Restriction map for pF1KB5411
 (Linear) (Six-base) MAPSORT of: KIBB5411.seq  Check: 4662  from: 1  to: 1785



KIBB5411.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:25  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      15     335     643    1333    1381    1785 
   Size:         15     320     308     690      48     404

  Fragments arranged by size: 

                 690     404     320     308      48      15
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1512    1785 
   Size:       1512     273
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     709    1618    1785 
   Size:        709     909     167

  Fragments arranged by size: 

                 909     709     167
 
AlwNI CAG_nnn'CTG

Cuts at:      0     639    1213    1371    1785 
   Size:        639     574     158     414

  Fragments arranged by size: 

                 639     574     414     158
 
ApoI r'AATT_y

Cuts at:      0     295    1785 
   Size:        295    1490
 
AvaI C'yCGr_G

Cuts at:      0    1019    1760    1785 
   Size:       1019     741      25

  Fragments arranged by size: 

                1019     741      25
 
BanII G_rGCy'C

Cuts at:      0     167    1784    1785 
   Size:        167    1617       1

  Fragments arranged by size: 

                1617     167       1
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     837    1785 
   Size:        837     948
 
BbvCI CC'TCA_GC

Cuts at:      0    1322    1785 
   Size:       1322     463
 
BglI GCCn_nnn'nGGC

Cuts at:      0     439    1201    1210    1348    1785 
   Size:        439     762       9     138     437

  Fragments arranged by size: 

                 762     439     437     138       9
 
BglII A'GATC_T

Cuts at:      0     389     758    1785 
   Size:        389     369    1027

  Fragments arranged by size: 

                1027     389     369
 
BlpI GC'TnA_GC

Cuts at:      0    1214    1785 
   Size:       1214     571
 
Bme1580I G_kGCm'C

Cuts at:      0     688    1785 
   Size:        688    1097
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     458    1785 
   Size:        458    1327
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     661     874    1438    1785 
   Size:        661     213     564     347

  Fragments arranged by size: 

                 661     564     347     213
 
Bpu10I CC'TnA_GC

Cuts at:      0     632    1322    1785 
   Size:        632     690     463

  Fragments arranged by size: 

                 690     632     463
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      25    1609    1785 
   Size:         25    1584     176

  Fragments arranged by size: 

                1584     176      25
 
BsaAI yAC'GTr

Cuts at:      0    1439    1785 
   Size:       1439     346
 
BsaHI Gr'CG_yC

Cuts at:      0      89    1785 
   Size:         89    1696
 
BsaWI w'CCGG_w

Cuts at:      0     540     671    1785 
   Size:        540     131    1114

  Fragments arranged by size: 

                1114     540     131
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     334     568     849     939     994    1054    1086    1147 
   Size:        334     234     281      90      55      60      32      61

Cuts at:   1147    1189    1192    1273    1276    1339    1350    1785 
   Size:         42       3      81       3      63      11     435

  Fragments arranged by size: 

                 435     334     281     234      90      81      63      61
                  60      55      42      32      11       3       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1666    1785 
   Size:       1666     119
 
BsiHKAI G_wGCw'C

Cuts at:      0     419     584    1784    1785 
   Size:        419     165    1200       1

  Fragments arranged by size: 

                1200     419     165       1
 
BsmI GAATG_Cn'

Cuts at:      0     315    1785 
   Size:        315    1470
 
Bsp1286I G_dGCh'C

Cuts at:      0     167     419     584     688    1784    1785 
   Size:        167     252     165     104    1096       1

  Fragments arranged by size: 

                1096     252     167     165     104       1
 
BspEI T'CCGG_A

Cuts at:      0     540     671    1785 
   Size:        540     131    1114

  Fragments arranged by size: 

                1114     540     131
 
BspHI T'CATG_A

Cuts at:      0     249    1785 
   Size:        249    1536
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1379    1785 
   Size:       1379     406
 
BsrBI CCG'CTC

Cuts at:      0    1005    1785 
   Size:       1005     780
 
BssSI C'ACGA_G

Cuts at:      0    1053    1785 
   Size:       1053     732
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1224    1287    1371    1785 
   Size:       1224      63      84     414

  Fragments arranged by size: 

                1224     414      84      63
 
BstYI r'GATC_y

Cuts at:      0     389     758    1785 
   Size:        389     369    1027

  Fragments arranged by size: 

                1027     389     369
 
Bsu36I CC'TnA_GG

Cuts at:      0     404    1785 
   Size:        404    1381
 
BtgI C'CryG_G

Cuts at:      0      83     769    1553    1785 
   Size:         83     686     784     232

  Fragments arranged by size: 

                 784     686     232      83
 
EaeI y'GGCC_r

Cuts at:      0       2     290    1106    1785 
   Size:          2     288     816     679

  Fragments arranged by size: 

                 816     679     288       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     311    1392    1772    1785 
   Size:        311    1081     380      13

  Fragments arranged by size: 

                1081     380     311      13
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1405    1785 
   Size:       1405     380
 
EcoICRI GAG'CTC

Cuts at:      0    1782    1785 
   Size:       1782       3
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      15     335     643     661     874    1333    1381    1438 
   Size:         15     320     308      18     213     459      48      57

