Restriction map for pF1KB7224
 (Linear) (Six-base) MAPSORT of: KIBB7224.seq  Check: 7343  from: 1  to: 1068



KIBB7224.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      37     174    1068 
   Size:         37     137     894

  Fragments arranged by size: 

                 894     137      37
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     353     893    1068 
   Size:        353     540     175

  Fragments arranged by size: 

                 540     353     175
 
AleI CACnn'nnGTG

Cuts at:      0     451    1068 
   Size:        451     617
 
ApaI G_GGCC'C

Cuts at:      0     351     579    1068 
   Size:        351     228     489

  Fragments arranged by size: 

                 489     351     228
 
ApaLI G'TGCA_C

Cuts at:      0     308    1068 
   Size:        308     760
 
AvaI C'yCGr_G

Cuts at:      0     566    1068 
   Size:        566     502
 
BanII G_rGCy'C

Cuts at:      0     351     437     579    1068 
   Size:        351      86     142     489

  Fragments arranged by size: 

                 489     351     142      86
 
BbvCI CC'TCA_GC

Cuts at:      0     193     517     715     865    1068 
   Size:        193     324     198     150     203

  Fragments arranged by size: 

                 324     203     198     193     150
 
BclI T'GATC_A

Cuts at:      0     737    1068 
   Size:        737     331
 
BglI GCCn_nnn'nGGC

Cuts at:      0     511    1068 
   Size:        511     557
 
Bme1580I G_kGCm'C

Cuts at:      0     312     351     579     899    1068 
   Size:        312      39     228     320     169

  Fragments arranged by size: 

                 320     312     228     169      39
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     645    1068 
   Size:        645     423
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     615    1068 
   Size:        615     453
 
Bpu10I CC'TnA_GC

Cuts at:      0     193     517     715     865    1068 
   Size:        193     324     198     150     203

  Fragments arranged by size: 

                 324     203     198     193     150
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      10    1068 
   Size:         10    1058
 
BsaHI Gr'CG_yC

Cuts at:      0     137     230    1068 
   Size:        137      93     838

  Fragments arranged by size: 

                 838     137      93
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      84     114     399     429    1068 
   Size:         84      30     285      30     639

  Fragments arranged by size: 

                 639     285      84      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     431     775     947    1068 
   Size:        431     344     172     121

  Fragments arranged by size: 

                 431     344     172     121
 
BseYI C'CCAG_C

Cuts at:      0      51     530    1068 
   Size:         51     479     538

  Fragments arranged by size: 

                 538     479      51
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     373    1068 
   Size:        373     695
 
BsiHKAI G_wGCw'C

Cuts at:      0     312     462    1068 
   Size:        312     150     606

  Fragments arranged by size: 

                 606     312     150
 
Bsp1286I G_dGCh'C

Cuts at:      0     312     351     437     462     579     899    1068 
   Size:        312      39      86      25     117     320     169

  Fragments arranged by size: 

                 320     312     169     117      86      39      25
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      23      37     174    1068 
   Size:         23      14     137     894

  Fragments arranged by size: 

                 894     137      23      14
 
BssSI C'ACGA_G

Cuts at:      0     727    1068 
   Size:        727     341
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     272    1035    1068 
   Size:        272     763      33

  Fragments arranged by size: 

                 763     272      33
 
BstEII G'GTnAC_C

Cuts at:      0      24    1068 
   Size:         24    1044
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     453    1068 
   Size:        453     615
 
BstYI r'GATC_y

Cuts at:      0    1005    1068 
   Size:       1005      63
 
Bsu36I CC'TnA_GG

Cuts at:      0     754    1068 
   Size:        754     314
 
BtgI C'CryG_G

Cuts at:      0     332     671    1068 
   Size:        332     339     397

  Fragments arranged by size: 

                 397     339     332
 
BtsI GCAGTG_nn'

Cuts at:      0     498    1068 
   Size:        498     570
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     255    1068 
   Size:        255     813
 
