Restriction map for pF1KB7318
 (Linear) (Six-base) MAPSORT of: KIBB7318.seq  Check: 682  from: 1  to: 3543



KIBB7318.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:07  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2297    2377    3543 
   Size:       2297      80    1166

  Fragments arranged by size: 

                2297    1166      80
 
AclI AA'CG_TT

Cuts at:      0     736    3543 
   Size:        736    2807
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     248     482    1546    2509    2614    3163    3229    3327 
   Size:        248     234    1064     963     105     549      66      98

Cuts at:   3327    3543 
   Size:        216

  Fragments arranged by size: 

                1064     963     549     248     234     216     105      98
                  66
 
AfeI AGC'GCT

Cuts at:      0    1531    3543 
   Size:       1531    2012
 
AflIII A'CryG_T

Cuts at:      0    1730    3543 
   Size:       1730    1813
 
AgeI A'CCGG_T

Cuts at:      0     761    3524    3543 
   Size:        761    2763      19

  Fragments arranged by size: 

                2763     761      19
 
AleI CACnn'nnGTG

Cuts at:      0    2808    3543 
   Size:       2808     735
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1988    3543 
   Size:       1988    1555
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1238    1270    3543 
   Size:       1238      32    2273

  Fragments arranged by size: 

                2273    1238      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1667    2094    2385    2724    2769    2895    3066    3543 
   Size:       1667     427     291     339      45     126     171     477

  Fragments arranged by size: 

                1667     477     427     339     291     171     126      45
            
 
ApoI r'AATT_y

Cuts at:      0     226     509     566     667    2147    3543 
   Size:        226     283      57     101    1480    1396

  Fragments arranged by size: 

                1480    1396     283     226     101      57
 
AvaI C'yCGr_G

Cuts at:      0    1478    1609    3126    3238    3479    3543 
   Size:       1478     131    1517     112     241      64

  Fragments arranged by size: 

                1517    1478     241     131     112      64
 
BanI G'GyrC_C

Cuts at:      0     592    1350    2275    2479    3543 
   Size:        592     758     925     204    1064

  Fragments arranged by size: 

                1064     925     758     592     204
 
BanII G_rGCy'C

Cuts at:      0     136    1048    1366    1625    3543 
   Size:        136     912     318     259    1918

  Fragments arranged by size: 

                1918     912     318     259     136
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     882    1300    1789    1877    2344    3011    3543 
   Size:        882     418     489      88     467     667     532

  Fragments arranged by size: 

                 882     667     532     489     467     418      88
 
BbvCI CC'TCA_GC

Cuts at:      0     674    1126    2192    2356    2775    3543 
   Size:        674     452    1066     164     419     768

  Fragments arranged by size: 

                1066     768     674     452     419     164
 
BglII A'GATC_T

Cuts at:      0    2597    3543 
   Size:       2597     946
 
Bme1580I G_kGCm'C

Cuts at:      0    1183    1355    3543 
   Size:       1183     172    2188

  Fragments arranged by size: 

                2188    1183     172
 
BmgBI CAC'GTC

Cuts at:      0    1176    1398    3392    3543 
   Size:       1176     222    1994     151

  Fragments arranged by size: 

                1994    1176     222     151
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1092    1953    2700    3543 
   Size:       1092     861     747     843

  Fragments arranged by size: 

                1092     861     843     747
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     966    3300    3543 
   Size:        966    2334     243

  Fragments arranged by size: 

                2334     966     243
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     936    1528    1687    1909    2107    2451    2752    3043 
   Size:        936     592     159     222     198     344     301     291

Cuts at:   3043    3088    3475    3543 
   Size:         45     387      68

  Fragments arranged by size: 

                 936     592     387     344     301     291     222     198
                 159      68      45
 
Bpu10I CC'TnA_GC

Cuts at:      0     130     674    1126    2192    2356    2775    2980    2986 
   Size:        130     544     452    1066     164     419     205       6

Cuts at:   2986    3543 
   Size:        557

  Fragments arranged by size: 

