Restriction map for pF1KA0175
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflIII      AgeI        AocI        
Aor51HI     AscI        Asp700I     Asp718I     AspI        
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaHI       
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BspDI       BspEI       BspHI       BspLU11I    BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EaeI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HpaI        
KasI        KpnI        KspI        MaeII       MfeI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        NaeI        NarI        NcoI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SwaI        Tth111I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AflII        1891  CTTAAG               C^TTAAG
AhdI         1045  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        367  GTGCAC               G^TGCAC
ApaI         1657  GGGCCC               GGGCC^C
ApaLI         367  GTGCAC               G^TGCAC
AseI         1110  ATTAAT               AT^TAAT
AsnI         1110  ATTAAT               AT^TAAT
AspEI        1045  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI         1505  GGTGCC               G^G[CT][GA]CC
BanII        1657  GGGCCC               G[GA]GC[CT]^C
BclI          930  TGATCA               T^GATCA
BfrI         1891  CTTAAG               C^TTAAG
BglI          964  GCCAAGAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmBI        1652  GAGACG               CGTCTC(1/5)
BsmI          707  GCATTC               GAATGC(1/-1)
BsrBI        1509  CCGCTC               CCGCTC(-3/-3)
BstYI        1524  GGATCT               [GA]^GATC[CT]
DraII         392  GGGACCT              [GA]G^G[ACGT]CC[CT]
Eam1105I     1045  GACATCAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I      392  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       470  CCAAGG               C^C[AT][AT]GG
FauI         1105  GCGGG                CCCGC(4/6)
FbaI          930  TGATCA               T^GATCA
HindIII      1672  AAGCTT               A^AGCTT
MflI         1524  GGATCT               [GA]^GATC[CT]
MspA1I       1885  CAGCGG               C[AC]G^C[GT]G
NdeI         1540  CATATG               CA^TATG
PflMI        1181  CCAAGTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         392  GGGACCT              [GA]G^G[AT]CC[CT]
PshBI        1110  ATTAAT               AT^TAAT
PstI          730  CTGCAG               CTGCA^G
SfaNI        1900  GATGC                GCATC(5/9)
SmaI          326  CCCGGG               CCC^GGG
StuI          176  AGGCCT               AGG^CCT
StyI          470  CCAAGG               C^C[AT][AT]GG
TaqI          845  TCGA                 T^CGA
TthHB8I       845  TCGA                 T^CGA
Van91I       1181  CCAAGTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI           87  CCATATCCTTACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1524  GGATCT               [GA]^GATC[CT]
XmaCI         326  CCCGGG               C^CCGGG
XmaI          326  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AcsI          408  AAATTT               [GA]^AATT[CT]
             1452  AAATTC              
ApoI          408  AAATTT               [GA]^AATT[CT]
             1452  AAATTC              
AspHI         367  GTGCAC               G[AT]GC[AT]^C
             1618  GTGCTC              
AvaI          326  CCCGGG               C^[CT]CG[GA]G
              974  CTCGGG              
AvaII         393  GGACC                G^G[AT]CC
              738  GGACC               
BsaBI         421  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              484  GATTACCATC          
BsaI          322  GAGACC               GGTCTC(1/5)
              454  GGTCTC              
BsiHKAI       367  GTGCAC               G[AT]GC[AT]^C
             1618  GTGCTC              
BsmFI          52  GGGAC                GGGAC(10/14)
              392  GGGAC               
BsoBI         326  CCCGGG               C^[CT]CG[GA]G
              974  CTCGGG              
Bsp1407I      873  TGTACA               T^GTACA
             1767  TGTACA              
BspMI        1245  ACCTGC               ACCTGC(4/8)
              732  GCAGGT              
BsrGI         873  TGTACA               T^GTACA
             1767  TGTACA              
BstF5I        778  CATCC                GGATG(2/0)
             1932  CATCC               
Cac8I          82  GCCTGC               GC[ACGT]^[ACGT]GC
             1847  GCCAGC              
EarI          316  GAAGAG               CTCTTC(1/4)
             1306  GAAGAG              
FokI          778  CATCC                GGATG(9/13)
             1932  CATCC               
HaeIII        177  GGCC                 GG^CC
             1658  GGCC                
HapII         156  CCGG                 C^CGG
              327  CCGG                
HincII        209  GTCAAC               GT[CT]^[GA]AC
             1759  GTTGAC              
HindII        209  GTCAAC               GT[CT]^[GA]AC
             1759  GTTGAC              
HpaII         156  CCGG                 C^CGG
              327  CCGG                
Ksp632I       316  GAAGAG               CTCTTC(1/4)
             1306  GAAGAG              
MamI          421  GATGAATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              484  GATTACCATC          
MspI          156  CCGG                 C^CGG
              327  CCGG                
MwoI          517  GCTTATGCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              964  GCCAAGAAGGC         
NspI          499  ACATGC               [GA]CATG^[CT]
             1755  GCATGT              
PshAI         448  GACTTTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1479  GACAGGTGTC          
SfcI          493  CTACAG               C^T[GA][CT]AG
              730  CTGCAG              
SspBI         873  TGTACA               T^GTACA
             1767  TGTACA              
SspI           26  AATATT               AAT^ATT
              243  AATATT              


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BseRI       BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstNI       Cfr13I      DdeI        DpnII       EcoRII      
Fnu4HI      HinfI       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MvaI        NciI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScaI        ScrFI       SmlI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       
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