Cuts at:   1438    1785 
   Size:        347

  Fragments arranged by size: 

                 459     347     320     308     213      57      48      18
                  15
 
EcoO109I rG'GnC_Cy

Cuts at:      0     132     636    1368    1785 
   Size:        132     504     732     417

  Fragments arranged by size: 

                 732     504     417     132
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     153     780    1758    1785 
   Size:        153     627     978      27

  Fragments arranged by size: 

                 978     627     153      27
 
HaeII r_GCGC'y

Cuts at:      0    1014    1272    1754    1785 
   Size:       1014     258     482      31

  Fragments arranged by size: 

                1014     482     258      31
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1498    1530    1584    1616    1785 
   Size:       1498      32      54      32     169

  Fragments arranged by size: 

                1498     169      54      32      32
 
HindIII A'AGCT_T

Cuts at:      0     865    1488    1785 
   Size:        865     623     297

  Fragments arranged by size: 

                 865     623     297
 
MfeI C'AATT_G

Cuts at:      0     105    1785 
   Size:        105    1680
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1046    1721    1785 
   Size:       1046     675      64

  Fragments arranged by size: 

                1046     675      64
 
MscI TGG'CCA

Cuts at:      0     292    1108    1785 
   Size:        292     816     677

  Fragments arranged by size: 

                 816     677     292
 
MslI CAynn'nnrTG

Cuts at:      0     728    1785 
   Size:        728    1057
 
MspA1I CmG'CkG

Cuts at:      0    1141    1359    1785 
   Size:       1141     218     426

  Fragments arranged by size: 

                1141     426     218
 
NcoI C'CATG_G

Cuts at:      0      83    1553    1785 
   Size:         83    1470     232

  Fragments arranged by size: 

                1470     232      83
 
PasI CC'CwG_GG

Cuts at:      0     714    1785 
   Size:        714    1071
 
PfoI T'CCnGG_A

Cuts at:      0    1415    1785 
   Size:       1415     370
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      85     117    1785 
   Size:         85      32    1668

  Fragments arranged by size: 

                1668      85      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     132     636    1368    1785 
   Size:        132     504     732     417

  Fragments arranged by size: 

                 732     504     417     132
 
PstI C_TGCA'G

Cuts at:      0     194    1085    1376    1607    1785 
   Size:        194     891     291     231     178

  Fragments arranged by size: 

                 891     291     231     194     178
 
PvuII CAG'CTG

Cuts at:      0    1141    1359    1785 
   Size:       1141     218     426

  Fragments arranged by size: 

                1141     426     218
 
SacI G_AGCT'C

Cuts at:      0    1784    1785 
   Size:       1784       1
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1392    1772    1785 
   Size:       1392     380      13

  Fragments arranged by size: 

                1392     380      13
 
SbfI CC_TGCA'GG

Cuts at:      0    1376    1785 
   Size:       1376     409
 
SexAI A'CCwGG_T

Cuts at:      0     175    1785 
   Size:        175    1610
 
SfcI C'TryA_G

Cuts at:      0     190    1081    1372    1603    1785 
   Size:        190     891     291     231     182

  Fragments arranged by size: 

                 891     291     231     190     182
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1201    1785 
   Size:       1201     584
 
SmlI C'TyrA_G

Cuts at:      0      40    1019    1624    1785 
   Size:         40     979     605     161

  Fragments arranged by size: 

                 979     605     161      40
 
StuI AGG'CCT

Cuts at:      0    1642    1785 
   Size:       1642     143
 
StyI C'CwwG_G

Cuts at:      0      83    1553    1785 
   Size:         83    1470     232

  Fragments arranged by size: 

                1470     232      83
 
TatI w'GTAC_w

Cuts at:      0     194    1785 
   Size:        194    1591
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     445    1761    1785 
   Size:        445    1316      24

  Fragments arranged by size: 

                1316     445      24
 
XhoI C'TCGA_G

Cuts at:      0    1019    1785 
   Size:       1019     766
 
XmnI GAAnn'nnTTC

Cuts at:      0     503    1785 
   Size:        503    1282
 

 Enzymes that do cut:

     AcuI     AhdI     AlfI    AlwNI     ApoI     AvaI    BanII     BbsI
    BbvCI     BglI    BglII     BlpI Bme1580I     BmrI     BpmI   Bpu10I
    BpuEI    BsaAI    BsaHI    BsaWI    BseRI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BssSI   BstAPI    BstYI
   Bsu36I     BtgI     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
     FalI    HaeII    Hin4I  HindIII     MfeI     MmeI     MscI     MslI
   MspA1I     NcoI     PasI     PfoI     PpiI    PpuMI     PstI    PvuII
     SacI     SapI     SbfI    SexAI     SfcI     SfiI     SmlI     StuI
     StyI     TatI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI     BclI
    BfrBI    BmgBI     BmtI     BplI     BsaI    BsaBI    BsaXI    BseYI
    BsiEI    BsiWI    BsmBI    BsrDI    BsrFI    BsrGI   BssHII    BstBI
   BstEII    BstXI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI   HincII     HpaI     KasI     KpnI     MluI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     ScaI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI    TaqII     TstI  Tth111I     XbaI     XmaI     ZraI
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