Restriction map for pF1KB8889
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BamHI       
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BpuAI       BsaAI       BsaBI       BsaI        
BseAI       BseRI       BsgI        BsiWI       BsmAI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrDI       BsrGI       
BssHII      Bst1107I    BstAPI      BstBI       BstXI       
BstYI       BstZ17I     Bsu36I      ClaI        CpoI        
DpnII       DraI        DraIII      DrdI        Eam1105I    
EarI        Eco47III    Eco81I      EcoNI       EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HindIII     KpnI        Ksp632I     MaeI        MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MunI        NdeI        NdeII       
NheI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SalI        SapI        
Sau3AI      SbfI        ScaI        SexAI       SfaNI       
SfiI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tsp509I     Tth111I     XbaI        
XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII         397  GACGTC               GACGT^C
AfaI           84  GTAC                 GT^AC
AgeI          179  ACCGGT               A^CCGGT
AlwNI         415  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       216  GAACCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII         435  GAGCTC               G[GA]GC[CT]^C
BbeI          117  GGCGCC               GGCGC^C
BlpI          432  GCTGAGC              GC^T[ACGT]AGC
BpmI          322  CTGGAG               CTGGAG(16/14)
Bpu1102I      432  GCTGAGC              GC^T[ACGT]AGC
BsaWI         179  ACCGGT               [AT]^CCGG[AT]
BsiEI         155  CGGCCG               CG[GA][CT]^CG
BsrBI          58  GAGCGG               CCGCTC(-3/-3)
BssSI         298  CTCGTG               CACGAG(-5/-1)
BstEII        339  GGTGACC              G^GT[ACGT]ACC
BstPI         339  GGTGACC              G^GT[ACGT]ACC
CelII         432  GCTGAGC              GC^T[ACGT]AGC
DraII         511  AGGACCC              [GA]G^G[ACGT]CC[CT]
EagI          155  CGGCCG               C^GGCCG
EclXI         155  CGGCCG               C^GGCCG
Eco52I        155  CGGCCG               C^GGCCG
EcoO109I      511  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       339  GGTGACC              G^GT[ACGT]ACC
EcoT14I        29  CCATGG               C^C[AT][AT]GG
HpaI          573  GTTAAC               GTT^AAC
KasI          117  GGCGCC               G^GCGCC
MaeII         398  ACGT                 A^CGT
MseI          574  TTAA                 T^TAA
MslI           24  CACCACCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          114  GCCGGC               GCC^GGC
NarI          117  GGCGCC               GG^CGCC
NcoI           29  CCATGG               C^CATGG
NgoMIV        114  GCCGGC               G^CCGGC
NotI          154  GCGGCCGC             GC^GGCCGC
PflMI         414  CCAGCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         179  ACCGGT               A^CCGGT
PpuMI         511  AGGACCC              [GA]G^G[AT]CC[CT]
PstI          469  CTGCAG               CTGCA^G
PvuII         544  CAGCTG               CAG^CTG
RsaI           84  GTAC                 GT^AC
SacI          435  GAGCTC               GAGCT^C
SfcI          469  CTGCAG               C^T[GA][CT]AG
SfoI          117  GGCGCC               GGC^GCC
StyI           29  CCATGG               C^C[AT][AT]GG
TaqI          224  TCGA                 T^CGA
Tru9I         574  TTAA                 T^TAA
TspRI         524  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       224  TCGA                 T^CGA
Van91I        414  CCAGCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          523  CCACTGGCTTTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          216  GAACCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcyI          117  GGCGCC               G[GA]^CG[CT]C
              397  GACGTC              
AspHI         253  GTGCTC               G[AT]GC[AT]^C
              435  GAGCTC              
BanI          103  GGCACC               G^G[CT][GA]CC
              117  GGCGCC              
BmyI          253  GTGCTC               G[AGT]GC[ACT]^C
              435  GAGCTC              
BsaHI         117  GGCGCC               G[GA]^CG[CT]C
              397  GACGTC              
BsiHKAI       253  GTGCTC               G[AT]GC[AT]^C
              435  GAGCTC              
BsmFI         313  GGGAC                GGGAC(10/14)
              407  GGGAC               
Bsp1286I      253  GTGCTC               G[AGT]GC[ACT]^C
              435  GAGCTC              
BsrFI         114  GCCGGC               [GA]^CCGG[CT]
              179  ACCGGT              
BsrI          525  ACTGG                ACTGG(1/-1)
                9  CCAGT               
BstF5I        122  CATCC                GGATG(2/0)
              489  GGATG               
Cfr10I        114  GCCGGC               [GA]^CCGG[CT]
              179  ACCGGT              
DdeI          433  CTGAG                C^T[ACGT]AG
              476  CTCAG               
FokI          122  CATCC                GGATG(9/13)
              489  GGATG               
HgaI          294  GACGC                GACGC(5/10)
              315  GACGC               
Hin1I         117  GGCGCC               G[GA]^CG[CT]C
              397  GACGTC              
HincII          4  GTTGAC               GT[CT]^[GA]AC
              573  GTTAAC              
HindII          4  GTTGAC               GT[CT]^[GA]AC
              573  GTTAAC              
HinfI         174  GATTC                G^A[ACGT]TC
              247  GATTC               
KspI          152  CCGCGG               CCGC^GG
              373  CCGCGG              
MboII         354  GAAGA                GAAGA(8/7)
              506  TCTTC               
SacII         152  CCGCGG               CCGC^GG
              373  CCGCGG              
TfiI          174  GATTC                G^A[AT]TC
              247  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AccII       AciI        AluI        AvaI        AvaII       
BbvI        BcnI        BsaJI       BsiYI       BslI        
BsoBI       BssKI       BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DsaI        EaeI        EcoRII      
FauI        Fnu4HI      HaeII       HaeIII      HapII       
HhaI        HinP1I      HpaII       HphI        ItaI        
MaeIII      MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NlaIII      NlaIV       
Sau96I      ScrFI       SmaI        TseI        Tsp45I      
XmaCI       XmaI        
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