Restriction map for pF1KB8956
 (Linear) (Six-base) MAPSORT of: KIBB8956.seq  Check: 2241  from: 1  to: 999



KIBB8956.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     735     982     999 
   Size:        735     247      17

  Fragments arranged by size: 

                 735     247      17
 
AatII G_ACGT'C

Cuts at:      0      31     999 
   Size:         31     968
 
Acc65I G'GTAC_C

Cuts at:      0     226     999 
   Size:        226     773
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     748     999 
   Size:        748     251
 
AfeI AGC'GCT

Cuts at:      0     622     685     999 
   Size:        622      63     314

  Fragments arranged by size: 

                 622     314      63
 
AflIII A'CryG_T

Cuts at:      0     516     518     999 
   Size:        516       2     481

  Fragments arranged by size: 

                 516     481       2
 
ApaI G_GGCC'C

Cuts at:      0     701     999 
   Size:        701     298
 
ApaLI G'TGCA_C

Cuts at:      0     512     999 
   Size:        512     487
 
AvaI C'yCGr_G

Cuts at:      0     106     700     999 
   Size:        106     594     299

  Fragments arranged by size: 

                 594     299     106
 
BamHI G'GATC_C

Cuts at:      0     361     999 
   Size:        361     638
 
BanI G'GyrC_C

Cuts at:      0      13      95     102     193     226     600     819     991 
   Size:         13      82       7      91      33     374     219     172

Cuts at:    991     999 
   Size:          8

  Fragments arranged by size: 

                 374     219     172      91      82      33      13       8
                   7
 
BanII G_rGCy'C

Cuts at:      0     262     701     999 
   Size:        262     439     298

  Fragments arranged by size: 

                 439     298     262
 
BbeI G_GCGC'C

Cuts at:      0      17      99     106     197     604     823     999 
   Size:         17      82       7      91     407     219     176

  Fragments arranged by size: 

                 407     219     176      91      82      17       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     645     999 
   Size:        645     354
 
BglI GCCn_nnn'nGGC

Cuts at:      0      93     266     343     956     999 
   Size:         93     173      77     613      43

  Fragments arranged by size: 

                 613     173      93      77      43
 
BlpI GC'TnA_GC

Cuts at:      0     289     999 
   Size:        289     710
 
Bme1580I G_kGCm'C

Cuts at:      0     115     516     701     999 
   Size:        115     401     185     298

  Fragments arranged by size: 

                 401     298     185     115
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     682     999 
   Size:        682     317
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     387     999 
   Size:        387     612
 
BsaAI yAC'GTr

Cuts at:      0     519     999 
   Size:        519     480
 
BsaHI Gr'CG_yC

Cuts at:      0      14      28      96     103     194     214     572     601 
   Size:         14      14      68       7      91      20     358      29

Cuts at:    601     820     999 
   Size:        219     179

  Fragments arranged by size: 

                 358     219     179      91      68      29      20      14
                  14       7
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     265     295     999 
   Size:        265      30     704

  Fragments arranged by size: 

                 704     265      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      79     310     430     595     598     601     604     999 
   Size:         79     231     120     165       3       3       3     395

  Fragments arranged by size: 

                 395     231     165     120      79       3       3       3
            
 
BseYI C'CCAG_C

Cuts at:      0     269     959     999 
   Size:        269     690      40

  Fragments arranged by size: 

                 690     269      40
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     552     999 
   Size:        552     447
 
BsiEI CG_ry'CG

Cuts at:      0     471     999 
   Size:        471     528
 
BsiHKAI G_wGCw'C

Cuts at:      0     516     999 
   Size:        516     483
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     221     465     733     999 
   Size:        221     244     268     266

  Fragments arranged by size: 

                 268     266     244     221
 
Bsp1286I G_dGCh'C

Cuts at:      0     115     262     516     701     999 
   Size:        115     147     254     185     298

  Fragments arranged by size: 

                 298     254     185     147     115
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     639     735     815     887     982     999 
   Size:        639      96      80      72      95      17

  Fragments arranged by size: 

                 639      96      95      80      72      17
 
BsrFI r'CCGG_y

Cuts at:      0     223     346     381     541     564     594     788     999 
   Size:        223     123      35     160      23      30     194     211

  Fragments arranged by size: 

                 223     211     194     160     123      35      30      23
            
 
BssHII G'CGCG_C

Cuts at:      0     705     999 
   Size:        705     294
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     837     999 
   Size:        837     162
 
BstYI r'GATC_y

Cuts at:      0     361     999 
   Size:        361     638
 
BtgI C'CryG_G

Cuts at:      0     131     425     999 
   Size:        131     294     574

  Fragments arranged by size: 

                 574     294     131
 
BtsI GCAGTG_nn'

Cuts at:      0     916     999 
   Size:        916      83
 
EaeI y'GGCC_r

Cuts at:      0      80     128     375     395     716     839     898     999 
   Size:         80      48     247      20     321     123      59     101

