(Circular) (Six-base) MAPSORT of: pF1KB7926.seq Check: 3494 from: 1 to: 4163 pF1KB7926 4163 bp With 182 enzymes: SgfI * March 2, 2010 14:27 .. AccI GT'mk_AC Cuts at: 1201 1201 Size: 4163 Acc65I G'GTAC_C Cuts at: 1179 1179 Size: 4163 AclI AA'CG_TT Cuts at: 4003 4003 Size: 4163 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 309 1168 1988 2420 3167 3435 309 Size: 859 820 432 747 268 1037 Fragments arranged by size: 1037 859 820 747 432 268 AflIII A'CryG_T Cuts at: 966 1515 2619 3596 966 Size: 549 1104 977 1533 Fragments arranged by size: 1533 1104 977 549 AgeI A'CCGG_T Cuts at: 2551 2551 Size: 4163 AlwNI CAG_nnn'CTG Cuts at: 1468 3035 1468 Size: 1567 2596 ApaLI G'TGCA_C Cuts at: 2933 2933 Size: 4163 ApoI r'AATT_y Cuts at: 976 1167 3516 3772 976 Size: 191 2349 256 1367 Fragments arranged by size: 2349 1367 256 191 AseI AT'TA_AT Cuts at: 20 476 20 Size: 456 3707 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4163 AvaI C'yCGr_G Cuts at: 1183 1183 Size: 4163 BamHI G'GATC_C Cuts at: 1188 1188 Size: 4163 BanI G'GyrC_C Cuts at: 243 1179 1593 1841 1876 243 Size: 936 414 248 35 2530 Fragments arranged by size: 2530 936 414 248 35 BanII G_rGCy'C Cuts at: 267 1177 2207 267 Size: 910 1030 2223 Fragments arranged by size: 2223 1030 910 BbeI G_GCGC'C Cuts at: 247 1597 1845 247 Size: 1350 248 2565 Fragments arranged by size: 2565 1350 248 BbsI GAAGACnn'nnnn_ Cuts at: 550 550 Size: 4163 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 148 182 148 Size: 34 4129 BciVI GTATCCnnnnn_n' Cuts at: 2061 2822 4163 2061 Size: 761 1341 2061 Fragments arranged by size: 2061 1341 761 BclI T'GATC_A Cuts at: 704 1035 1683 704 Size: 331 648 3184 Fragments arranged by size: 3184 648 331 BfrBI ATG'CAT Cuts at: 738 738 Size: 4163 BglII A'GATC_T Cuts at: 814 1678 3715 814 Size: 864 2037 1262 Fragments arranged by size: 2037 1262 864 BlpI GC'TnA_GC Cuts at: 1275 1275 Size: 4163 Bme1580I G_kGCm'C Cuts at: 1788 1881 2937 1788 Size: 93 1056 3014 Fragments arranged by size: 3014 1056 93 BmgBI CAC'GTC Cuts at: 1078 1078 Size: 4163 BmrI ACTGGGnnnn_n' Cuts at: 1375 1456 1791 3935 1375 Size: 81 335 2144 1603 Fragments arranged by size: 2144 1603 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2530 2530 Size: 4163 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 319 397 451 898 319 Size: 78 54 447 3584 Fragments arranged by size: 3584 447 78 54 Bpu10I CC'TnA_GC Cuts at: 293 293 Size: 4163 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1339 2218 2710 3008 1339 Size: 879 492 298 2494 Fragments arranged by size: 2494 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3867 3867 Size: 4163 BsaAI yAC'GTr Cuts at: 1516 1516 Size: 4163 BsaBI GATnn'nnATC Cuts at: 6 1677 3714 6 Size: 1671 2037 455 Fragments arranged by size: 2037 1671 455 BsaHI Gr'CG_yC Cuts at: 244 1594 1842 2544 4040 244 Size: 1350 248 702 1496 367 Fragments arranged by size: 1496 1350 702 367 248 BsaWI w'CCGG_w Cuts at: 1352 1873 2551 2825 2972 3383 1352 Size: 521 678 274 147 411 2132 Fragments arranged by size: 2132 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 249 279 249 Size: 30 4133 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 247 367 385 394 406 409 412 415 475 Size: 120 18 9 12 3 3 3 60 Cuts at: 475 478 1133 247 Size: 3 655 3277 Fragments arranged by size: 3277 655 120 60 18 12 