Restriction map for pF1KB5411
##################################
# NO CUT ENZYMES (  111 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AflII       
AflIII      AgeI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspI        AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BcgI        BciVI       BclI        
BfrI        BlnI        BsaBI       BsaI        BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspLU11I    
BsrDI       BsrFI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HpaI        KasI        KpnI        KspI        MamI        
MluI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
ScaI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        SspBI       SspI        
SwaI        Tth111I     Van91I      XbaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccII        1384  CGCG                 CG^CG
AcsI          295  AAATTT               [GA]^AATT[CT]
AcyI           88  GACGCC               G[GA]^CG[CT]C
AhdI         1507  GACAGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          403  CCTGAGG              CC^T[ACGT]AGG
ApoI          295  AAATTT               [GA]^AATT[CT]
Asp700I       499  GAATTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1507  GACAGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI          844  GTCTTC               GAAGAC(2/6)
BbvCI        1321  GCTGAGG              CCTCAGC(-5/-2)
BcnI            5  CCGGG                CC^[GC]GG
BfaI          989  CTAG                 C^TAG
BlpI         1213  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1213  GCTGAGC              GC^T[ACGT]AGC
BpuAI         844  GTCTTC               GAAGAC(2/6)
BsaAI        1437  CACGTA               [CT]AC^GT[GA]
BsaHI          88  GACGCC               G[GA]^CG[CT]C
BsgI         1681  CTGCAC               GTGCAG(16/14)
BsmFI        1758  GTCCC                GGGAC(10/14)
BsmI          309  GAATGC               GAATGC(1/-1)
BspHI         249  TCATGA               T^CATGA
BspMI        1370  ACCTGC               ACCTGC(4/8)
BsrBI        1003  GAGCGG               CCGCTC(-3/-3)
BssSI        1053  CACGAG               CACGAG(-5/-1)
BstUI        1384  CGCG                 CG^CG
Bsu36I        403  CCTGAGG              CC^T[ACGT]AGG
CelII        1213  GCTGAGC              GC^T[ACGT]AGC
Eam1105I     1507  GACAGCCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        403  CCTGAGG              CC^T[ACGT]AGG
HgaI           88  GACGC                GACGC(5/10)
Hin1I          88  GACGCC               G[GA]^CG[CT]C
MaeI          989  CTAG                 C^TAG
MfeI          105  CAATTG               C^AATTG
MslI          724  CACATTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          105  CAATTG               C^AATTG
MvnI         1384  CGCG                 CG^CG
NciI            5  CCGGG                CC^[GC]GG
PaeR7I       1019  CTCGAG               C^TCGAG
RcaI          249  TCATGA               T^CATGA
SacI         1780  GAGCTC               GAGCT^C
SbfI         1371  CCTGCAGG             CCTGCA^GG
SexAI         175  ACCTGGT              A^CC[AT]GGT
SfiI         1194  GGCCCAGAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I     1371  CCTGCAGG             CCTGCA^GG
StuI         1640  AGGCCT               AGG^CCT
XhoI         1019  CTCGAG               C^TCGAG
XmnI          499  GAATTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccIII        540  TCCGGA               T^CCGGA
              671  TCCGGA              
AlwI           74  GGATC                GGATC(4/5)
              246  GGATC               
AvaI         1019  CTCGAG               C^[CT]CG[GA]G
             1760  CCCGAG              
BalI          290  TGGCCA               TGG^CCA
             1106  TGGCCA              
BanII         163  GGGCTC               G[GA]GC[CT]^C
             1780  GAGCTC              
BglII         389  AGATCT               A^GATCT
              758  AGATCT              
BsaWI         540  TCCGGA               [AT]^CCGG[AT]
              671  TCCGGA              
BseAI         540  TCCGGA               T^CCGGA
              671  TCCGGA              
BsmAI        1135  GAGAC                GTCTC(1/5)
             1663  GAGAC               
BsoBI        1019  CTCGAG               C^[CT]CG[GA]G
             1760  CCCGAG              
BspEI         540  TCCGGA               T^CCGGA
              671  TCCGGA              
BstYI         389  AGATCT               [GA]^GATC[CT]
              758  AGATCT              
EcoT14I        83  CCATGG               C^C[AT][AT]GG
             1553  CCATGG              
HindIII       865  AAGCTT               A^AGCTT
             1488  AAGCTT              
MaeII          61  ACGT                 A^CGT
             1438  ACGT                
MflI          389  AGATCT               [GA]^GATC[CT]
              758  AGATCT              
MluNI         290  TGGCCA               TGG^CCA
             1106  TGGCCA              
MroI          540  TCCGGA               T^CCGGA
              671  TCCGGA              
MscI          290  TGGCCA               TGG^CCA
             1106  TGGCCA              
MspA1I       1139  CAGCTG               C[AC]G^C[GT]G
             1357  CAGCTG              
NcoI           83  CCATGG               C^CATGG
             1553  CCATGG              
PvuII        1139  CAGCTG               CAG^CTG
             1357  CAGCTG              
SapI         1397  GAAGAGC              GCTCTTC(1/4)
             1777  GAAGAGC             
StyI           83  CCATGG               C^C[AT][AT]GG
             1553  CCATGG              
XcmI          438  CCAGGCCAAGTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1754  CCAAGTCCCGAGTGG     
XhoII         389  AGATCT               [GA]^GATC[CT]
              758  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AspHI       
AvaII       BbvI        BglI        BmyI        BpmI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
Bsp1286I    BsrI        BssKI       BstAPI      BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeIII      MboI        MboII       MnlI        MseI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     
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