Restriction map for pF1KE0040
 (Linear) (Six-base) MAPSORT of: KIEE0040.seq  Check: 6835  from: 1  to: 1908



KIEE0040.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1031    1908 
   Size:       1031     877
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     484    1508    1908 
   Size:        484    1024     400

  Fragments arranged by size: 

                1024     484     400
 
AflIII A'CryG_T

Cuts at:      0    1817    1908 
   Size:       1817      91
 
AgeI A'CCGG_T

Cuts at:      0    1545    1908 
   Size:       1545     363
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1880    1908 
   Size:       1880      28
 
AleI CACnn'nnGTG

Cuts at:      0     544    1762    1908 
   Size:        544    1218     146

  Fragments arranged by size: 

                1218     544     146
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     967    1908 
   Size:        967     941
 
AlwNI CAG_nnn'CTG

Cuts at:      0     147     432    1050    1317    1908 
   Size:        147     285     618     267     591

  Fragments arranged by size: 

                 618     591     285     267     147
 
ApaI G_GGCC'C

Cuts at:      0      94    1756    1908 
   Size:         94    1662     152

  Fragments arranged by size: 

                1662     152      94
 
AvaI C'yCGr_G

Cuts at:      0     265    1748    1908 
   Size:        265    1483     160

  Fragments arranged by size: 

                1483     265     160
 
BanI G'GyrC_C

Cuts at:      0     546     840    1908 
   Size:        546     294    1068

  Fragments arranged by size: 

                1068     546     294
 
BanII G_rGCy'C

Cuts at:      0      94    1749    1756    1908 
   Size:         94    1655       7     152

  Fragments arranged by size: 

                1655     152      94       7
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1824    1858    1908 
   Size:       1824      34      50

  Fragments arranged by size: 

                1824      50      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1017    1908 
   Size:       1017     891
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1612    1908 
   Size:       1612     296
 
Bme1580I G_kGCm'C

Cuts at:      0      94    1756    1908 
   Size:         94    1662     152

  Fragments arranged by size: 

                1662     152      94
 
BmgBI CAC'GTC

Cuts at:      0     402    1908 
   Size:        402    1506
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     409     853    1233    1637    1717    1908 
   Size:        409     444     380     404      80     191

  Fragments arranged by size: 

                 444     409     404     380     191      80
 
Bpu10I CC'TnA_GC

Cuts at:      0    1384    1675    1908 
   Size:       1384     291     233

  Fragments arranged by size: 

                1384     291     233
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     961    1564    1908 
   Size:        961     603     344

  Fragments arranged by size: 

                 961     603     344
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     517    1908 
   Size:        517    1391
 
BsaAI yAC'GTr

Cuts at:      0    1668    1908 
   Size:       1668     240
 
BsaBI GATnn'nnATC

Cuts at:      0    1144    1908 
   Size:       1144     764
 
BsaWI w'CCGG_w

Cuts at:      0    1545    1908 
   Size:       1545     363
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     283     328     862    1289    1867    1908 
   Size:        283      45     534     427     578      41

  Fragments arranged by size: 

                 578     534     427     283      45      41
 
BseYI C'CCAG_C

Cuts at:      0      74     419     494     624    1030    1307    1317    1424 
   Size:         74     345      75     130     406     277      10     107

Cuts at:   1424    1607    1908 
   Size:        183     301

  Fragments arranged by size: 

                 406     345     301     277     183     130     107      75
                  74      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1376    1711    1908 
   Size:       1376     335     197

  Fragments arranged by size: 

                1376     335     197
 
BsiEI CG_ry'CG

Cuts at:      0     198     397    1908 
   Size:        198     199    1511

  Fragments arranged by size: 

                1511     199     198
 
Bsp1286I G_dGCh'C

Cuts at:      0      94    1749    1756    1908 
   Size:         94    1655       7     152

  Fragments arranged by size: 

                1655     152      94       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     221     308     949    1031    1908 
   Size:        221      87     641      82     877

  Fragments arranged by size: 