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 1588 2346 2923 3419 1588 Size: 758 577 496 2332 Fragments arranged by size: 2332 758 577 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 200 200 Size: 4163 BsiEI CG_ry'CG Cuts at: 104 1705 1751 2959 104 Size: 1601 46 1208 1308 Fragments arranged by size: 1601 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1177 1955 2145 2937 1177 Size: 778 190 792 2403 Fragments arranged by size: 2403 792 778 190 BsmBI CGTCTCn'nnnn_ Cuts at: 250 250 Size: 4163 Bsp1286I G_dGCh'C Cuts at: 267 1177 1788 1881 1955 2145 2207 2937 267 Size: 910 611 93 74 190 62 730 1493 Fragments arranged by size: 1493 910 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3383 3383 Size: 4163 BspHI T'CATG_A Cuts at: 3339 3339 Size: 4163 BspMI ACCTGCnnnn'nnnn_ Cuts at: 786 1213 1427 1729 2110 786 Size: 427 214 302 381 2839 Fragments arranged by size: 2839 427 381 302 214 BsrBI CCG'CTC Cuts at: 50 2455 2509 3993 4158 50 Size: 2405 54 1484 165 55 Fragments arranged by size: 2405 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1103 2075 1103 Size: 972 3191 BsrFI r'CCGG_y Cuts at: 240 440 2161 2342 2551 240 Size: 200 1721 181 209 1852 Fragments arranged by size: 1852 1721 209 200 181 BsrGI T'GTAC_A Cuts at: 692 692 Size: 4163 BssSI C'ACGA_G Cuts at: 2434 2792 2434 Size: 358 3805 BstAPI GCAn_nnn'nTGC Cuts at: 1540 1540 Size: 4163 BstBI TT'CG_AA Cuts at: 2524 2524 Size: 4163 BstYI r'GATC_y Cuts at: 1 814 1188 1655 1678 2013 2259 3271 3357 Size: 813 374 467 23 335 246 1012 86 Cuts at: 3357 3369 3715 1 Size: 12 346 449 Fragments arranged by size: 1012 813 467 449 374 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 4163 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2113 2294 2457 2113 Size: 181 163 3819 Fragments arranged by size: 3819 181 163 BtsI GCAGTG_nn' Cuts at: 1406 1406 Size: 4163 DraI TTT'AAA Cuts at: 1162 1162 Size: 4163 DrdI GACnn_nn'nnGTC Cuts at: 1869 2727 1869 Size: 858 3305 EaeI y'GGCC_r Cuts at: 1748 1922 2313 2340 3685 1748 Size: 174 391 27 1345 2226 Fragments arranged by size: 2226 1345 391 174 27 EagI C'GGCC_G Cuts at: 1748 1748 Size: 4163 EarI CTCTTCn'nnn_ Cuts at: 362 383 2186 2396 362 Size: 21 1803 210 2129 Fragments arranged by size: 2129 1803 210 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 173 2371 2681 2827 3973 173 Size: 2198 310 146 1146 363 Fragments arranged by size: 2198 1146 363 310 146 EcoICRI GAG'CTC Cuts at: 1175 1175 Size: 4163 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 309 319 397 451 898 1168 1988 2420 3167 Size: 10 78 54 447 270 820 432 747 Cuts at: 3167 3435 309 Size: 268 1037 Fragments arranged by size: 1037 820 747 447 432 270 268 78 54 10 EcoO109I rG'GnC_Cy Cuts at: 1302 1302 Size: 4163 EcoRI G'AATT_C Cuts at: 1167 1167 Size: 4163 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2182 2182 Size: 4163 FspI TGC'GCA Cuts at: 1944 1944 Size: 4163 HaeII r_GCGC'y Cuts at: 149 247 471 1597 1845 2867 3846 149 Size: 98 224 1126 248 1022 979 466 Fragments arranged by size: 1126 1022 979 466 248 224 98 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 249 281 633 665 2005 2035 2037 2067 249 Size: 32 352 32 1340 30 2 30 2345 Fragments arranged by size: 2345 1340 352 32 32 30 30 2 HincII GTy'rAC Cuts at: 1202 1202 Size: 4163 KasI G'GCGC_C Cuts at: 243 1593 1841 243 