                1066     557     544     452     419     205     164     130
                   6
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1177    1288    2009    2715    3457    3543 
   Size:       1177     111     721     706     742      86

  Fragments arranged by size: 

                1177     742     721     706     111      86
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1953    3543 
   Size:       1953    1590
 
BsaAI yAC'GTr

Cuts at:      0      58     657    1731    3543 
   Size:         58     599    1074    1812

  Fragments arranged by size: 

                1812    1074     599      58
 
BsaHI Gr'CG_yC

Cuts at:      0    2000    3543 
   Size:       2000    1543
 
BsaWI w'CCGG_w

Cuts at:      0     761     978    2792    3524    3543 
   Size:        761     217    1814     732      19

  Fragments arranged by size: 

                1814     761     732     217      19
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     979    1009    1543    1573    3543 
   Size:        979      30     534      30    1970

  Fragments arranged by size: 

                1970     979     534      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     322     691     694    1687    2257    3144    3543 
   Size:        322     369       3     993     570     887     399

  Fragments arranged by size: 

                 993     887     570     399     369     322       3
 
BseYI C'CCAG_C

Cuts at:      0     848    1899    2623    3543 
   Size:        848    1051     724     920

  Fragments arranged by size: 

                1051     920     848     724
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     778    1429    1924    2440    3352    3484    3543 
   Size:        778     651     495     516     912     132      59

  Fragments arranged by size: 

                 912     778     651     516     495     132      59
 
BsiHKAI G_wGCw'C

Cuts at:      0      63     136    1625    1632    3543 
   Size:         63      73    1489       7    1911

  Fragments arranged by size: 

                1911    1489      73      63       7
 
BsmI GAATG_Cn'

Cuts at:      0     312    3504    3543 
   Size:        312    3192      39

  Fragments arranged by size: 

                3192     312      39
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1166    3543 
   Size:       1166    2377
 
Bsp1286I G_dGCh'C

Cuts at:      0      63     136    1048    1183    1355    1366    1625    1632 
   Size:         63      73     912     135     172      11     259       7

Cuts at:   1632    3543 
   Size:       1911

  Fragments arranged by size: 

                1911     912     259     172     135      73      63      11
                   7
 
BspHI T'CATG_A

Cuts at:      0    1316    3035    3543 
   Size:       1316    1719     508

  Fragments arranged by size: 

                1719    1316     508
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     410    2297    2377    3543 
   Size:        410    1887      80    1166

  Fragments arranged by size: 

                1887    1166     410      80
 
BsrBI CCG'CTC

Cuts at:      0    2328    3543 
   Size:       2328    1215
 
BsrDI GCAATG_nn'

Cuts at:      0     202    3543 
   Size:        202    3341
 
BsrFI r'CCGG_y

Cuts at:      0     761    3305    3524    3543 
   Size:        761    2544     219      19

  Fragments arranged by size: 

                2544     761     219      19
 
BssSI C'ACGA_G

Cuts at:      0    1861    3543 
   Size:       1861    1682
 
BstYI r'GATC_y

Cuts at:      0     456    2168    2461    2597    3153    3543 
   Size:        456    1712     293     136     556     390

  Fragments arranged by size: 

                1712     556     456     390     293     136
 
BtgI C'CryG_G

Cuts at:      0    1358    1796    2753    3543 
   Size:       1358     438     957     790

  Fragments arranged by size: 

                1358     957     790     438
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     341    3543 
   Size:        341    3202
 
BtsI GCAGTG_nn'

Cuts at:      0     707     939    2254    3543 
   Size:        707     232    1315    1289

  Fragments arranged by size: 

                1315    1289     707     232
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1816    1851    2053    2088    3543 
   Size:       1816      35     202      35    1455

  Fragments arranged by size: 

                1816    1455     202      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0      58    3543 
   Size:         58    3485
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1791    3543 
   Size:       1791    1752
 
