Restriction map for pF1KB7224
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        AlwNI       Aor51HI     ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvrII       BamHI       BanI        BbeI        
BbrPI       BbsI        BcgI        BciVI       BfaI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      Bst1107I    
BstBI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoRI       EcoRV       
EcoT22I     FseI        HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MfeI        MluI        MroI        MseI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsaI        RsrII       
SacI        SacII       SalI        SapI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        SspBI       
SspI        StuI        SwaI        Tru9I       Tsp509I     
Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Alw44I        308  GTGCAC               G^TGCAC
AocI          753  CCTGAGG              CC^T[ACGT]AGG
ApaLI         308  GTGCAC               G^TGCAC
AvaI          566  CCCGGG               C^[CT]CG[GA]G
AviII         274  TGCGCA               TGC^GCA
BalI          162  TGGCCA               TGG^CCA
BclI          737  TGATCA               T^GATCA
BglI          505  GCCGTCCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          630  CTCCAG               CTGGAG(16/14)
BsaI            4  GGTCTC               GGTCTC(1/5)
BsgI          388  CTGCAC               GTGCAG(16/14)
BsoBI         566  CCCGGG               C^[CT]CG[GA]G
BssSI         727  CTCGTG               CACGAG(-5/-1)
BstEII         24  GGTCACC              G^GT[ACGT]ACC
BstPI          24  GGTCACC              G^GT[ACGT]ACC
BstXI         446  CCACAACAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1005  AGATCC               [GA]^GATC[CT]
Bsu36I        753  CCTGAGG              CC^T[ACGT]AGG
DrdI          249  GACTCTGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          162  TGGCCA               [CT]^GGCC[GA]
EarI          595  GAAGAG               CTCTTC(1/4)
Eco81I        753  CCTGAGG              CC^T[ACGT]AGG
EcoO65I        24  GGTCACC              G^GT[ACGT]ACC
FauI          147  GCGGG                CCCGC(4/6)
FbaI          737  TGATCA               T^GATCA
FspI          274  TGCGCA               TGC^GCA
Ksp632I       595  GAAGAG               CTCTTC(1/4)
MaeII         829  ACGT                 A^CGT
MboII         595  GAAGA                GAAGA(8/7)
MflI         1005  AGATCC               [GA]^GATC[CT]
MluNI         162  TGGCCA               TGG^CCA
MscI          162  TGGCCA               TGG^CCA
PflMI         666  CCATACCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          249  GACTC                GAGTC(4/5)
PstI           30  CTGCAG               CTGCA^G
PvuII         717  CAGCTG               CAG^CTG
SbfI           29  CCTGCAGG             CCTGCA^GG
SfaNI          73  GCATC                GCATC(5/9)
SmaI          566  CCCGGG               CCC^GGG
Sse8387I       29  CCTGCAGG             CCTGCA^GG
TaqI          354  TCGA                 T^CGA
TthHB8I       354  TCGA                 T^CGA
Van91I        666  CCATACCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          323  CCAAAGGCTCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1005  AGATCC               [GA]^GATC[CT]
XmaCI         566  CCCGGG               C^CCGGG
XmaI          566  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccII         146  CGCG                 CG^CG
              916  CGCG                
AcyI          136  GACGCC               G[GA]^CG[CT]C
              229  GACGCC              
AlwI         1006  GATCC                GGATC(4/5)
              570  GGATC               
ApaI          347  GGGCCC               GGGCC^C
              575  GGGCCC              
AspHI         308  GTGCAC               G[AT]GC[AT]^C
              458  GTGCTC              
BsaHI         136  GACGCC               G[GA]^CG[CT]C
              229  GACGCC              
BsiHKAI       308  GTGCAC               G[AT]GC[AT]^C
              458  GTGCTC              
BsmAI         786  GAGAC                GTCTC(1/5)
                5  GTCTC               
BstAPI        266  GCAGCTTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1029  GCACTGTATGC         
BstUI         146  CGCG                 CG^CG
              916  CGCG                
DsaI          332  CCATGG               C^C[GA][CT]GG
              671  CCATGG              
EcoNI         364  CCTCCTTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              753  CCTGAGGGAGG         
HaeII         513  GGCGCT               [GA]GCGC^[CT]
              732  GGCGCT              
Hin1I         136  GACGCC               G[GA]^CG[CT]C
              229  GACGCC              
MslI          393  CACCAGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              447  CACAACAGTG          
MvnI          146  CGCG                 CG^CG
              916  CGCG                
NcoI          332  CCATGG               C^CATGG
              671  CCATGG              
PpuMI          34  AGGTCCC              [GA]G^G[AT]CC[CT]
              466  AGGTCCC             
SfcI           30  CTGCAG               C^T[GA][CT]AG
              472  CTACAG              


#########################################
# 3 OR MORE CUT ENZYMES (   58 enzymes) #
#########################################

AciI        AluI        AvaII       BanII       BbvCI       
BbvI        BcnI        BmyI        BsaJI       BseRI       
BsiYI       BslI        BsmFI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MnlI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       StyI        TfiI        
TseI        Tsp45I      TspRI       
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