Size:       1082     188     647

  Fragments arranged by size: 

                1082     647     188
 
AflIII A'CryG_T

Cuts at:      0     653    1917 
   Size:        653    1264
 
AhdI GACnn_n'nnGTC

Cuts at:      0     441    1917 
   Size:        441    1476
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1816    1917 
   Size:       1816     101
 
ApoI r'AATT_y

Cuts at:      0    1831    1917 
   Size:       1831      86
 
BamHI G'GATC_C

Cuts at:      0      27     111    1917 
   Size:         27      84    1806

  Fragments arranged by size: 

                1806      84      27
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     293     652    1250    1463    1861    1917 
   Size:        293     359     598     213     398      56

  Fragments arranged by size: 

                 598     398     359     293     213      56
 
BbvCI CC'TCA_GC

Cuts at:      0       5     578    1917 
   Size:          5     573    1339

  Fragments arranged by size: 

                1339     573       5
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      89     123    1917 
   Size:         89      34    1794

  Fragments arranged by size: 

                1794      89      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     267    1892    1917 
   Size:        267    1625      25

  Fragments arranged by size: 

                1625     267      25
 
BglII A'GATC_T

Cuts at:      0      90    1917 
   Size:         90    1827
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     270     724    1626    1917 
   Size:        270     454     902     291

  Fragments arranged by size: 

                 902     454     291     270
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232    1471    1523    1898    1917 
   Size:        232    1239      52     375      19

  Fragments arranged by size: 

                1239     375     232      52      19
 
Bpu10I CC'TnA_GC

Cuts at:      0       5     578    1917 
   Size:          5     573    1339

  Fragments arranged by size: 

                1339     573       5
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1344    1917 
   Size:       1344     573
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1871    1901    1917 
   Size:       1871      30      16

  Fragments arranged by size: 

                1871      30      16
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1087    1096    1162    1165    1474    1917 
   Size:       1087       9      66       3     309     443

  Fragments arranged by size: 

                1087     443     309      66       9       3
 
BseYI C'CCAG_C

Cuts at:      0    1816    1917 
   Size:       1816     101
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     824    1917 
   Size:        824    1093
 
BsiHKAI G_wGCw'C

Cuts at:      0      88    1004    1917 
   Size:         88     916     913

  Fragments arranged by size: 

                 916     913      88
 
Bsp1286I G_dGCh'C

Cuts at:      0      88    1004    1917 
   Size:         88     916     913

  Fragments arranged by size: 

                 916     913      88
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     799    1917 
   Size:        799    1118
 
BsrDI GCAATG_nn'

Cuts at:      0    1503    1917 
   Size:       1503     414
 
BsrFI r'CCGG_y

Cuts at:      0     743    1917 
   Size:        743    1174
 
BstBI TT'CG_AA

Cuts at:      0      51    1917 
   Size:         51    1866
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1361    1917 
   Size:       1361     556
 
BstYI r'GATC_y

Cuts at:      0      27      90     111    1917 
   Size:         27      63      21    1806

  Fragments arranged by size: 

                1806      63      27      21
 
BtsI GCAGTG_nn'

Cuts at:      0     812    1128    1917 
   Size:        812     316     789

  Fragments arranged by size: 

                 812     789     316
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     323     358    1917 
   Size:        323      35    1559

  Fragments arranged by size: 

                1559     323      35
 
DraI TTT'AAA

Cuts at:      0     876    1917 
   Size:        876    1041
 
EarI CTCTTCn'nnn_

Cuts at:      0     383     513     848     992    1154    1160    1273    1917 
   Size:        383     130     335     144     162       6     113     644

  Fragments arranged by size: 

                 644     383     335     162     144     130     113       6
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      20    1917 
   Size:         20    1897
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232    1082    1270    1471    1523    1898    1917 
   Size:        232     850     188     201      52     375      19

  Fragments arranged by size: 

                 850     375     232     201     188      52      19
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     845     910    1252    1917 
   Size:        845      65     342     665

  Fragments arranged by size: 

                 845     665     342      65
 
EcoO109I rG'GnC_Cy

Cuts at:      0     582     919    1662    1756    1917 
   Size:        582     337     743      94     161

  Fragments arranged by size: 

                 743     582     337     161      94
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     693    1917 
   Size:        693    1224
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      75     107     427     459     825     857     936     968 
   Size:         75      32     320      32     366      32      79      32

