Restriction map for
pF1KB6482
(Linear) (Six-base) MAPSORT of: KIBB6482.seq Check: 3203 from: 1 to: 1068
KIBB6482.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 1068
Size: 424 644
AleI CACnn'nnGTG
Cuts at: 0 422 1068
Size: 422 646
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 859 1068
Size: 859 209
ApaI G_GGCC'C
Cuts at: 0 124 496 1068
Size: 124 372 572
Fragments arranged by size:
572 372 124
ApaLI G'TGCA_C
Cuts at: 0 881 1068
Size: 881 187
AvaI C'yCGr_G
Cuts at: 0 123 901 1068
Size: 123 778 167
Fragments arranged by size:
778 167 123
AvrII C'CTAG_G
Cuts at: 0 276 1068
Size: 276 792
BanI G'GyrC_C
Cuts at: 0 349 455 1068
Size: 349 106 613
Fragments arranged by size:
613 349 106
BanII G_rGCy'C
Cuts at: 0 67 124 141 496 713 730 894 902
Size: 67 57 17 355 217 17 164 8
Cuts at: 902 1068
Size: 166
Fragments arranged by size:
355 217 166 164 67 57 17 17
8
BbeI G_GCGC'C
Cuts at: 0 353 459 1068
Size: 353 106 609
Fragments arranged by size:
609 353 106
BbsI GAAGACnn'nnnn_
Cuts at: 0 141 1068
Size: 141 927
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 813 847 1068
Size: 813 34 221
Fragments arranged by size:
813 221 34
Bme1580I G_kGCm'C
Cuts at: 0 124 417 429 435 496 885 1068
Size: 124 293 12 6 61 389 183
Fragments arranged by size:
389 293 183 124 61 12 6
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 707 790 1068
Size: 619 88 83 278
Fragments arranged by size:
619 278 88 83
Bpu10I CC'TnA_GC
Cuts at: 0 488 896 1068
Size: 488 408 172
Fragments arranged by size:
488 408 172
BsaAI yAC'GTr
Cuts at: 0 636 716 1068
Size: 636 80 352
Fragments arranged by size:
636 352 80
BsaHI Gr'CG_yC
Cuts at: 0 350 456 1068
Size: 350 106 612
Fragments arranged by size:
612 350 106
BsaWI w'CCGG_w
Cuts at: 0 75 705 1068
Size: 75 630 363
Fragments arranged by size:
630 363 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 322 352 700 730 1068
Size: 322 30 348 30 338
Fragments arranged by size:
348 338 322 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 583 592 865 1068
Size: 583 9 273 203
Fragments arranged by size:
583 273 203 9
BseYI C'CCAG_C
Cuts at: 0 4 1029 1068
Size: 4 1025 39
Fragments arranged by size:
1025 39 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 277 376 1068
Size: 169 108 99 692
Fragments arranged by size:
692 169 108 99
BsiEI CG_ry'CG
Cuts at: 0 245 723 1068
Size: 245 478 345
Fragments arranged by size:
478 345 245
BsiHKAI G_wGCw'C
Cuts at: 0 885 1068
Size: 885 183
BsmBI CGTCTCn'nnnn_
Cuts at: 0 953 1068
Size: 953 115
Bsp1286I G_dGCh'C
Cuts at: 0 67 124 141 417 429 435 496 713
Size: 67 57 17 276 12 6 61 217
Cuts at: 713 730 885 894 902 1068
Size: 17 155 9 8 166
Fragments arranged by size:
276 217 166 155 67 61 57 17
17 12 9 8 6
BspEI T'CCGG_A
Cuts at: 0 75 1068
Size: 75 993
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 272 1068
Size: 272 796
BsrFI r'CCGG_y
Cuts at: 0 244 1068
Size: 244 824
Bsu36I CC'TnA_GG
Cuts at: 0 781 1068
Size: 781 287
BtgI C'CryG_G
Cuts at: 0 323 1068
Size: 323 745
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 983 1068
Size: 983 85
BtsI GCAGTG_nn'
Cuts at: 0 181 749 843 1068
Size: 181 568 94 225
Fragments arranged by size:
568 225 181 94
EaeI y'GGCC_r
Cuts at: 0 242 638 720 819 1068
