Restriction map for pF1KE0504
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AflII       AflIII      AhdI        
Alw44I      Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BseAI       BsiHKAI     
BsiWI       BsmFI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrGI       BsrI        BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     CelII       ClaI        
CpoI        DraI        DraIII      DrdI        Eam1105I    
Eco47III    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HpaI        KasI        KpnI        KspI        MaeI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MslI        MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tsp509I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AcyI          411  GGCGTC               G[GA]^CG[CT]C
AgeI          257  ACCGGT               A^CCGGT
AlwI          479  GGATC                GGATC(4/5)
AlwNI         151  CAGCGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          376  CCTGAGG              CC^T[ACGT]AGG
AspI          276  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BcnI          353  CCCGG                CC^[GC]GG
BsaHI         411  GGCGTC               G[GA]^CG[CT]C
BsaI          458  GAGACC               GGTCTC(1/5)
BsaWI         257  ACCGGT               [AT]^CCGG[AT]
BsgI          286  GTGCAG               GTGCAG(16/14)
BsmBI         607  CGTCTC               CGTCTC(1/5)
BstYI         479  GGATCT               [GA]^GATC[CT]
Bsu36I        376  CCTGAGG              CC^T[ACGT]AGG
Cac8I         213  GCTGGC               GC[ACGT]^[ACGT]GC
DpnII         480  GATC                 ^GATC
DsaI          419  CCACGG               C^C[GA][CT]GG
EaeI           43  CGGCCG               [CT]^GGCC[GA]
EagI           43  CGGCCG               C^GGCCG
EclXI          43  CGGCCG               C^GGCCG
Eco52I         43  CGGCCG               C^GGCCG
Eco81I        376  CCTGAGG              CC^T[ACGT]AGG
EcoT14I       571  CCAAGG               C^C[AT][AT]GG
HgaI          412  GCGTC                GACGC(5/10)
Hin1I         411  GGCGTC               G[GA]^CG[CT]C
HindIII       175  AAGCTT               A^AGCTT
MboI          480  GATC                 ^GATC
MflI          479  GGATCT               [GA]^GATC[CT]
MseI          146  TTAA                 T^TAA
NciI          353  CCCGG                CC^[GC]GG
NdeII         480  GATC                 ^GATC
NlaIII        505  CATG                 CATG^
NspI          504  ACATGC               [GA]CATG^[CT]
PflFI         276  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         257  ACCGGT               A^CCGGT
Sau3AI        480  GATC                 ^GATC
StyI          571  CCAAGG               C^C[AT][AT]GG
Tru9I         146  TTAA                 T^TAA
TspRI         330  CAGTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       276  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          326  CCATCAGTGACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         479  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AfaI           87  GTAC                 GT^AC
              584  GTAC                
AvaI          345  CTCGGG               C^[CT]CG[GA]G
              361  CTCGGG              
AvaII         255  GGACC                G^G[AT]CC
              339  GGACC               
BpmI          235  CTGGAG               CTGGAG(16/14)
              389  CTGGAG              
BsiEI          36  CGACCG               CG[GA][CT]^CG
               43  CGGCCG              
BsoBI         345  CTCGGG               C^[CT]CG[GA]G
              361  CTCGGG              
BsrFI          38  ACCGGC               [GA]^CCGG[CT]
              257  ACCGGT              
BstF5I        416  CATCC                GGATG(2/0)
              538  CATCC               
CfoI          189  GCGC                 GCG^C
              600  GCGC                
Cfr10I         38  ACCGGC               [GA]^CCGG[CT]
              257  ACCGGT              
EarI           75  GAAGAG               CTCTTC(1/4)
              226  GAAGAG              
EcoNI         376  CCTGAGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              440  CCTTGCCCAGG         
FauI           81  GCGGG                CCCGC(4/6)
              632  GCGGG               
FokI          416  CATCC                GGATG(9/13)
              538  CATCC               
HaeII         188  AGCGCC               [GA]GCGC^[CT]
              599  AGCGCC              
HhaI          189  GCGC                 GCG^C
              600  GCGC                
HinP1I        189  GCGC                 G^CGC
              600  GCGC                
HinfI         280  GAGTC                G^A[ACGT]TC
              556  GACTC               
Ksp632I        75  GAAGAG               CTCTTC(1/4)
              226  GAAGAG              
PleI          556  GACTC                GAGTC(4/5)
              280  GAGTC               
RsaI           87  GTAC                 GT^AC
              584  GTAC                
SfaNI         210  GATGC                GCATC(5/9)
              496  GCATC               
TaqI           65  TCGA                 T^CGA
              116  TCGA                
TthHB8I        65  TCGA                 T^CGA
              116  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AciI        AluI        ApaI        BanII       BbvI        
BmyI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       Bsp1286I    BssKI       BstNI       Cfr13I      
DdeI        DraII       EcoO109I    EcoRII      Fnu4HI      
HaeIII      HapII       HpaII       HphI        ItaI        
MaeIII      MboII       MnlI        MspA1I      MspI        
MvaI        MwoI        NlaIV       Sau96I      ScrFI       
TseI        Tsp45I      
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com