Cuts at:    968    1195    1227    1455    1487    1917 
   Size:        227      32     228      32     430

  Fragments arranged by size: 

                 430     366     320     228     227      79      75      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1839    1917 
   Size:       1839      78
 
HindIII A'AGCT_T

Cuts at:      0    1774    1917 
   Size:       1774     143
 
HpaI GTT'AAC

Cuts at:      0    1839    1917 
   Size:       1839      78
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     433    1049    1917 
   Size:        433     616     868

  Fragments arranged by size: 

                 868     616     433
 
MslI CAynn'nnrTG

Cuts at:      0     770    1359    1917 
   Size:        770     589     558

  Fragments arranged by size: 

                 770     589     558
 
MspA1I CmG'CkG

Cuts at:      0     317     544    1917 
   Size:        317     227    1373

  Fragments arranged by size: 

                1373     317     227
 
NaeI GCC'GGC

Cuts at:      0     745    1917 
   Size:        745    1172
 
NgoMIV G'CCGG_C

Cuts at:      0     743    1917 
   Size:        743    1174
 
NspI r_CATG'y

Cuts at:      0     657    1917 
   Size:        657    1260
 
PciI A'CATG_T

Cuts at:      0     653    1917 
   Size:        653    1264
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1816    1917 
   Size:       1816     101
 
PfoI T'CCnGG_A

Cuts at:      0     230    1917 
   Size:        230    1687
 
PpuMI rG'GwC_Cy

Cuts at:      0     582     919    1756    1917 
   Size:        582     337     837     161

  Fragments arranged by size: 

                 837     582     337     161
 
PshAI GACnn'nnGTC

Cuts at:      0    1540    1572    1917 
   Size:       1540      32     345

  Fragments arranged by size: 

                1540     345      32
 
PsiI TTA'TAA

Cuts at:      0     397    1917 
   Size:        397    1520
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     778     810    1917 
   Size:        778      32    1107

  Fragments arranged by size: 

                1107     778      32
 
PstI C_TGCA'G

Cuts at:      0     575    1863    1917 
   Size:        575    1288      54

  Fragments arranged by size: 

                1288     575      54
 
PvuII CAG'CTG

Cuts at:      0     317     544    1917 
   Size:        317     227    1373

  Fragments arranged by size: 

                1373     317     227
 
SapI GCTCTTCn'nnn_

Cuts at:      0     992    1917 
   Size:        992     925
 
SfcI C'TryA_G

Cuts at:      0     571    1238    1859    1917 
   Size:        571     667     621      58

  Fragments arranged by size: 

                 667     621     571      58
 
StuI AGG'CCT

Cuts at:      0    1883    1917 
   Size:       1883      34
 
StyI C'CwwG_G

Cuts at:      0    1570    1917 
   Size:       1570     347
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1024    1792    1917 
   Size:       1024     768     125

  Fragments arranged by size: 

                1024     768     125
 
TatI w'GTAC_w

Cuts at:      0     964    1917 
   Size:        964     953
 
Tth111I GACn'n_nGTC

Cuts at:      0     598    1917 
   Size:        598    1319
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     919    1917 
   Size:        919     998
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AhdI    AlwNI     ApoI    BamHI
     BbsI    BbvCI     BcgI    BciVI    BglII     BmrI     BpmI   Bpu10I
     BsaI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI
    BsrDI    BsrFI    BstBI    BstXI    BstYI     BtsI    CspCI     DraI
     EarI     EciI  Eco57MI    EcoNI EcoO109I     FalI    Hin4I   HincII
  HindIII     HpaI     MmeI     MslI   MspA1I     NaeI   NgoMIV     NspI
     PciI    PflMI     PfoI    PpuMI    PshAI     PsiI     PsrI     PstI
    PvuII     SapI     SfcI     StuI     StyI    TaqII     TatI  Tth111I
     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AleI     AlfI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI     BanI    BanII     BbeI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrGI   BssHII    BssSI   BstAPI   BstEII  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI   DraIII     DrdI     EaeI     EagI  EcoICRI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII     KasI     KpnI     MfeI
     MluI     MscI     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PmeI     PmlI     PpiI   PspOMI    PspXI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TstI     XbaI     XhoI
     XmaI     XmnI     ZraI
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