Restriction map for pF1KE0169
##################################
# NO CUT ENZYMES (  166 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BlnI        BlpI        
BmyI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmI        Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DdeI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HindIII     HpaI        
HphI        KasI        KpnI        KspI        MaeII       
MamI        MfeI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MunI        MvnI        
NarI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tsp45I      Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AfaI          321  GTAC                 GT^AC
AlwI           78  GATCC                GGATC(4/5)
AlwNI         295  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       159  GAACTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          558  CCCGAG               C^[CT]CG[GA]G
BbsI          202  GTCTTC               GAAGAC(2/6)
BbvI           21  GCTGC                GCAGC(8/12)
BcnI           27  CCCGG                CC^[GC]GG
BfaI          364  CTAG                 C^TAG
BglII         452  AGATCT               A^GATCT
BpmI          289  CTGGAG               CTGGAG(16/14)
BpuAI         202  GTCTTC               GAAGAC(2/6)
BseRI         512  GAGGAG               GAGGAG(10/8)
BsgI          373  GTGCAG               GTGCAG(16/14)
BsmBI         479  GAGACG               CGTCTC(1/5)
BsmFI         232  GGGAC                GGGAC(10/14)
BsoBI         558  CCCGAG               C^[CT]CG[GA]G
BsrFI         568  GCCGGC               [GA]^CCGG[CT]
BstAPI         39  GCAGGACTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I        568  GCCGGC               [GA]^CCGG[CT]
Cfr13I        308  GGGCC                G^G[ACGT]CC
DsaI          494  CCATGG               C^C[GA][CT]GG
EaeI          570  CGGCCA               [CT]^GGCC[GA]
EcoRI         250  GAATTC               G^AATTC
EcoT14I       494  CCATGG               C^C[AT][AT]GG
Fnu4HI         21  GCTGC                GC^[ACGT]GC
HincII        426  GTCAAC               GT[CT]^[GA]AC
HindII        426  GTCAAC               GT[CT]^[GA]AC
HinfI         424  GAGTC                G^A[ACGT]TC
ItaI           21  GCTGC                GC^[ACGT]GC
MaeI          364  CTAG                 C^TAG
MaeIII        271  GTAAC                ^GT[ACGT]AC
MseI          143  TTAA                 T^TAA
NaeI          568  GCCGGC               GCC^GGC
NciI           27  CCCGG                CC^[GC]GG
NcoI          494  CCATGG               C^CATGG
NgoMIV        568  GCCGGC               G^CCGGC
NlaIV         514  GGAGCC               GG[ACGT]^[ACGT]CC
PleI          424  GAGTC                GAGTC(4/5)
PshAI         122  GACATTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          547  CTGCAG               CTGCA^G
RsaI          321  GTAC                 GT^AC
Sau96I        308  GGGCC                G^G[ACGT]CC
SfcI          547  CTGCAG               C^T[GA][CT]AG
StyI          494  CCATGG               C^C[AT][AT]GG
Tru9I         143  TTAA                 T^TAA
TseI           21  GCTGC                G^C[AT]GC
XmnI          159  GAACTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AciI          207  CCGC                 CCGC(-3/-1)
              459  CCGC                
AcsI          187  AAATTT               [GA]^AATT[CT]
              250  GAATTC              
ApoI          187  AAATTT               [GA]^AATT[CT]
              250  GAATTC              
BsaJI         494  CCATGG               C^C[ACGT][ACGT]GG
              510  CCGAGG              
BsiYI         168  CCCTTATGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              459  CCGCCAGATGG         
BslI          168  CCCTTATGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              459  CCGCCAGATGG         
BsmAI         107  GAGAC                GTCTC(1/5)
              479  GAGAC               
BsrI          116  CCAGT                ACTGG(1/-1)
              573  CCAGT               
BstF5I        507  CATCC                GGATG(2/0)
              177  GGATG               
BstNI          81  CCAGG                CC^[AT]GG
              327  CCTGG               
BstYI          77  AGATCC               [GA]^GATC[CT]
              452  AGATCT              
CfoI           24  GCGC                 GCG^C
              540  GCGC                
EarI          136  GAAGAG               CTCTTC(1/4)
              499  GAAGAG              
EcoRII         81  CCAGG                ^CC[AT]GG
              327  CCTGG               
FokI          507  CATCC                GGATG(9/13)
              177  GGATG               
HaeIII        309  GGCC                 GG^CC
              571  GGCC                
HapII          28  CCGG                 C^CGG
              569  CCGG                
HhaI           24  GCGC                 GCG^C
              540  GCGC                
HinP1I         24  GCGC                 G^CGC
              540  GCGC                
HpaII          28  CCGG                 C^CGG
              569  CCGG                
Ksp632I       136  GAAGAG               CTCTTC(1/4)
              499  GAAGAG              
MflI           77  AGATCC               [GA]^GATC[CT]
              452  AGATCT              
MspI           28  CCGG                 C^CGG
              569  CCGG                
MvaI           81  CCAGG                CC^[AT]GG
              327  CCTGG               
NspI          198  GCATGT               [GA]CATG^[CT]
              317  GCATGT              
SmlI          102  CTTGAG               C^T[CT][GA]AG
              264  CTTGAG              
TaqI          215  TCGA                 T^CGA
              277  TCGA                
Tsp509I       188  AATT                 ^AATT
              251  AATT                
TthHB8I       215  TCGA                 T^CGA
              277  TCGA                
XhoII          77  AGATCC               [GA]^GATC[CT]
              452  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AluI        BssKI       Cac8I       DpnII       MboI        
MboII       MnlI        MwoI        NdeII       NlaIII      
Sau3AI      ScrFI       SfaNI       TspRI       
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