EaeI y'GGCC_r

Cuts at:      0     721     961    2444    3543 
   Size:        721     240    1483    1099

  Fragments arranged by size: 

                1483    1099     721     240
 
EarI CTCTTCn'nnn_

Cuts at:      0    2489    3139    3543 
   Size:       2489     650     404

  Fragments arranged by size: 

                2489     650     404
 
EcoICRI GAG'CTC

Cuts at:      0     134    1623    3543 
   Size:        134    1489    1920

  Fragments arranged by size: 

                1920    1489     134
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     248     482     936    1528    1546    1687    1909    2107 
   Size:        248     234     454     592      18     141     222     198

Cuts at:   2107    2451    2509    2614    2752    3043    3088    3163    3229 
   Size:        344      58     105     138     291      45      75      66

Cuts at:   3229    3327    3475    3543 
   Size:         98     148      68

  Fragments arranged by size: 

                 592     454     344     291     248     234     222     198
                 148     141     138     105      98      75      68      66
                  58      45      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2239    3543 
   Size:       2239    1304
 
EcoO109I rG'GnC_Cy

Cuts at:      0      82    1134    1740    1893    3063    3543 
   Size:         82    1052     606     153    1170     480

  Fragments arranged by size: 

                1170    1052     606     480     153      82
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1866    2092    3543 
   Size:       1866     226    1451

  Fragments arranged by size: 

                1866    1451     226
 
HaeII r_GCGC'y

Cuts at:      0    1497    1533    3543 
   Size:       1497      36    2010

  Fragments arranged by size: 

                2010    1497      36
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     979    1011    1030    1062    1192    1224    1472    1504 
   Size:        979      32      19      32     130      32     248      32

Cuts at:   1504    2248    2280    2889    2921    3097    3129    3138    3170 
   Size:        744      32     609      32     176      32       9      32

Cuts at:   3170    3543 
   Size:        373

  Fragments arranged by size: 

                 979     744     609     373     248     176     130      32
                  32      32      32      32      32      32      32      19
                   9
 
HindIII A'AGCT_T

Cuts at:      0    1933    3019    3543 
   Size:       1933    1086     524

  Fragments arranged by size: 

                1933    1086     524
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2525    2843    3543 
   Size:       2525     318     700

  Fragments arranged by size: 

                2525     700     318
 
MscI TGG'CCA

Cuts at:      0     963    2446    3543 
   Size:        963    1483    1097

  Fragments arranged by size: 

                1483    1097     963
 
MslI CAynn'nnrTG

Cuts at:      0     629    1375    2808    3543 
   Size:        629     746    1433     735

  Fragments arranged by size: 

                1433     746     735     629
 
MspA1I CmG'CkG

Cuts at:      0     544     918    1389    2191    2298    3543 
   Size:        544     374     471     802     107    1245

  Fragments arranged by size: 

                1245     802     544     471     374     107
 
NcoI C'CATG_G

Cuts at:      0    1796    3543 
   Size:       1796    1747
 
NspI r_CATG'y

Cuts at:      0     281    2201    3254    3543 
   Size:        281    1920    1053     289

  Fragments arranged by size: 

                1920    1053     289     281
 
PasI CC'CwG_GG

Cuts at:      0    1917    3171    3543 
   Size:       1917    1254     372

  Fragments arranged by size: 

                1917    1254     372
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     959    2094    2769    3066    3543 
   Size:        959    1135     675     297     477

  Fragments arranged by size: 

                1135     959     675     477     297
 
PfoI T'CCnGG_A

Cuts at:      0    1703    3059    3543 
   Size:       1703    1356     484

  Fragments arranged by size: 

                1703    1356     484
 
PmlI CAC'GTG

Cuts at:      0      58     657    3543 
   Size:         58     599    2886

  Fragments arranged by size: 

                2886     599      58
 
PpuMI rG'GwC_Cy

Cuts at:      0      82    1134    1893    3063    3543 
   Size:         82    1052     759    1170     480

  Fragments arranged by size: 

