(Linear) (Six-base) MAPSORT of: KIEE0017.seq  Check: 5833  from: 1  to: 1650



KIEE0017.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AclI AA'CG_TT

Cuts at:      0     984    1650 
   Size:        984     666
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     135     527    1094    1650 
   Size:        135     392     567     556

  Fragments arranged by size: 

                 567     556     392     135
 
AflIII A'CryG_T

Cuts at:      0    1406    1650 
   Size:       1406     244
 
AhdI GACnn_n'nnGTC

Cuts at:      0     336    1650 
   Size:        336    1314
 
AleI CACnn'nnGTG

Cuts at:      0    1234    1650 
   Size:       1234     416
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1236    1650 
   Size:       1236     414
 
AlwNI CAG_nnn'CTG

Cuts at:      0     943    1465    1650 
   Size:        943     522     185

  Fragments arranged by size: 

                 943     522     185
 
ApoI r'AATT_y

Cuts at:      0     249    1650 
   Size:        249    1401
 
AvrII C'CTAG_G

Cuts at:      0     421    1650 
   Size:        421    1229
 
BanII G_rGCy'C

Cuts at:      0      31    1650 
   Size:         31    1619
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      38     173     356     614    1650 
   Size:         38     135     183     258    1036

  Fragments arranged by size: 

                1036     258     183     135      38
 
BclI T'GATC_A

Cuts at:      0    1146    1650 
   Size:       1146     504
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1549    1650 
   Size:       1549     101
 
Bme1580I G_kGCm'C

Cuts at:      0    1614    1650 
   Size:       1614      36
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      25    1276    1650 
   Size:         25    1251     374

  Fragments arranged by size: 

                1251     374      25
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1064    1650 
   Size:       1064     586
 
Bpu10I CC'TnA_GC

Cuts at:      0    1133    1650 
   Size:       1133     517
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     748     809    1650 
   Size:        748      61     841

  Fragments arranged by size: 

                 841     748      61
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1422    1585    1650 
   Size:       1422     163      65

  Fragments arranged by size: 

                1422     163      65
 
BsaBI GATnn'nnATC

Cuts at:      0     902    1650 
   Size:        902     748
 
BsaHI Gr'CG_yC

Cuts at:      0     382    1650 
   Size:        382    1268
 
BseYI C'CCAG_C

Cuts at:      0    1333    1650 
   Size:       1333     317
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1431    1650 
   Size:       1431     219
 
BsmI GAATG_Cn'

Cuts at:      0     211     862     955    1650 
   Size:        211     651      93     695

  Fragments arranged by size: 

                 695     651     211      93
 
Bsp1286I G_dGCh'C

Cuts at:      0      31    1614    1650 
   Size:         31    1583      36

  Fragments arranged by size: 

                1583      36      31
 
BspHI T'CATG_A

Cuts at:      0      57    1650 
   Size:         57    1593
 
BsrDI GCAATG_nn'

Cuts at:      0     816    1344    1454    1650 
   Size:        816     528     110     196

  Fragments arranged by size: 

                 816     528     196     110
 
BsrFI r'CCGG_y

Cuts at:      0    1127    1650 
   Size:       1127     523
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1042    1650 
   Size:       1042     608
 
BtgI C'CryG_G

Cuts at:      0    1281    1650 
   Size:       1281     369
 
BtsI GCAGTG_nn'

Cuts at:      0      71     696    1175    1419    1650 
   Size:         71     625     479     244     231

  Fragments arranged by size: 

                 625     479     244     231      71
 
DraIII CAC_nnn'GTG

Cuts at:      0    1282    1650 
   Size:       1282     368
 
EarI CTCTTCn'nnn_

Cuts at:      0     116    1310    1412    1650 
   Size:        116    1194     102     238

  Fragments arranged by size: 

                1194     238     116     102
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    1650 
   Size:         19    1631
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     135     527    1064    1094    1650 
   Size:        135     392     537      30     556

  Fragments arranged by size: 

                 556     537     392     135      30
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     322     354     448     480    1650 
   Size:        322      32      94      32    1170

  Fragments arranged by size: 

                1170     322      94      32      32
 
HindIII A'AGCT_T

Cuts at:      0     864    1085    1650 
   Size:        864     221     565

  Fragments arranged by size: 

                 864     565     221
 
MslI CAynn'nnrTG

Cuts at:      0      95    1234    1650 
   Size:         95    1139     416

  Fragments arranged by size: 

                1139     416      95
 
NspI r_CATG'y

Cuts at:      0     449     877    1410    1650 
   Size:        449     428     533     240

  Fragments arranged by size: 

                 533     449     428     240
 
PciI A'CATG_T

Cuts at:      0    1406    1650 
   Size:       1406     244
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     177     209     863     895    1650 
   Size:        177      32     654      32     755

  Fragments arranged by size: 

                 755     654     177      32      32
 
PstI C_TGCA'G

Cuts at:      0     720    1650 
   Size:        720     930
 
SfcI C'TryA_G

Cuts at:      0     716    1650 
   Size:        716     934
 
SmlI C'TyrA_G

Cuts at:      0     763     824    1650 
   Size:        763      61     826

  Fragments arranged by size: 

                 826     763      61
 
StuI AGG'CCT

Cuts at:      0     426     820    1650 
   Size:        426     394     830

  Fragments arranged by size: 

                 830     426     394
 
StyI C'CwwG_G

Cuts at:      0     421    1650 
   Size:        421    1229
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1081    1492    1650 
   Size:       1081     411     158

  Fragments arranged by size: 

                1081     411     158
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     675     707    1650 
   Size:        675      32     943

  Fragments arranged by size: 

                 943     675      32
 
XbaI T'CTAG_A

Cuts at:      0     195    1650 
   Size:        195    1455
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1545    1650 
   Size:       1545     105
 

 Enzymes that do cut:

     AclI     AcuI   AflIII     AhdI     AleI     AlfI    AlwNI     ApoI
    AvrII    BanII     BbsI     BclI     BglI Bme1580I     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BseYI     BsgI     BsmI
 Bsp1286I    BspHI    BsrDI    BsrFI    BstXI     BtgI     BtsI   DraIII
     EarI     EciI  Eco57MI    Hin4I  HindIII     MslI     NspI     PciI
     PpiI     PstI     SfcI     SmlI     StuI     StyI    TaqII     TstI
     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI     BaeI    BamHI
     BanI     BbeI    BbvCI     BcgI    BciVI    BfrBI    BglII     BlpI
    BmgBI     BmtI     BplI    BsaAI    BsaWI    BsaXI    BseRI    BsiEI
  BsiHKAI    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI     TatI  Tth111I     XhoI     XmaI
     XmnI     ZraI