Restriction map for pF1KE0037
 (Linear) (Six-base) MAPSORT of: KIEE0037.seq  Check: 7264  from: 1  to: 2220



KIEE0037.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AccI GT'mk_AC

Cuts at:      0    1010    2220 
   Size:       1010    1210
 
Acc65I G'GTAC_C

Cuts at:      0     710    2220 
   Size:        710    1510
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1210    1592    1668    2150    2220 
   Size:       1210     382      76     482      70

  Fragments arranged by size: 

                1210     482     382      76      70
 
AfeI AGC'GCT

Cuts at:      0     256    2220 
   Size:        256    1964
 
AhdI GACnn_n'nnGTC

Cuts at:      0     270    2220 
   Size:        270    1950
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     173     951    1813    2220 
   Size:        173     778     862     407

  Fragments arranged by size: 

                 862     778     407     173
 
AlwNI CAG_nnn'CTG

Cuts at:      0     117     246     655    1432    1894    2122    2220 
   Size:        117     129     409     777     462     228      98

  Fragments arranged by size: 

                 777     462     409     228     129     117      98
 
ApaI G_GGCC'C

Cuts at:      0     364    1827    2220 
   Size:        364    1463     393

  Fragments arranged by size: 

                1463     393     364
 
ApaLI G'TGCA_C

Cuts at:      0    1117    2220 
   Size:       1117    1103
 
ApoI r'AATT_y

Cuts at:      0    1273    2220 
   Size:       1273     947
 
AvaI C'yCGr_G

Cuts at:      0     221    1677    2079    2220 
   Size:        221    1456     402     141

  Fragments arranged by size: 

                1456     402     221     141
 
BamHI G'GATC_C

Cuts at:      0     167    1460    2220 
   Size:        167    1293     760

  Fragments arranged by size: 

                1293     760     167
 
BanI G'GyrC_C

Cuts at:      0     556     710    1144    1198    2049    2220 
   Size:        556     154     434      54     851     171

  Fragments arranged by size: 

                 851     556     434     171     154      54
 
BanII G_rGCy'C

Cuts at:      0     364     682    1166    1259    1827    2065    2220 
   Size:        364     318     484      93     568     238     155

  Fragments arranged by size: 

                 568     484     364     318     238     155      93
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     521    1389    2220 
   Size:        521     868     831

  Fragments arranged by size: 

                 868     831     521
 
BbvCI CC'TCA_GC

Cuts at:      0    1027    1244    2141    2220 
   Size:       1027     217     897      79

  Fragments arranged by size: 

                1027     897     217      79
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1203    1237    2220 
   Size:       1203      34     983

  Fragments arranged by size: 

                1203     983      34
 
BglII A'GATC_T

Cuts at:      0    1815    2220 
   Size:       1815     405
 
BlpI GC'TnA_GC

Cuts at:      0     676    2158    2220 
   Size:        676    1482      62

  Fragments arranged by size: 

                1482     676      62
 
Bme1580I G_kGCm'C

Cuts at:      0     364    1121    1201    1827    2052    2220 
   Size:        364     757      80     626     225     168

  Fragments arranged by size: 

                 757     626     364     225     168      80
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     377     940    2220 
   Size:        377     563    1280

  Fragments arranged by size: 

                1280     563     377
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     756     898    1437    2220 
   Size:        756     142     539     783

  Fragments arranged by size: 

                 783     756     539     142
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     154     177     198    1042    1180    1852    2085 
   Size:        145       9      23      21     844     138     672     233

Cuts at:   2085    2200    2220 
   Size:        115      20

  Fragments arranged by size: 

                 844     672     233     145     138     115      23      21
                  20       9
 
Bpu10I CC'TnA_GC

Cuts at:      0    1027    1244    1441    2141    2220 
   Size:       1027     217     197     700      79

  Fragments arranged by size: 

                1027     700     217     197      79
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     130     869    2220 
   Size:        130     739    1351

  Fragments arranged by size: 

                1351     739     130
 
BsaAI yAC'GTr

Cuts at:      0     302    1086    2220 
   Size:        302     784    1134

  Fragments arranged by size: 

                1134     784     302
 
BsaHI Gr'CG_yC

Cuts at:      0    1206    1375    2220 
   Size:       1206     169     845

  Fragments arranged by size: 

                1206     845     169
 
BsaWI w'CCGG_w

Cuts at:      0     392     826    1463    1595    1955    2220 
   Size:        392     434     637     132     360     265

  Fragments arranged by size: 

                 637     434     392     360     265     132
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     196     226     467     497    2220 
   Size:        196      30     241      30    1723

  Fragments arranged by size: 

                1723     241     196      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     415     475    1261    1557    2220 
   Size:        415      60     786     296     663

  Fragments arranged by size: 

                 786     663     415     296      60
 
BseYI C'CCAG_C

Cuts at:      0      24      30      54     631     909     981    1112    1135 
   Size:         24       6      24     577     278      72     131      23

