Restriction map for pF1KE0040
##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       Alw44I      
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspHI       AviII       AvrII       
BamHI       BbeI        BbsI        BbvCI       BclI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaHI       BseAI       BsiHKAI     BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstUI       BstZ17I     CelII       
ClaI        CpoI        DrdI        Eco47III    EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HincII      HindII      
HpaI        KasI        KpnI        KspI        MfeI        
MluI        MroI        MunI        MvnI        NarI        
NcoI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AfaI          255  GTAC                 GT^AC
AflIII       1817  ACATGT               A^C[GA][CT]GT
AgeI         1545  ACCGGT               A^CCGGT
AhdI         1875  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1415  CCTCAGG              CC^T[ACGT]AGG
Asp700I       579  GAAAGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1875  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          805  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        1666  CACGTG               CAC^GTG
BcgI         1835  GCAGGGACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1006  GTATCC               GTATCC(6/5)
BglI         1606  GCCCAGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        1666  CACGTG               [CT]AC^GT[GA]
BsaBI        1140  GATCCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          511  GGTCTC               GGTCTC(1/5)
BsaWI        1545  ACCGGT               [AT]^CCGG[AT]
BspLU11I     1817  ACATGT               A^CATGT
BsrI         1876  ACTGG                ACTGG(1/-1)
BstEII       1635  GGTGACC              G^GT[ACGT]ACC
BstPI        1635  GGTGACC              G^GT[ACGT]ACC
Bsu36I       1415  CCTCAGG              CC^T[ACGT]AGG
CfoI          729  GCGC                 GCG^C
DraI          874  TTTAAA               TTT^AAA
DraIII       1663  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1529  CCGTGG               C^C[GA][CT]GG
Eam1105I     1875  GACTGGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1415  CCTCAGG              CC^T[ACGT]AGG
EcoNI         669  CCTTAACGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1635  GGTGACC              G^GT[ACGT]ACC
HhaI          729  GCGC                 GCG^C
HinP1I        729  GCGC                 G^CGC
HindIII      1558  AAGCTT               A^AGCTT
MamI         1140  GATCCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI          197  GCCGGC               GCC^GGC
NgoMIV        197  GCCGGC               G^CCGGC
PflFI         805  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1545  ACCGGT               A^CCGGT
PmaCI        1666  CACGTG               CAC^GTG
PmlI         1666  CACGTG               CAC^GTG
PstI         1790  CTGCAG               CTGCA^G
RsaI          255  GTAC                 GT^AC
ScaI          254  AGTACT               AGT^ACT
SfcI         1790  CTGCAG               C^T[GA][CT]AG
Tsp45I       1636  GTGAC                ^GT[GC]AC
Tsp509I      1171  AATT                 ^AATT
Tth111I       805  GACACTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI          579  GAAAGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

ApaI           90  GGGCCC               GGGCC^C
             1752  GGGCCC              
AvaI          265  CCCGAG               C^[CT]CG[GA]G
             1748  CTCGGG              
BalI          823  TGGCCA               TGG^CCA
             1259  TGGCCA              
BanI          546  GGTGCC               G^G[CT][GA]CC
              840  GGTGCC              
BsgI         1355  GTGCAG               GTGCAG(16/14)
             1690  GTGCAG              
BsiEI         195  CGGCCG               CG[GA][CT]^CG
              394  CGGCCG              
BsmAI        1338  GAGAC                GTCTC(1/5)
              512  GTCTC               
BsoBI         265  CCCGAG               C^[CT]CG[GA]G
             1748  CTCGGG              
BsrBI        1210  CCGCTC               CCGCTC(-3/-3)
             1507  GAGCGG              
BstAPI        141  GCAGTTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1044  GCAGGAGCTGC         
EagI          195  CGGCCG               C^GGCCG
              394  CGGCCG              
EarI           69  CTCTTC               CTCTTC(1/4)
                6  GAAGAG              
EclXI         195  CGGCCG               C^GGCCG
              394  CGGCCG              
Eco52I        195  CGGCCG               C^GGCCG
              394  CGGCCG              
Ksp632I        69  CTCTTC               CTCTTC(1/4)
                6  GAAGAG              
MaeII         401  ACGT                 A^CGT
             1667  ACGT                
MaeIII        457  GTTAC                ^GT[ACGT]AC
             1636  GTGAC               
MluNI         823  TGGCCA               TGG^CCA
             1259  TGGCCA              
MscI          823  TGGCCA               TGG^CCA
             1259  TGGCCA              
MseI          671  TTAA                 T^TAA
              875  TTAA                
SmlI          976  CTCAAG               C^T[CT][GA]AG
             1579  CTCAAG              
StuI          666  AGGCCT               AGG^CCT
              869  AGGCCT              
Tru9I         671  TTAA                 T^TAA
              875  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   75 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AvaII       
BanII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaJI       BseRI       BsiYI       BslI        
BsmFI       Bsp1286I    BspMI       BsrFI       BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
EaeI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        MaeI        MboI        
MboII       MflI        MnlI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PvuII       Sau3AI      Sau96I      ScrFI       
SfaNI       SphI        StyI        TaqI        TfiI        
TseI        TspRI       TthHB8I     Van91I      XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com