Restriction map for pF1KB8956
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

AccI        AccIII      AclI        AcsI        AflII       
AgeI        AhdI        AlwNI       AocI        ApoI        
AscI        AseI        AsnI        Asp700I     AspEI       
AspI        AvrII       BalI        BbvCI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BsaBI       BsaI        BsaWI       BseAI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrGI       BsrI        
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco52I      Eco81I      
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        HincII      HindII      HindIII     HpaI        
Ksp632I     MaeIII      MamI        MfeI        MluI        
MluNI       MroI        MscI        MslI        MunI        
NcoI        NdeI        NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmeI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SalI        SapI        
SbfI        ScaI        SexAI       SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tsp45I      
Tsp509I     Tth111I     Van91I      XbaI        XcmI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AatII          27  GACGTC               GACGT^C
Acc65I        226  GGTACC               G^GTACC
Alw44I        512  GTGCAC               G^TGCAC
ApaI          697  GGGCCC               GGGCC^C
ApaLI         512  GTGCAC               G^TGCAC
Asp718I       226  GGTACC               G^GTACC
AspHI         512  GTGCAC               G[AT]GC[AT]^C
AvaII         482  GGACC                G^G[AT]CC
AviII         976  TGCGCA               TGC^GCA
BamHI         361  GGATCC               G^GATCC
BbrPI         517  CACGTG               CAC^GTG
BbsI          652  GTCTTC               GAAGAC(2/6)
BfaI          412  CTAG                 C^TAG
BlpI          288  GCTCAGC              GC^T[ACGT]AGC
BpmI          661  CTGGAG               CTGGAG(16/14)
Bpu1102I      288  GCTCAGC              GC^T[ACGT]AGC
BpuAI         652  GTCTTC               GAAGAC(2/6)
BsaAI         517  CACGTG               [CT]AC^GT[GA]
BsgI          531  GTGCAG               GTGCAG(16/14)
BsiEI         468  CGACCG               CG[GA][CT]^CG
BsiHKAI       512  GTGCAC               G[AT]GC[AT]^C
BssHII        705  GCGCGC               G^CGCGC
BstF5I         39  CATCC                GGATG(2/0)
BstXI         830  CCAAGAAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         361  GGATCC               [GA]^GATC[CT]
CelII         288  GCTCAGC              GC^T[ACGT]AGC
EcoNI         411  CCTAGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       478  CCAAGG               C^C[AT][AT]GG
FokI           39  CATCC                GGATG(9/13)
FseI          345  GGCCGGCC             GGCCGG^CC
FspI          976  TGCGCA               TGC^GCA
KpnI          226  GGTACC               GGTAC^C
MaeI          412  CTAG                 C^TAG
MboII         653  TCTTC                GAAGA(8/7)
MflI          361  GGATCC               [GA]^GATC[CT]
MseI          674  TTAA                 T^TAA
PmaCI         517  CACGTG               CAC^GTG
PmlI          517  CACGTG               CAC^GTG
PstI          919  CTGCAG               CTGCA^G
SfaNI         121  GCATC                GCATC(5/9)
SfcI          919  CTGCAG               C^T[GA][CT]AG
SfiI          336  GGCCAGCGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI          106  CCCGGG               CCC^GGG
SmlI          366  CTTGAG               C^T[CT][GA]AG
StyI          478  CCAAGG               C^C[AT][AT]GG
TaqI          656  TCGA                 T^CGA
Tru9I         674  TTAA                 T^TAA
TspRI         917  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       656  TCGA                 T^CGA
XhoII         361  GGATCC               [GA]^GATC[CT]
XmaCI         106  CCCGGG               C^CCGGG
XmaI          106  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AfaI          227  GTAC                 GT^AC
              942  GTAC                
AflIII        516  ACACGT               A^C[GA][CT]GT
              518  ACGTGT              
Aor51HI       620  AGCGCT               AGC^GCT
              683  AGCGCT              
AvaI          106  CCCGGG               C^[CT]CG[GA]G
              700  CCCGAG              
BanII         258  GAGCCC               G[GA]GC[CT]^C
              697  GGGCCC              
BsmFI         173  GGGAC                GGGAC(10/14)
              272  GGGAC               
BsoBI         106  CCCGGG               C^[CT]CG[GA]G
              700  CCCGAG              
DdeI          289  CTCAG                C^T[ACGT]AG
              462  CTCAG               
DpnII          48  GATC                 ^GATC
              362  GATC                
DsaI          131  CCGCGG               C^C[GA][CT]GG
              425  CCGCGG              
Eco47III      620  AGCGCT               AGC^GCT
              683  AGCGCT              
HphI          747  GGTGA                GGTGA(8/7)
              734  TCACC               
KspI          131  CCGCGG               CCGC^GG
              425  CCGCGG              
MaeII          28  ACGT                 A^CGT
              518  ACGT                
MboI           48  GATC                 ^GATC
              362  GATC                
NdeII          48  GATC                 ^GATC
              362  GATC                
PvuII         236  CAGCTG               CAG^CTG
              980  CAGCTG              
RsaI          227  GTAC                 GT^AC
              942  GTAC                
SacII         131  CCGCGG               CCGC^GG
              425  CCGCGG              
Sau3AI         48  GATC                 ^GATC
              362  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
BanI        BbeI        BbvI        BcnI        BglI        
BmyI        BsaHI       BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmBI       Bsp1286I    BspMI       
BsrFI       BssKI       BstNI       BstUI       Cac8I       
CfoI        Cfr10I      Cfr13I      EaeI        EcoRII      
FauI        Fnu4HI      HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       ItaI        KasI        MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NgoMIV      NlaIV       PleI        
Sau96I      ScrFI       SfoI        TseI        
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