  Fragments arranged by size: 

                 321     247     123     101      80      59      48      20
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     441     999 
   Size:        441     558
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     682     748     999 
   Size:        682      66     251

  Fragments arranged by size: 

                 682     251      66
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     415     999 
   Size:        415     584
 
FseI GG_CCGG'CC

Cuts at:      0     350     999 
   Size:        350     649
 
FspI TGC'GCA

Cuts at:      0     978     999 
   Size:        978      21
 
HaeII r_GCGC'y

Cuts at:      0      17      99     106     197     318     604     624     669 
   Size:         17      82       7      91     121     286      20      45

Cuts at:    669     687     789     823     967     999 
   Size:         18     102      34     144      32

  Fragments arranged by size: 

                 286     144     121     102      91      82      45      34
                  32      20      18      17       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     265     297     999 
   Size:        265      32     702

  Fragments arranged by size: 

                 702     265      32
 
KasI G'GCGC_C

Cuts at:      0      13      95     102     193     600     819     999 
   Size:         13      82       7      91     407     219     180

  Fragments arranged by size: 

                 407     219     180      91      82      13       7
 
KpnI G_GTAC'C

Cuts at:      0     230     999 
   Size:        230     769
 
MspA1I CmG'CkG

Cuts at:      0     118     133     142     238     341     427     536     560 
   Size:        118      15       9      96     103      86     109      24

Cuts at:    560     982     999 
   Size:        422      17

  Fragments arranged by size: 

                 422     118     109     103      96      86      24      17
                  15       9
 
NaeI GCC'GGC

Cuts at:      0     348     543     566     596     790     999 
   Size:        348     195      23      30     194     209

  Fragments arranged by size: 

                 348     209     195     194      30      23
 
NarI GG'CG_CC

Cuts at:      0      14      96     103     194     601     820     999 
   Size:         14      82       7      91     407     219     179

  Fragments arranged by size: 

                 407     219     179      91      82      14       7
 
NgoMIV G'CCGG_C

Cuts at:      0     346     541     564     594     788     999 
   Size:        346     195      23      30     194     211

  Fragments arranged by size: 

                 346     211     195     194      30      23
 
PmlI CAC'GTG

Cuts at:      0     519     999 
   Size:        519     480
 
PspOMI G'GGCC_C

Cuts at:      0     697     999 
   Size:        697     302
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     757     789     999 
   Size:        757      32     210

  Fragments arranged by size: 

                 757     210      32
 
PstI C_TGCA'G

Cuts at:      0     923     999 
   Size:        923      76
 
PvuII CAG'CTG

Cuts at:      0     238     982     999 
   Size:        238     744      17

  Fragments arranged by size: 

                 744     238      17
 
SacII CC_GC'GG

Cuts at:      0     134     428     999 
   Size:        134     294     571

  Fragments arranged by size: 

                 571     294     134
 
SfcI C'TryA_G

Cuts at:      0     919     999 
   Size:        919      80
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     343     999 
   Size:        343     656
 
SfoI GGC'GCC

Cuts at:      0      15      97     104     195     602     821     999 
   Size:         15      82       7      91     407     219     178

  Fragments arranged by size: 

                 407     219     178      91      82      15       7
 
SmaI CCC'GGG

Cuts at:      0     108     999 
   Size:        108     891
 
SmlI C'TyrA_G

Cuts at:      0     366     999 
   Size:        366     633
 
StyI C'CwwG_G

Cuts at:      0     478     999 
   Size:        478     521
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     499     999 
   Size:        499     500
 
XmaI C'CCGG_G

Cuts at:      0     106     999 
   Size:        106     893
 
ZraI GAC'GTC

Cuts at:      0      29     999 
   Size:         29     970
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI     AfeI   AflIII     ApaI    ApaLI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BglI     BlpI
 Bme1580I     BpmI    BpuEI    BsaAI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrFI   BssHII
    BstXI    BstYI     BtgI     BtsI     EaeI     EciI  Eco57MI    EcoNI
     FseI     FspI    HaeII    Hin4I     KasI     KpnI   MspA1I     NaeI
     NarI   NgoMIV     PmlI   PspOMI     PsrI     PstI    PvuII    SacII
     SfcI     SfiI     SfoI     SmaI     SmlI     StyI    TaqII     XmaI
     ZraI

 Enzymes that do not cut: 

     AccI     AclI    AflII     AgeI     AhdI     AleI     AlfI     AloI
    AlwNI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI     BmrI     BmtI
     BplI   Bpu10I     BsaI    BsaBI    BsaWI    BsiWI     BsmI    BspEI
    BspHI    BsrBI    BsrDI    BsrGI    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI    FspAI
   HincII  HindIII     HpaI     MfeI     MluI     MmeI     MscI     MslI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PvuI    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmnI
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