                 877     641     221      87      82
 
BsrBI CCG'CTC

Cuts at:      0    1212    1509    1908 
   Size:       1212     297     399

  Fragments arranged by size: 

                1212     399     297
 
BsrFI r'CCGG_y

Cuts at:      0     197    1130    1545    1908 
   Size:        197     933     415     363

  Fragments arranged by size: 

                 933     415     363     197
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     147    1050    1908 
   Size:        147     903     858

  Fragments arranged by size: 

                 903     858     147
 
BstEII G'GTnAC_C

Cuts at:      0    1635    1908 
   Size:       1635     273
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     624    1257    1397    1615    1764    1908 
   Size:        624     633     140     218     149     144

  Fragments arranged by size: 

                 633     624     218     149     144     140
 
BstYI r'GATC_y

Cuts at:      0     218     317     362    1727    1908 
   Size:        218      99      45    1365     181

  Fragments arranged by size: 

                1365     218     181      99      45
 
Bsu36I CC'TnA_GG

Cuts at:      0    1416    1908 
   Size:       1416     492
 
BtgI C'CryG_G

Cuts at:      0    1529    1908 
   Size:       1529     379
 
BtsI GCAGTG_nn'

Cuts at:      0     826    1799    1908 
   Size:        826     973     109

  Fragments arranged by size: 

                 973     826     109
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1860    1895    1908 
   Size:       1860      35      13

  Fragments arranged by size: 

                1860      35      13
 
DraI TTT'AAA

Cuts at:      0     876    1908 
   Size:        876    1032
 
DraIII CAC_nnn'GTG

Cuts at:      0    1668    1908 
   Size:       1668     240
 
EaeI y'GGCC_r

Cuts at:      0     195     394     823    1259    1796    1908 
   Size:        195     199     429     436     537     112

  Fragments arranged by size: 

                 537     436     429     199     195     112
 
EagI C'GGCC_G

Cuts at:      0     195     394    1908 
   Size:        195     199    1514

  Fragments arranged by size: 

                1514     199     195
 
EarI CTCTTCn'nnn_

Cuts at:      0       1      75    1908 
   Size:          1      74    1833

  Fragments arranged by size: 

                1833      74       1
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     150     162    1908 
   Size:        150      12    1746

  Fragments arranged by size: 

                1746     150      12
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     409     484     853    1233    1508    1637    1717    1908 
   Size:        409      75     369     380     275     129      80     191

  Fragments arranged by size: 

                 409     380     369     275     191     129      80      75
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     673    1908 
   Size:        673    1235
 
EcoO109I rG'GnC_Cy

Cuts at:      0      90     276     711     938    1034    1206    1284    1420 
   Size:         90     186     435     227      96     172      78     136

Cuts at:   1420    1752    1753    1776    1908 
   Size:        332       1      23     132

  Fragments arranged by size: 

                 435     332     227     186     172     136     132      96
                  90      78      23       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     786    1908 
   Size:        786    1122
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     838     870    1268    1284    1300    1316    1357    1389 
   Size:        838      32     398      16      16      16      41      32

Cuts at:   1389    1866    1898    1908 
   Size:        477      32      10

  Fragments arranged by size: 

                 838     477     398      41      32      32      32      16
                  16      16      10
 
HindIII A'AGCT_T

Cuts at:      0    1558    1908 
   Size:       1558     350
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1829    1908 
   Size:       1829      79
 
MscI TGG'CCA

Cuts at:      0     825    1261    1908 
   Size:        825     436     647

  Fragments arranged by size: 

                 825     647     436
 
MslI CAynn'nnrTG

Cuts at:      0     544     916     932    1687    1762    1908 
   Size:        544     372      16     755      75     146

  Fragments arranged by size: 

                 755     544     372     146      75      16
 
MspA1I CmG'CkG

Cuts at:      0     282     429     964    1314    1611    1647    1695    1887 
   Size:        282     147     535     350     297      36      48     192

Cuts at:   1887    1908 
   Size:         21

  Fragments arranged by size: 

                 535     350     297     282     192     147      48      36
                  21
 
NaeI GCC'GGC

Cuts at:      0     199    1908 
   Size:        199    1709
 
NgoMIV G'CCGG_C

Cuts at:      0     197    1908 
   Size:        197    1711
 
NspI r_CATG'y

Cuts at:      0     160     264    1099    1107    1821    1908 
   Size:        160     104     835       8     714      87

  Fragments arranged by size: 

                 835     714     160     104      87       8
 
PciI A'CATG_T

Cuts at:      0    1817    1908 
   Size:       1817      91
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     432    1268    1668    1908 
   Size:        432     836     400     240

  Fragments arranged by size: 

                 836     432     400     240
 
PfoI T'CCnGG_A

Cuts at:      0     686    1908 
   Size:        686    1222
 
PmlI CAC'GTG

Cuts at:      0    1668    1908 
   Size:       1668     240
 
PpuMI rG'GwC_Cy

Cuts at:      0     276     938    1206    1284    1420    1908 
   Size:        276     662     268      78     136     488

  Fragments arranged by size: 

                 662     488     276     268     136      78
 
PspOMI G'GGCC_C

Cuts at:      0      90    1752    1908 
   Size:         90    1662     156

  Fragments arranged by size: 

                1662     156      90
 
PstI C_TGCA'G

Cuts at:      0    1794    1908 
   Size:       1794     114
 
PvuII CAG'CTG

Cuts at:      0     964    1611    1647    1695    1908 
   Size:        964     647      36      48     213

  Fragments arranged by size: 

                 964     647     213      48      36
 
SanDI GG'GwC_CC

Cuts at:      0     276    1206    1284    1908 
   Size:        276     930      78     624

  Fragments arranged by size: 

                 930     624     276      78
 
ScaI AGT'ACT

Cuts at:      0     256    1908 
   Size:        256    1652
 
SfcI C'TryA_G

Cuts at:      0    1790    1908 
   Size:       1790     118
 
SmlI C'TyrA_G

Cuts at:      0     976    1579    1908 
   Size:        976     603     329

  Fragments arranged by size: 

                 976     603     329
 
SphI G_CATG'C

Cuts at:      0     160     264    1099    1908 
   Size:        160     104     835     809

  Fragments arranged by size: 

                 835     809     160     104
 
StuI AGG'CCT

Cuts at:      0     668     871    1908 
   Size:        668     203    1037

  Fragments arranged by size: 

                1037     668     203
 
StyI C'CwwG_G

Cuts at:      0     866     891    1631    1908 
   Size:        866      25     740     277

  Fragments arranged by size: 

                 866     740     277      25
 
TatI w'GTAC_w

Cuts at:      0     254    1908 
   Size:        254    1654
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1333    1365    1908 
   Size:       1333      32     543

  Fragments arranged by size: 

                1333     543      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     808    1908 
   Size:        808    1100
 
XmnI GAAnn'nnTTC

Cuts at:      0     583    1908 
   Size:        583    1325
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI
     ApaI     AvaI     BanI    BanII     BcgI    BciVI     BglI Bme1580I
    BmgBI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI
    BseRI    BseYI     BsgI    BsiEI Bsp1286I    BspMI    BsrBI    BsrFI
   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI    CspCI
     DraI   DraIII     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I     FalI    Hin4I  HindIII     MmeI     MscI     MslI   MspA1I
     NaeI   NgoMIV     NspI     PciI    PflMI     PfoI     PmlI    PpuMI
   PspOMI     PstI    PvuII    SanDI     ScaI     SfcI     SmlI     SphI
     StuI     StyI     TatI     TstI  Tth111I     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AloI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BbsI    BbvCI     BclI    BfrBI    BglII     BlpI     BmrI     BmtI
     BplI    BsaHI    BsaXI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrDI    BsrGI   BssHII    BssSI    BstBI  BstZ17I    BtgZI
     ClaI     DrdI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI
    TaqII     XbaI     XcmI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com