Cuts at:   1135    1235    1504    1808    2220 
   Size:        100     269     304     412

  Fragments arranged by size: 

                 577     412     304     278     269     131     100      72
                  24      24      23       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259    1387    2220 
   Size:        259    1128     833

  Fragments arranged by size: 

                1128     833     259
 
BsiEI CG_ry'CG

Cuts at:      0     290    2220 
   Size:        290    1930
 
BsiHKAI G_wGCw'C

Cuts at:      0     682     930    1121    1166    2045    2220 
   Size:        682     248     191      45     879     175

  Fragments arranged by size: 

                 879     682     248     191     175      45
 
BsiWI C'GTAC_G

Cuts at:      0     302    2220 
   Size:        302    1918
 
Bsp1286I G_dGCh'C

Cuts at:      0     364     682     930    1121    1166    1201    1259    1827 
   Size:        364     318     248     191      45      35      58     568

Cuts at:   1827    2045    2052    2065    2220 
   Size:        218       7      13     155

  Fragments arranged by size: 

                 568     364     318     248     218     191     155      58
                  45      35      13       7
 
BspEI T'CCGG_A

Cuts at:      0     826    1955    2220 
   Size:        826    1129     265

  Fragments arranged by size: 

                1129     826     265
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     722     887    1941    2220 
   Size:        722     165    1054     279

  Fragments arranged by size: 

                1054     722     279     165
 
BsrBI CCG'CTC

Cuts at:      0    1421    2220 
   Size:       1421     799
 
BsrDI GCAATG_nn'

Cuts at:      0     560    1577    2220 
   Size:        560    1017     643

  Fragments arranged by size: 

                1017     643     560
 
BsrGI T'GTAC_A

Cuts at:      0     644    2220 
   Size:        644    1576
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     246    1894    2220 
   Size:        246    1648     326

  Fragments arranged by size: 

                1648     326     246
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     102     348     917    1047    2220 
   Size:        102     246     569     130    1173

  Fragments arranged by size: 

                1173     569     246     130     102
 
BstYI r'GATC_y

Cuts at:      0     128     167    1415    1460    1616    1815    2125    2220 
   Size:        128      39    1248      45     156     199     310      95

  Fragments arranged by size: 

                1248     310     199     156     128      95      45      39
            
 
Bsu36I CC'TnA_GG

Cuts at:      0     723    1360    2200    2220 
   Size:        723     637     840      20

  Fragments arranged by size: 

                 840     723     637      20
 
BtgI C'CryG_G

Cuts at:      0    1059    1268    1571    2220 
   Size:       1059     209     303     649

  Fragments arranged by size: 

                1059     649     303     209
 
BtsI GCAGTG_nn'

Cuts at:      0      10    1146    1877    1921    2220 
   Size:         10    1136     731      44     299

  Fragments arranged by size: 

                1136     731     299      44      10
 
EaeI y'GGCC_r

Cuts at:      0      69     287    2220 
   Size:         69     218    1933

  Fragments arranged by size: 

                1933     218      69
 
EagI C'GGCC_G

Cuts at:      0     287    2220 
   Size:        287    1933
 
EarI CTCTTCn'nnn_

Cuts at:      0     801    1041    1277    2220 
   Size:        801     240     236     943

  Fragments arranged by size: 

                 943     801     240     236
 
EcoICRI GAG'CTC

Cuts at:      0     680    1164    2220 
   Size:        680     484    1056

  Fragments arranged by size: 

                1056     680     484
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     154     177     198    1042    1180    1210    1592 
   Size:        145       9      23      21     844     138      30     382

Cuts at:   1592    1668    1852    2085    2150    2200    2220 
   Size:         76     184     233      65      50      20

  Fragments arranged by size: 

                 844     382     233     184     145     138      76      65
                  50      30      23      21      20       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2100    2220 
   Size:       2100     120
 
EcoO109I rG'GnC_Cy

Cuts at:      0     114     231     264     361     480     762     952    1181 
   Size:        114     117      33      97     119     282     190     229

Cuts at:   1181    1429    1823    1824    1855    2220 
   Size:        248     394       1      31     365

  Fragments arranged by size: 

                 394     365     282     248     229     190     119     117
                 114      97      33      31       1
 
EcoRI G'AATT_C

Cuts at:      0    1273    2220 
   Size:       1273     947
 
HaeII r_GCGC'y

Cuts at:      0     258    2220 
   Size:        258    1962
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     256     288    2220 
   Size:        256      32    1932

  Fragments arranged by size: 

                1932     256      32
 
HindIII A'AGCT_T

Cuts at:      0    2132    2220 
   Size:       2132      88
 
KpnI G_GTAC'C

Cuts at:      0     714    2220 
   Size:        714    1506
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1064    2220 
   Size:       1064    1156
 
MslI CAynn'nnrTG

Cuts at:      0    1327    1339    2167    2220 
   Size:       1327      12     828      53

  Fragments arranged by size: 

                1327     828      53      12
 
MspA1I CmG'CkG

Cuts at:      0     153     243    1112    1714    2220 
   Size:        153      90     869     602     506

  Fragments arranged by size: 

                 869     602     506     153      90
 
NcoI C'CATG_G

Cuts at:      0    1571    2220 
   Size:       1571     649
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     117     707    1432    2220 
   Size:        117     590     725     788

  Fragments arranged by size: 

                 788     725     590     117
 
PfoI T'CCnGG_A

Cuts at:      0     131     170     863    1157    2220 
   Size:        131      39     693     294    1063

  Fragments arranged by size: 

                1063     693     294     131      39
 
PpuMI rG'GwC_Cy

Cuts at:      0     114     231     264     762     952    1181    2220 
   Size:        114     117      33     498     190     229    1039

  Fragments arranged by size: 

                1039     498     229     190     117     114      33
 
PspOMI G'GGCC_C

Cuts at:      0     360    1823    2220 
   Size:        360    1463     397

  Fragments arranged by size: 

                1463     397     360
 
PstI C_TGCA'G

Cuts at:      0     254    1304    1802    1872    1899    1928    1952    2220 
   Size:        254    1050     498      70      27      29      24     268

  Fragments arranged by size: 

                1050     498     268     254      70      29      27      24
            
 
PvuII CAG'CTG

Cuts at:      0     153     243    1112    2220 
   Size:        153      90     869    1108

  Fragments arranged by size: 

                1108     869     153      90
 
SacI G_AGCT'C

Cuts at:      0     682    1166    2220 
   Size:        682     484    1054

  Fragments arranged by size: 

                1054     682     484
 
SanDI GG'GwC_CC

Cuts at:      0     762     952    1181    2220 
   Size:        762     190     229    1039

  Fragments arranged by size: 

                1039     762     229     190
 
SbfI CC_TGCA'GG

Cuts at:      0    1802    1952    2220 
   Size:       1802     150     268

  Fragments arranged by size: 

                1802     268     150
 
SexAI A'CCwGG_T

Cuts at:      0     233    2220 
   Size:        233    1987
 
SfcI C'TryA_G

Cuts at:      0     250     529     942    1300    1773    1798    1868    1895 
   Size:        250     279     413     358     473      25      70      27

Cuts at:   1895    1924    1948    2031    2220 
   Size:         29      24      83     189

  Fragments arranged by size: 

                 473     413     358     279     250     189      83      70
                  29      27      25      24
 
SmaI CCC'GGG

Cuts at:      0    1679    2081    2220 
   Size:       1679     402     139

  Fragments arranged by size: 

                1679     402     139
 
StuI AGG'CCT

Cuts at:      0     278     295    2205    2220 
   Size:        278      17    1910      15

  Fragments arranged by size: 

                1910     278      17      15
 
StyI C'CwwG_G

Cuts at:      0     599    1571    1901    2220 
   Size:        599     972     330     319

  Fragments arranged by size: 

                 972     599     330     319
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1104    1510    2220 
   Size:       1104     406     710

  Fragments arranged by size: 

                1104     710     406
 
TatI w'GTAC_w

Cuts at:      0     644    2220 
   Size:        644    1576
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     196     228     467     499    1191    1223    2220 
   Size:        196      32     239      32     692      32     997

  Fragments arranged by size: 

                 997     692     239     196      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1816    2220 
   Size:       1816     404
 
XmaI C'CCGG_G

Cuts at:      0    1677    2079    2220 
   Size:       1677     402     141

  Fragments arranged by size: 

                1677     402     141
 
XmnI GAAnn'nnTTC

Cuts at:      0     695    2220 
   Size:        695    1525
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI     AhdI     AlfI    AlwNI     ApaI
    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI
     BcgI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BsrGI   BstAPI    BstXI    BstYI   Bsu36I     BtgI     BtsI     EaeI
     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII
    Hin4I  HindIII     KpnI     MmeI     MslI   MspA1I     NcoI    PflMI
     PfoI    PpuMI   PspOMI     PstI    PvuII     SacI    SanDI     SbfI
    SexAI     SfcI     SmaI     StuI     StyI    TaqII     TatI     TstI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI    AflII   AflIII     AgeI     AleI     AloI
     AscI     AseI    AsiSI    AvrII     BaeI     BbeI    BciVI     BclI
    BfrBI     BglI    BmgBI     BmtI    BpuEI    BsaBI     BsmI    BsmBI
    BspHI    BsrFI   BssHII    BssSI    BstBI   BstEII  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EciI    EcoRV     FalI
     FseI     FspI    FspAI   HincII     HpaI     KasI     MfeI     MluI
     MscI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
     SapI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XhoI
     ZraI
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