Size: 1350 248 2565 Fragments arranged by size: 2565 1350 248 KpnI G_GTAC'C Cuts at: 1183 1183 Size: 4163 MluI A'CGCG_T Cuts at: 3596 3596 Size: 4163 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 338 647 1025 1098 2834 3018 338 Size: 309 378 73 1736 184 1483 Fragments arranged by size: 1736 1483 378 309 184 73 MscI TGG'CCA Cuts at: 1924 1924 Size: 4163 MslI CAynn'nnrTG Cuts at: 2152 2279 2152 Size: 127 4036 MspA1I CmG'CkG Cuts at: 226 1274 1588 1948 2961 3206 226 Size: 1048 314 360 1013 245 1183 Fragments arranged by size: 1183 1048 1013 360 314 245 NaeI GCC'GGC Cuts at: 442 2344 442 Size: 1902 2261 NarI GG'CG_CC Cuts at: 244 1594 1842 244 Size: 1350 248 2565 Fragments arranged by size: 2565 1350 248 NcoI C'CATG_G Cuts at: 107 107 Size: 4163 NgoMIV G'CCGG_C Cuts at: 440 2342 440 Size: 1902 2261 NsiI A_TGCA'T Cuts at: 740 740 Size: 4163 NspI r_CATG'y Cuts at: 970 1216 1716 2623 970 Size: 246 500 907 2510 Fragments arranged by size: 2510 907 500 246 PciI A'CATG_T Cuts at: 966 2619 966 Size: 1653 2510 PmeI GTTT'AAAC Cuts at: 1162 1162 Size: 4163 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3302 3334 3302 Size: 32 4131 PshAI GACnn'nnGTC Cuts at: 410 410 Size: 4163 PsiI TTA'TAA Cuts at: 656 656 Size: 4163 PstI C_TGCA'G Cuts at: 171 1210 1895 171 Size: 1039 685 2439 Fragments arranged by size: 2439 1039 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4163 PvuII CAG'CTG Cuts at: 1588 1948 1588 Size: 360 3803 RsrII CG'GwC_CG Cuts at: 2358 2358 Size: 4163 SacI G_AGCT'C Cuts at: 1177 1177 Size: 4163 SalI G'TCGA_C Cuts at: 1200 1200 Size: 4163 SapI GCTCTTCn'nnn_ Cuts at: 2186 2396 2186 Size: 210 3953 SbfI CC_TGCA'GG Cuts at: 1210 1210 Size: 4163 ScaI AGT'ACT Cuts at: 3680 3680 Size: 4163 SfcI C'TryA_G Cuts at: 33 167 600 1206 1891 2884 3075 33 Size: 134 433 606 685 993 191 1121 Fragments arranged by size: 1121 993 685 606 433 191 134 SfoI GGC'GCC Cuts at: 245 1595 1843 245 Size: 1350 248 2565 Fragments arranged by size: 2565 1350 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4163 SmaI CCC'GGG Cuts at: 1185 1185 Size: 4163 SmlI C'TyrA_G Cuts at: 1318 2233 2725 2987 1318 Size: 915 492 262 2494 Fragments arranged by size: 2494 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4163 SphI G_CATG'C Cuts at: 1216 1716 1216 Size: 500 3663 StyI C'CwwG_G Cuts at: 107 1297 1651 107 Size: 1190 354 2619 Fragments arranged by size: 2619 1190 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 686 1746 1879 2338 2547 686 Size: 1060 133 459 209 2302 Fragments arranged by size: 2302 1060 459 209 133 TatI w'GTAC_w Cuts at: 633 692 3678 633 Size: 59 2986 1118 Fragments arranged by size: 2986 1118 59 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 779 811 3680 3712 779 Size: 32 2869 32 1230 Fragments arranged by size: 2869 1230 32 32 Tth111I GACn'n_nGTC Cuts at: 1960 1960 Size: 4163 XbaI T'CTAG_A Cuts at: 711 762 1194 711 Size: 51 432 3680 Fragments arranged by size: 3680 432 51 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1658 1658 Size: 4163 XmaI C'CCGG_G Cuts at: 1183 1183 Size: 4163 XmnI GAAnn'nnTTC Cuts at: 557 557 Size: 4163 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BglI BmtI BsiWI BsmI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI PacI PasI PflMI PfoI PmlI PpuMI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI