Restriction map for pF1KB6434
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# NO CUT ENZYMES (  127 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
AspEI       AviII       BalI        BbeI        BbrPI       
BbsI        BclI        BfrI        BglII       BpuAI       
BsaAI       BsaHI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspLU11I    BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoT22I     FbaI        FseI        FspI        Hin1I       
HincII      HindII      HpaI        KasI        KspI        
MaeIII      MfeI        MluI        MluNI       MroI        
MscI        MunI        NarI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Tsp45I      XbaI        XhoI        
XmaCI       XmaI        


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# ONE CUT ENZYMES (   60 enzymes) #
###################################

Acc65I        195  GGTACC               G^GTACC
AfaI          196  GTAC                 GT^AC
Asp718I       195  GGTACC               G^GTACC
AspI          760  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          751  CCCGAG               C^[CT]CG[GA]G
AvrII         951  CCTAGG               C^CTAGG
BanII         807  GGGCTC               G[GA]GC[CT]^C
BcgI          728  GCAGACACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         596  GGATAC               GTATCC(6/5)
BcnI          762  CCCGG                CC^[GC]GG
BglI          582  GCCAGATCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          951  CCTAGG               C^CTAGG
BlpI          670  GCTCAGC              GC^T[ACGT]AGC
BpmI          897  CTCCAG               CTGGAG(16/14)
Bpu1102I      670  GCTCAGC              GC^T[ACGT]AGC
BsaBI         255  GATCCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          680  GAGACC               GGTCTC(1/5)
BsaWI         348  ACCGGA               [AT]^CCGG[AT]
BsoBI         751  CCCGAG               C^[CT]CG[GA]G
BspHI         534  TCATGA               T^CATGA
BspMI         632  GCAGGT               ACCTGC(4/8)
BsrDI        1005  CATTGC               GCAATG(2/0)
BsrFI         325  GCCGGC               [GA]^CCGG[CT]
BstAPI        661  GCAGAAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII         670  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        325  GCCGGC               [GA]^CCGG[CT]
DraII          47  AGGTCCT              [GA]G^G[ACGT]CC[CT]
DsaI          983  CCATGG               C^C[GA][CT]GG
EaeI            2  TGGCCG               [CT]^GGCC[GA]
EarI          819  GAAGAG               CTCTTC(1/4)
EcoNI         258  CCTGATCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       47  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoRV         538  GATATC               GAT^ATC
HgaI          244  GACGC                GACGC(5/10)
HindIII       207  AAGCTT               A^AGCTT
KpnI          195  GGTACC               GGTAC^C
Ksp632I       819  GAAGAG               CTCTTC(1/4)
MaeII         548  ACGT                 A^CGT
MamI          255  GATCCTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI          307  TTAA                 T^TAA
MslI          906  CACCCTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        199  CCGCTG               C[AC]G^C[GT]G
NaeI          325  GCCGGC               GCC^GGC
NciI          762  CCCGG                CC^[GC]GG
NcoI          983  CCATGG               C^CATGG
NdeI          442  CATATG               CA^TATG
NgoMIV        325  GCCGGC               G^CCGGC
PflFI         760  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         441  CCATATGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI          47  AGGTCCT              [GA]G^G[AT]CC[CT]
PstI          390  CTGCAG               CTGCA^G
RcaI          534  TCATGA               T^CATGA
RsaI          196  GTAC                 GT^AC
SapI          819  GAAGAGC              GCTCTTC(1/4)
TaqI          263  TCGA                 T^CGA
Tru9I         307  TTAA                 T^TAA
Tsp509I       657  AATT                 ^AATT
Tth111I       760  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       263  TCGA                 T^CGA
Van91I        441  CCATATGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccII           6  CGCG                 CG^CG
              246  CGCG                
Asp700I       841  GAAGGTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              888  GAACTTCTTC          
BamHI         186  GGATCC               G^GATCC
              254  GGATCC              
BbvCI         270  GCTGAGG              CCTCAGC(-5/-2)
              918  GCTGAGG             
BsmAI         680  GAGAC                GTCTC(1/5)
               58  GTCTC               
BsrBI         932  CCGCTC               CCGCTC(-3/-3)
              225  GAGCGG              
BsrI          110  ACTGG                ACTGG(1/-1)
              470  ACTGG               
BstUI           6  CGCG                 CG^CG
              246  CGCG                
CfoI          856  GCGC                 GCG^C
              995  GCGC                
EcoT14I       951  CCTAGG               C^C[AT][AT]GG
              983  CCATGG              
FauI          692  CCCGC                CCCGC(4/6)
              247  GCGGG               
HaeII         855  GGCGCT               [GA]GCGC^[CT]
              994  AGCGCC              
HhaI          856  GCGC                 GCG^C
              995  GCGC                
HinP1I        856  GCGC                 G^CGC
              995  GCGC                
HinfI         505  GACTC                G^A[ACGT]TC
              788  GACTC               
MvnI            6  CGCG                 CG^CG
              246  CGCG                
PleI          505  GACTC                GAGTC(4/5)
              788  GACTC               
SfaNI         319  GATGC                GCATC(5/9)
              720  GATGC               
SfcI          390  CTGCAG               C^T[GA][CT]AG
              990  CTATAG              
StyI          951  CCTAGG               C^C[AT][AT]GG
              983  CCATGG              
XcmI          774  CCAGGGCAAGATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              794  CCATCCTGTGCGTGG     
XmnI          841  GAAGGTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              888  GAACTTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AciI        AluI        AlwI        AspHI       AvaII       
BanI        BbvI        BfaI        BmyI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmFI       
Bsp1286I    BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HpaII       
HphI        ItaI        MaeI        MboI        MboII       
MflI        MnlI        MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
ScrFI       TseI        TspRI       XhoII       
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