Size: 242 396 82 99 249
Fragments arranged by size:
396 249 242 99 82
EagI C'GGCC_G
Cuts at: 0 242 720 1068
Size: 242 478 348
Fragments arranged by size:
478 348 242
EarI CTCTTCn'nnn_
Cuts at: 0 560 1049 1068
Size: 560 489 19
Fragments arranged by size:
560 489 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 382 1068
Size: 382 686
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 619 707 790 1068
Size: 424 195 88 83 278
Fragments arranged by size:
424 278 195 88 83
EcoNI CCTnn'n_nnAGG
Cuts at: 0 487 1068
Size: 487 581
EcoO109I rG'GnC_Cy
Cuts at: 0 97 492 493 1068
Size: 97 395 1 575
Fragments arranged by size:
575 395 97 1
HaeII r_GCGC'y
Cuts at: 0 353 391 459 1068
Size: 353 38 68 609
Fragments arranged by size:
609 353 68 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 904 936 1068
Size: 904 32 132
Fragments arranged by size:
904 132 32
KasI G'GCGC_C
Cuts at: 0 349 455 1068
Size: 349 106 613
Fragments arranged by size:
613 349 106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 1068
Size: 572 496
MslI CAynn'nnrTG
Cuts at: 0 206 422 653 1068
Size: 206 216 231 415
Fragments arranged by size:
415 231 216 206
MspA1I CmG'CkG
Cuts at: 0 56 85 597 956 1068
Size: 56 29 512 359 112
Fragments arranged by size:
512 359 112 56 29
NaeI GCC'GGC
Cuts at: 0 246 1068
Size: 246 822
NarI GG'CG_CC
Cuts at: 0 350 456 1068
Size: 350 106 612
Fragments arranged by size:
612 350 106
NgoMIV G'CCGG_C
Cuts at: 0 244 1068
Size: 244 824
NspI r_CATG'y
Cuts at: 0 813 1068
Size: 813 255
PasI CC'CwG_GG
Cuts at: 0 69 461 1068
Size: 69 392 607
Fragments arranged by size:
607 392 69
PfoI T'CCnGG_A
Cuts at: 0 218 1068
Size: 218 850
PmlI CAC'GTG
Cuts at: 0 636 716 1068
Size: 636 80 352
Fragments arranged by size:
636 352 80
PpuMI rG'GwC_Cy
Cuts at: 0 97 1068
Size: 97 971
PspOMI G'GGCC_C
Cuts at: 0 120 492 1068
Size: 120 372 576
Fragments arranged by size:
576 372 120
PstI C_TGCA'G
Cuts at: 0 269 596 934 1068
Size: 269 327 338 134
Fragments arranged by size:
338 327 269 134
PvuII CAG'CTG
Cuts at: 0 597 1068
Size: 597 471
SfcI C'TryA_G
Cuts at: 0 265 592 930 1068
Size: 265 327 338 138
Fragments arranged by size:
338 327 265 138
SfoI GGC'GCC
Cuts at: 0 351 457 1068
Size: 351 106 611
Fragments arranged by size:
611 351 106
SmaI CCC'GGG
Cuts at: 0 125 1068
Size: 125 943
StyI C'CwwG_G
Cuts at: 0 276 1068
Size: 276 792
TatI w'GTAC_w
Cuts at: 0 254 1068
Size: 254 814
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 700 732 1068
Size: 700 32 336
Fragments arranged by size:
700 336 32
XmaI C'CCGG_G
Cuts at: 0 123 1068
Size: 123 945
Enzymes that do cut:
AcuI AleI AlfI ApaI ApaLI AvaI AvrII BanI
BanII BbeI BbsI BcgI Bme1580I BpmI Bpu10I BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrFI Bsu36I BtgI BtgZI
BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PfoI PmlI PpuMI PspOMI PstI
PvuII SfcI SfoI SmaI StyI TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI AlwNI ApoI AscI AseI AsiSI
BaeI BamHI BbvCI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI
BsiWI BsmI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I ClaI CspCI
DraI DraIII DrdI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
MscI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI
XcmI XhoI XmnI ZraI