Restriction map for pF1KB7954
 (Linear) (Six-base) MAPSORT of: KIBB7954.seq  Check: 8123  from: 1  to: 1185



KIBB7954.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     578     704     713     968    1033    1147    1185 
   Size:        578     126       9     255      65     114      38

  Fragments arranged by size: 

                 578     255     126     114      65      38       9
 
AflII C'TTAA_G

Cuts at:      0     271    1185 
   Size:        271     914
 
AflIII A'CryG_T

Cuts at:      0     302    1185 
   Size:        302     883
 
AlwNI CAG_nnn'CTG

Cuts at:      0     238     766    1185 
   Size:        238     528     419

  Fragments arranged by size: 

                 528     419     238
 
ApoI r'AATT_y

Cuts at:      0     403     600     836     849    1185 
   Size:        403     197     236      13     336

  Fragments arranged by size: 

                 403     336     236     197      13
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     614     647    1185 
   Size:        614      33     538

  Fragments arranged by size: 

                 614     538      33
 
BanI G'GyrC_C

Cuts at:      0     775    1185 
   Size:        775     410
 
BanII G_rGCy'C

Cuts at:      0      90    1185 
   Size:         90    1095
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1153    1185 
   Size:       1153      32
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1061    1185 
   Size:       1061     124
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     581     713    1185 
   Size:        581     132     472

  Fragments arranged by size: 

                 581     472     132
 
Bpu10I CC'TnA_GC

Cuts at:      0     687    1185 
   Size:        687     498
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     554     584    1085    1115    1185 
   Size:        554      30     501      30      70

  Fragments arranged by size: 

                 554     501      70      30      30
 
BseYI C'CCAG_C

Cuts at:      0     108    1185 
   Size:        108    1077
 
BsiHKAI G_wGCw'C

Cuts at:      0      90    1185 
   Size:         90    1095
 
Bsp1286I G_dGCh'C

Cuts at:      0      90    1185 
   Size:         90    1095
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      37    1185 
   Size:         37    1148
 
BsrDI GCAATG_nn'

Cuts at:      0     941    1185 
   Size:        941     244
 
BsrGI T'GTAC_A

Cuts at:      0     387    1185 
   Size:        387     798
 
BssSI C'ACGA_G

Cuts at:      0      41    1185 
   Size:         41    1144
 
EaeI y'GGCC_r

Cuts at:      0     737    1185 
   Size:        737     448
 
EarI CTCTTCn'nnn_

Cuts at:      0     660    1028    1052    1185 
   Size:        660     368      24     133

  Fragments arranged by size: 

                 660     368     133      24
 
EcoICRI GAG'CTC

Cuts at:      0      88    1185 
   Size:         88    1097
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     578     581     704     713     968    1033    1147    1185 
   Size:        578       3     123       9     255      65     114      38

  Fragments arranged by size: 

                 578     255     123     114      65      38       9       3
            
 
EcoRI G'AATT_C

Cuts at:      0     836    1185 
   Size:        836     349
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     303     335     977    1009    1085    1117    1185 
   Size:        303      32     642      32      76      32      68

  Fragments arranged by size: 

                 642     303      76      68      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0      25    1185 
   Size:         25    1160
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     889    1124    1185 
   Size:        889     235      61

  Fragments arranged by size: 

                 889     235      61
 
MscI TGG'CCA

Cuts at:      0     739    1185 
   Size:        739     446
 
MslI CAynn'nnrTG

Cuts at:      0     892    1185 
   Size:        892     293
 
MspA1I CmG'CkG

Cuts at:      0    1059    1185 
   Size:       1059     126
 
NdeI CA'TA_TG

Cuts at:      0     227    1185 
   Size:        227     958
 
NspI r_CATG'y

Cuts at:      0     306     532    1185 
   Size:        306     226     653

  Fragments arranged by size: 

                 653     306     226
 
PciI A'CATG_T

Cuts at:      0     302    1185 
   Size:        302     883
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     871    1185 
   Size:        871     314
 
PsiI TTA'TAA

Cuts at:      0     961    1185 
   Size:        961     224
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     623     655    1185 
   Size:        623      32     530

  Fragments arranged by size: 

                 623     530      32
 
PstI C_TGCA'G

Cuts at:      0     762    1026    1185 
   Size:        762     264     159

  Fragments arranged by size: 

                 762     264     159
 
PvuII CAG'CTG

Cuts at:      0    1059    1185 
   Size:       1059     126
 
SacI G_AGCT'C

Cuts at:      0      90    1185 
   Size:         90    1095
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1052    1185 
   Size:       1052     133
 
SfcI C'TryA_G

Cuts at:      0      98     758    1022    1131    1185 
   Size:         98     660     264     109      54

  Fragments arranged by size: 

                 660     264     109      98      54
 
SmlI C'TyrA_G

Cuts at:      0     271    1185 
   Size:        271     914
 
StuI AGG'CCT

Cuts at:      0    1129    1185 
   Size:       1129      56
 
StyI C'CwwG_G

Cuts at:      0     608    1185 
   Size:        608     577
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     227    1185 
   Size:        227     958
 
TatI w'GTAC_w

Cuts at:      0     387    1185 
   Size:        387     798
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     554     586    1185 
   Size:        554      32     599

  Fragments arranged by size: 

                 599     554      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     204    1185 
   Size:        204     981
 
XmnI GAAnn'nnTTC

Cuts at:      0     654    1046    1185 
   Size:        654     392     139

  Fragments arranged by size: 

                 654     392     139
 

 Enzymes that do cut:

     AcuI    AflII   AflIII    AlwNI     ApoI     BaeI     BanI    BanII
     BbsI     BplI     BpmI   Bpu10I    BsaXI    BseYI  BsiHKAI Bsp1286I
    BspMI    BsrDI    BsrGI    BssSI     EaeI     EarI  EcoICRI  Eco57MI
    EcoRI    Hin4I  HindIII     MmeI     MscI     MslI   MspA1I     NdeI
     NspI     PciI    PflMI     PsiI     PsrI     PstI    PvuII     SacI
     SapI     SfcI     SmlI     StuI     StyI    TaqII     TatI     TstI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI
   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PvuI    RsrII    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XhoI
     XmaI     ZraI
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