EaeI y'GGCC_r

Cuts at:      0     162    1068 
   Size:        162     906
 
EarI CTCTTCn'nnn_

Cuts at:      0     590    1068 
   Size:        590     478
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     787     940    1068 
   Size:        787     153     128

  Fragments arranged by size: 

                 787     153     128
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     353     615     893    1068 
   Size:        353     262     278     175

  Fragments arranged by size: 

                 353     278     262     175
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     368     757    1068 
   Size:        368     389     311

  Fragments arranged by size: 

                 389     368     311
 
EcoO109I rG'GnC_Cy

Cuts at:      0      35     347     348     467    1068 
   Size:         35     312       1     119     601

  Fragments arranged by size: 

                 601     312     119      35       1
 
FspI TGC'GCA

Cuts at:      0     276    1068 
   Size:        276     792
 
HaeII r_GCGC'y

Cuts at:      0     517     736    1068 
   Size:        517     219     332

  Fragments arranged by size: 

                 517     332     219
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     181     213     399     431     809     841    1068 
   Size:        181      32     186      32     378      32     227

  Fragments arranged by size: 

                 378     227     186     181      32      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1022    1068 
   Size:       1022      46
 
MscI TGG'CCA

Cuts at:      0     164    1068 
   Size:        164     904
 
MslI CAynn'nnrTG

Cuts at:      0     397     451    1068 
   Size:        397      54     617

  Fragments arranged by size: 

                 617     397      54
 
MspA1I CmG'CkG

Cuts at:      0     653     690     719    1068 
   Size:        653      37      29     349

  Fragments arranged by size: 

                 653     349      37      29
 
NcoI C'CATG_G

Cuts at:      0     332     671    1068 
   Size:        332     339     397

  Fragments arranged by size: 

                 397     339     332
 
PasI CC'CwG_GG

Cuts at:      0     382    1068 
   Size:        382     686
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     672    1068 
   Size:        672     396
 
PfoI T'CCnGG_A

Cuts at:      0     224    1068 
   Size:        224     844
 
PpuMI rG'GwC_Cy

Cuts at:      0      35     467    1068 
   Size:         35     432     601

  Fragments arranged by size: 

                 601     432      35
 
PspOMI G'GGCC_C

Cuts at:      0     347     575    1068 
   Size:        347     228     493

  Fragments arranged by size: 

                 493     347     228
 
PstI C_TGCA'G

Cuts at:      0      34    1068 
   Size:         34    1034
 
PvuII CAG'CTG

Cuts at:      0     719    1068 
   Size:        719     349
 
SbfI CC_TGCA'GG

Cuts at:      0      34    1068 
   Size:         34    1034
 
SfcI C'TryA_G

Cuts at:      0      30     472    1068 
   Size:         30     442     596

  Fragments arranged by size: 

                 596     442      30
 
SmaI CCC'GGG

Cuts at:      0     568    1068 
   Size:        568     500
 
StyI C'CwwG_G

Cuts at:      0     233     332     671     978    1009    1068 
   Size:        233      99     339     307      31      59

  Fragments arranged by size: 

                 339     307     233      99      59      31
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     445    1068 
   Size:        445     623
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     330    1068 
   Size:        330     738
 
XmaI C'CCGG_G

Cuts at:      0     566    1068 
   Size:        566     502
 

 Enzymes that do cut:

     AarI     AcuI     AleI     ApaI    ApaLI     AvaI    BanII    BbvCI
     BclI     BglI Bme1580I     BplI     BpmI   Bpu10I     BsaI    BsaHI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BssSI
   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI     DrdI
     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I     FspI    HaeII
    Hin4I     MmeI     MscI     MslI   MspA1I     NcoI     PasI    PflMI
     PfoI    PpuMI   PspOMI     PstI    PvuII     SbfI     SfcI     SmaI
     StyI    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI     BcgI    BciVI
    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI    BpuEI    BsaAI
    BsaBI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BstBI  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     EagI  EcoICRI    EcoRI    EcoRV
     FalI     FseI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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