(Linear) (Six-base) MAPSORT of: KIBB6577.seq  Check: 5514  from: 1  to: 1131



KIBB6577.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AccI GT'mk_AC

Cuts at:      0    1013    1131 
   Size:       1013     118
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     503     752    1131 
   Size:        503     249     379

  Fragments arranged by size: 

                 503     379     249
 
AlwNI CAG_nnn'CTG

Cuts at:      0     265    1131 
   Size:        265     866
 
ApoI r'AATT_y

Cuts at:      0      28     126     340    1033    1131 
   Size:         28      98     214     693      98

  Fragments arranged by size: 

                 693     214      98      98      28
 
BanI G'GyrC_C

Cuts at:      0    1074    1131 
   Size:       1074      57
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     210    1131 
   Size:        210     921
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     572    1131 
   Size:        572     559
 
BsiHKAI G_wGCw'C

Cuts at:      0     186    1131 
   Size:        186     945
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     313    1131 
   Size:        313     818
 
Bsp1286I G_dGCh'C

Cuts at:      0     186    1131 
   Size:        186     945
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     274     573    1131 
   Size:        274     299     558

  Fragments arranged by size: 

                 558     299     274
 
BstBI TT'CG_AA

Cuts at:      0     123    1131 
   Size:        123    1008
 
BstEII G'GTnAC_C

Cuts at:      0     505    1131 
   Size:        505     626
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     686    1131 
   Size:        686     445
 
Bsu36I CC'TnA_GG

Cuts at:      0     222    1131 
   Size:        222     909
 
BtgI C'CryG_G

Cuts at:      0    1043    1131 
   Size:       1043      88
 
BtsI GCAGTG_nn'

Cuts at:      0     423    1131 
   Size:        423     708
 
DraI TTT'AAA

Cuts at:      0     990    1131 
   Size:        990     141
 
DraIII CAC_nnn'GTG

Cuts at:      0     796    1131 
   Size:        796     335
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     503     752    1131 
   Size:        503     249     379

  Fragments arranged by size: 

                 503     379     249
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     237    1131 
   Size:        237     894
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      91     123     253     285     358     390     916     948 
   Size:         91      32     130      32      73      32     526      32

Cuts at:    948    1131 
   Size:        183

  Fragments arranged by size: 

                 526     183     130      91      73      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     294    1131 
   Size:        294     837
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     435    1020    1131 
   Size:         41     394     585     111

  Fragments arranged by size: 

                 585     394     111      41
 
NcoI C'CATG_G

Cuts at:      0    1043    1131 
   Size:       1043      88
 
NspI r_CATG'y

Cuts at:      0     327     580    1131 
   Size:        327     253     551

  Fragments arranged by size: 

                 551     327     253
 
PasI CC'CwG_GG

Cuts at:      0     298    1131 
   Size:        298     833
 
StuI AGG'CCT

Cuts at:      0     810    1131 
   Size:        810     321
 
StyI C'CwwG_G

Cuts at:      0     841    1043    1131 
   Size:        841     202      88

  Fragments arranged by size: 

                 841     202      88
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     413     493    1131 
   Size:        413      80     638

  Fragments arranged by size: 

                 638     413      80
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     165     197    1131 
   Size:        165      32     934

  Fragments arranged by size: 

                 934     165      32
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI     BanI    BciVI     BmrI  BsiHKAI
    BsmBI Bsp1286I   BstAPI    BstBI   BstEII    BstXI   Bsu36I     BtgI
     BtsI     DraI   DraIII  Eco57MI     FalI    Hin4I   HincII     MmeI
     NcoI     NspI     PasI     StuI     StyI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstYI  BstZ17I    BtgZI     ClaI    CspCI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TatI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI