Restriction map for pF1KB6434
 (Linear) (Six-base) MAPSORT of: KIBB6434.seq  Check: 7905  from: 1  to: 1032



KIBB6434.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

Acc65I G'GTAC_C

Cuts at:      0     195    1032 
   Size:        195     837
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     687     856    1032 
   Size:        687     169     176

  Fragments arranged by size: 

                 687     176     169
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     880     912    1032 
   Size:        880      32     120

  Fragments arranged by size: 

                 880     120      32
 
AvaI C'yCGr_G

Cuts at:      0     751    1032 
   Size:        751     281
 
AvrII C'CTAG_G

Cuts at:      0     951    1032 
   Size:        951      81
 
BamHI G'GATC_C

Cuts at:      0     186     254    1032 
   Size:        186      68     778

  Fragments arranged by size: 

                 778     186      68
 
BanI G'GyrC_C

Cuts at:      0     195     579     971    1032 
   Size:        195     384     392      61

  Fragments arranged by size: 

                 392     384     195      61
 
BanII G_rGCy'C

Cuts at:      0     811    1032 
   Size:        811     221
 
BbvCI CC'TCA_GC

Cuts at:      0     271     919    1032 
   Size:        271     648     113

  Fragments arranged by size: 

                 648     271     113
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     717     751    1032 
   Size:        717      34     281

  Fragments arranged by size: 

                 717     281      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     590    1032 
   Size:        590     442
 
BglI GCCn_nnn'nGGC

Cuts at:      0     588    1032 
   Size:        588     444
 
BlpI GC'TnA_GC

Cuts at:      0     671    1032 
   Size:        671     361
 
Bme1580I G_kGCm'C

Cuts at:      0     974    1032 
   Size:        974      58
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     382    1032 
   Size:        382     650
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     882    1032 
   Size:        882     150
 
Bpu10I CC'TnA_GC

Cuts at:      0     271     919    1032 
   Size:        271     648     113

  Fragments arranged by size: 

                 648     271     113
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     674    1032 
   Size:        674     358
 
BsaBI GATnn'nnATC

Cuts at:      0     259    1032 
   Size:        259     773
 
BsaWI w'CCGG_w

Cuts at:      0     348    1032 
   Size:        348     684
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     880     910    1032 
   Size:        880      30     122

  Fragments arranged by size: 

                 880     122      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     280     521     918     967    1032 
   Size:        280     241     397      49      65

  Fragments arranged by size: 

                 397     280     241      65      49
 
BseYI C'CCAG_C

Cuts at:      0     434     640     712     723     824    1032 
   Size:        434     206      72      11     101     208

  Fragments arranged by size: 

                 434     208     206     101      72      11
 
BsiHKAI G_wGCw'C

Cuts at:      0     103     368     672    1032 
   Size:        103     265     304     360

  Fragments arranged by size: 

                 360     304     265     103
 
Bsp1286I G_dGCh'C

Cuts at:      0     103     368     672     811     974    1032 
   Size:        103     265     304     139     163      58

  Fragments arranged by size: 

                 304     265     163     139     103      58
 
BspHI T'CATG_A

Cuts at:      0     534    1032 
   Size:        534     498
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     623    1032 
   Size:        623     409
 
BsrBI CCG'CTC

Cuts at:      0     227     934    1032 
   Size:        227     707      98

  Fragments arranged by size: 

                 707     227      98
 
BsrDI GCAATG_nn'

Cuts at:      0    1004    1032 
   Size:       1004      28
 
BsrFI r'CCGG_y

Cuts at:      0     325    1032 
   Size:        325     707
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     667    1032 
   Size:        667     365
 
BstYI r'GATC_y

Cuts at:      0      74     186     254     875    1032 
   Size:         74     112      68     621     157

  Fragments arranged by size: 

                 621     157     112      74      68
 
BtgI C'CryG_G

Cuts at:      0     983    1032 
   Size:        983      49
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     318    1032 
   Size:        318     714
 
BtsI GCAGTG_nn'

Cuts at:      0     494     749    1032 
   Size:        494     255     283

  Fragments arranged by size: 

                 494     283     255
 
EaeI y'GGCC_r

Cuts at:      0       2    1032 
   Size:          2    1030
 
EarI CTCTTCn'nnn_

Cuts at:      0     814    1032 
   Size:        814     218
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     687     856     882    1032 
   Size:        687     169      26     150

  Fragments arranged by size: 

                 687     169     150      26
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     262    1032 
   Size:        262     770
 
EcoO109I rG'GnC_Cy

Cuts at:      0      48    1032 
   Size:         48     984
 
EcoRV GAT'ATC

Cuts at:      0     540    1032 
   Size:        540     492
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     865    1032 
   Size:        865     167
 
HaeII r_GCGC'y

Cuts at:      0     859     998    1032 
   Size:        859     139      34

  Fragments arranged by size: 

                 859     139      34
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      32      64     588     620     779     811     867     899 
   Size:         32      32     524      32     159      32      56      32

Cuts at:    899    1032 
   Size:        133

  Fragments arranged by size: 

                 524     159     133      56      32      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0     207    1032 
   Size:        207     825
 
KpnI G_GTAC'C

Cuts at:      0     199    1032 
   Size:        199     833
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     410    1032 
   Size:        410     622
 
MslI CAynn'nnrTG

Cuts at:      0     910    1032 
   Size:        910     122
 
MspA1I CmG'CkG

Cuts at:      0     201    1032 
   Size:        201     831
 
NaeI GCC'GGC

Cuts at:      0     327    1032 
   Size:        327     705
 
NcoI C'CATG_G

Cuts at:      0     983    1032 
   Size:        983      49
 
NdeI CA'TA_TG

Cuts at:      0     443    1032 
   Size:        443     589
 
NgoMIV G'CCGG_C

Cuts at:      0     325    1032 
   Size:        325     707
 
PasI CC'CwG_GG

Cuts at:      0     864    1032 
   Size:        864     168
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     447    1032 
   Size:        447     585
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     880     912    1032 
   Size:        880      32     120

  Fragments arranged by size: 

                 880     120      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      48    1032 
   Size:         48     984
 
PstI C_TGCA'G

Cuts at:      0     394    1032 
   Size:        394     638
 
SapI GCTCTTCn'nnn_

Cuts at:      0     814    1032 
   Size:        814     218
 
SfcI C'TryA_G

Cuts at:      0     390     990    1032 
   Size:        390     600      42

  Fragments arranged by size: 

                 600     390      42
 
StyI C'CwwG_G

Cuts at:      0     951     983    1032 
   Size:        951      32      49

  Fragments arranged by size: 

                 951      49      32
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     293     439     928    1032 
   Size:        293     146     489     104

  Fragments arranged by size: 

                 489     293     146     104
 
Tth111I GACn'n_nGTC

Cuts at:      0     763    1032 
   Size:        763     269
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     781     801    1032 
   Size:        781      20     231

  Fragments arranged by size: 

                 781     231      20
 
XmnI GAAnn'nnTTC

Cuts at:      0     845     892    1032 
   Size:        845      47     140

  Fragments arranged by size: 

                 845     140      47
 

 Enzymes that do cut:

   Acc65I     AcuI     AloI     AvaI    AvrII    BamHI     BanI    BanII
    BbvCI     BcgI    BciVI     BglI     BlpI Bme1580I     BplI     BpmI
   Bpu10I     BsaI    BsaBI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI
 Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BstAPI    BstYI
     BtgI    BtgZI     BtsI     EaeI     EarI  Eco57MI    EcoNI EcoO109I
    EcoRV     FalI    HaeII    Hin4I  HindIII     KpnI     MmeI     MslI
   MspA1I     NaeI     NcoI     NdeI   NgoMIV     PasI    PflMI     PpiI
    PpuMI     PstI     SapI     SfcI     StyI    TaqII  Tth111I     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     BaeI     BbeI     BbsI     BclI    BfrBI    BglII
    BmgBI     BmrI     BmtI    BpuEI    BsaAI    BsaHI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoRI     FseI     FspI    FspAI
   HincII     HpaI     KasI     MfeI     MluI     MscI     NarI     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI     PfoI     PmeI
     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI     XbaI
     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com