Restriction map for pF1KE0504
 (Linear) (Six-base) MAPSORT of: KIEE0504.seq  Check: 1267  from: 1  to: 639



KIEE0504.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AgeI A'CCGG_T

Cuts at:      0     257     639 
   Size:        257     382
 
AlwNI CAG_nnn'CTG

Cuts at:      0     156     639 
   Size:        156     483
 
ApaI G_GGCC'C

Cuts at:      0     354     552     579     639 
   Size:        354     198      27      60

  Fragments arranged by size: 

                 354     198      60      27
 
AvaI C'yCGr_G

Cuts at:      0     345     361     639 
   Size:        345      16     278

  Fragments arranged by size: 

                 345     278      16
 
BanII G_rGCy'C

Cuts at:      0     354     552     579     639 
   Size:        354     198      27      60

  Fragments arranged by size: 

                 354     198      60      27
 
Bme1580I G_kGCm'C

Cuts at:      0     354     552     579     639 
   Size:        354     198      27      60

  Fragments arranged by size: 

                 354     198      60      27
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     410     639 
   Size:        256     154     229

  Fragments arranged by size: 

                 256     229     154
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     452     639 
   Size:        452     187
 
BsaHI Gr'CG_yC

Cuts at:      0     412     639 
   Size:        412     227
 
BsaWI w'CCGG_w

Cuts at:      0     257     639 
   Size:        257     382
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     397     427     639 
   Size:        397      30     212

  Fragments arranged by size: 

                 397     212      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      85     154     178     199     253     418     421     474 
   Size:         85      69      24      21      54     165       3      53

Cuts at:    474     639 
   Size:        165

  Fragments arranged by size: 

                 165     165      85      69      54      53      24      21
                   3
 
BseYI C'CCAG_C

Cuts at:      0     596     639 
   Size:        596      43
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     639 
   Size:        307     332
 
BsiEI CG_ry'CG

Cuts at:      0      39      46     639 
   Size:         39       7     593

  Fragments arranged by size: 

                 593      39       7
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     613     639 
   Size:        613      26
 
Bsp1286I G_dGCh'C

Cuts at:      0     354     552     579     639 
   Size:        354     198      27      60

  Fragments arranged by size: 

                 354     198      60      27
 
BsrFI r'CCGG_y

Cuts at:      0      38     257     639 
   Size:         38     219     382

  Fragments arranged by size: 

                 382     219      38
 
BstYI r'GATC_y

Cuts at:      0     479     639 
   Size:        479     160
 
Bsu36I CC'TnA_GG

Cuts at:      0     377     639 
   Size:        377     262
 
BtgI C'CryG_G

Cuts at:      0     419     639 
   Size:        419     220
 
EaeI y'GGCC_r

Cuts at:      0      43     639 
   Size:         43     596
 
EagI C'GGCC_G

Cuts at:      0      43     639 
   Size:         43     596
 
EarI CTCTTCn'nnn_

Cuts at:      0      70     221     639 
   Size:         70     151     418

  Fragments arranged by size: 

                 418     151      70
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     410     639 
   Size:        256     154     229

  Fragments arranged by size: 

                 256     229     154
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     380     444     639 
   Size:        380      64     195

  Fragments arranged by size: 

                 380     195      64
 
EcoO109I rG'GnC_Cy

Cuts at:      0     350     575     576     639 
   Size:        350     225       1      63

  Fragments arranged by size: 

                 350     225      63       1
 
HaeII r_GCGC'y

Cuts at:      0     192     603     639 
   Size:        192     411      36

  Fragments arranged by size: 

                 411     192      36
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     397     400     429     432     522     554     639 
   Size:        397       3      29       3      90      32      85

  Fragments arranged by size: 

                 397      90      85      32      29       3       3
 
HindIII A'AGCT_T

Cuts at:      0     175     639 
   Size:        175     464
 
MspA1I CmG'CkG

Cuts at:      0     153     477     632     639 
   Size:        153     324     155       7

  Fragments arranged by size: 

                 324     155     153       7
 
NspI r_CATG'y

Cuts at:      0     508     639 
   Size:        508     131
 
PspOMI G'GGCC_C

Cuts at:      0     350     548     575     639 
   Size:        350     198      27      64

  Fragments arranged by size: 

                 350     198      64      27
 
StyI C'CwwG_G

Cuts at:      0     571     639 
   Size:        571      68
 
Tth111I GACn'n_nGTC

Cuts at:      0     279     639 
   Size:        279     360
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     333     639 
   Size:        333     306
 

 Enzymes that do cut:

     AgeI    AlwNI     ApaI     AvaI    BanII Bme1580I     BpmI     BsaI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsmBI
 Bsp1286I    BsrFI    BstYI   Bsu36I     BtgI     EaeI     EagI     EarI
  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I  HindIII   MspA1I     NspI
   PspOMI     StyI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI
  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EciI
  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI     XbaI     XhoI     XmaI     XmnI     ZraI
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