                1170    1052     759     480      82
 
PspXI vC'TCGA_Gb

Cuts at:      0    1478    3543 
   Size:       1478    2065
 
PstI C_TGCA'G

Cuts at:      0     549    1388    1394    2720    2891    3543 
   Size:        549     839       6    1326     171     652

  Fragments arranged by size: 

                1326     839     652     549     171       6
 
PvuII CAG'CTG

Cuts at:      0     544    1389    2298    3543 
   Size:        544     845     909    1245

  Fragments arranged by size: 

                1245     909     845     544
 
SacI G_AGCT'C

Cuts at:      0     136    1625    3543 
   Size:        136    1489    1918

  Fragments arranged by size: 

                1918    1489     136
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3139    3543 
   Size:       3139     404
 
ScaI AGT'ACT

Cuts at:      0    1678    3543 
   Size:       1678    1865
 
SexAI A'CCwGG_T

Cuts at:      0    3326    3543 
   Size:       3326     217
 
SfcI C'TryA_G

Cuts at:      0     545    1384    1390    2716    2880    2887    3543 
   Size:        545     839       6    1326     164       7     656

  Fragments arranged by size: 

                1326     839     656     545     164       7       6
 
SmlI C'TyrA_G

Cuts at:      0    1192    1267    1478    2024    2694    3126    3472    3543 
   Size:       1192      75     211     546     670     432     346      71

  Fragments arranged by size: 

                1192     670     546     432     346     211      75      71
            
 
StuI AGG'CCT

Cuts at:      0    1442    3543 
   Size:       1442    2101
 
StyI C'CwwG_G

Cuts at:      0     480    1796    3287    3543 
   Size:        480    1316    1491     256

  Fragments arranged by size: 

                1491    1316     480     256
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2293    2297    2497    2546    3163    3543 
   Size:       2293       4     200      49     617     380

  Fragments arranged by size: 

                2293     617     380     200      49       4
 
TatI w'GTAC_w

Cuts at:      0    1676    1724    3543 
   Size:       1676      48    1819

  Fragments arranged by size: 

                1819    1676      48
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1543    1575    1877    1909    2499    2531    2642    2674 
   Size:       1543      32     302      32     590      32     111      32

Cuts at:   2674    2826    2858    3543 
   Size:        152      32     685

  Fragments arranged by size: 

                1543     685     590     302     152     111      32      32
                  32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2826    3324    3543 
   Size:       2826     498     219

  Fragments arranged by size: 

                2826     498     219
 
XhoI C'TCGA_G

Cuts at:      0    1478    3126    3543 
   Size:       1478    1648     417

  Fragments arranged by size: 

                1648    1478     417
 
XmnI GAAnn'nnTTC

Cuts at:      0     227    1531    1846    3340    3543 
   Size:        227    1304     315    1494     203

  Fragments arranged by size: 

                1494    1304     315     227     203
 

 Enzymes that do cut:

     AarI     AclI     AcuI     AfeI   AflIII     AgeI     AleI     AlfI
     AloI    AlwNI     ApoI     AvaI     BanI    BanII     BbsI    BbvCI
    BglII Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI    BstYI     BtgI    BtgZI     BtsI    CspCI   DraIII
     DrdI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
    HaeII    Hin4I  HindIII     MmeI     MscI     MslI   MspA1I     NcoI
     NspI     PasI    PflMI     PfoI     PmlI    PpuMI    PspXI     PstI
    PvuII     SacI     SapI     ScaI    SexAI     SfcI     SmlI     StuI
     StyI    TaqII     TatI     TstI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I    AflII     AhdI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI     BmtI    BsaBI    BsiEI    BsiWI
    BspEI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     ClaI     DraI     EagI     EciI    EcoRI    EcoRV     FseI
     FspI    FspAI   HincII     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI     PmeI     PpiI    PshAI     PsiI   PspOMI     PsrI
     PvuI    RsrII    SacII     SalI    SanDI     SbfI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI  Tth111I     XbaI     XmaI     ZraI
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