(Circular) (Six-base) MAPSORT of: pF1KA0175.seq Check: 9247 from: 1 to: 5066 pF1KA0175 5066 bp With 182 enzymes: SgfI * July 16, 2019 14:21 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 831 1362 831 Size: 531 4535 AccI GT'mk_AC Cuts at: 2104 2104 Size: 5066 Acc65I G'GTAC_C Cuts at: 2082 2082 Size: 5066 AclI AA'CG_TT Cuts at: 4906 4906 Size: 5066 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1915 2891 3323 4070 4338 1915 Size: 976 432 747 268 2643 Fragments arranged by size: 2643 976 747 432 268 AflII C'TTAA_G Cuts at: 1999 1999 Size: 5066 AflIII A'CryG_T Cuts at: 2418 3522 4499 2418 Size: 1104 977 2985 Fragments arranged by size: 2985 1104 977 AgeI A'CCGG_T Cuts at: 3454 3454 Size: 5066 AhdI GACnn_n'nnGTC Cuts at: 1158 1158 Size: 5066 AleI CACnn'nnGTG Cuts at: 1896 1896 Size: 5066 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1079 1111 1079 Size: 32 5034 AlwNI CAG_nnn'CTG Cuts at: 637 1261 1530 2371 3938 637 Size: 624 269 841 1567 1765 Fragments arranged by size: 1765 1567 841 624 269 ApaI G_GGCC'C Cuts at: 1769 1769 Size: 5066 ApaLI G'TGCA_C Cuts at: 475 3836 475 Size: 3361 1705 ApoI r'AATT_y Cuts at: 516 1560 2070 4419 4675 516 Size: 1044 510 2349 256 907 Fragments arranged by size: 2349 1044 907 510 256 AseI AT'TA_AT Cuts at: 20 1219 20 Size: 1199 3867 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 5066 AvaI C'yCGr_G Cuts at: 434 1082 2086 434 Size: 648 1004 3414 Fragments arranged by size: 3414 1004 648 BamHI G'GATC_C Cuts at: 2091 2091 Size: 5066 BanI G'GyrC_C Cuts at: 1613 2082 2496 2744 2779 1613 Size: 469 414 248 35 3900 Fragments arranged by size: 3900 469 414 248 35 BanII G_rGCy'C Cuts at: 1769 2080 3110 1769 Size: 311 1030 3725 Fragments arranged by size: 3725 1030 311 BbeI G_GCGC'C Cuts at: 2500 2748 2500 Size: 248 4818 BbsI GAAGACnn'nnnn_ Cuts at: 435 1779 2042 435 Size: 1344 263 3459 Fragments arranged by size: 3459 1344 263 BciVI GTATCCnnnnn_n' Cuts at: 2964 3725 5066 2964 Size: 761 1341 2964 Fragments arranged by size: 2964 1341 761 BclI T'GATC_A Cuts at: 1038 2586 1038 Size: 1548 3518 BglI GCCn_nnn'nGGC Cuts at: 1078 1078 Size: 5066 BglII A'GATC_T Cuts at: 2581 4618 2581 Size: 2037 3029 BlpI GC'TnA_GC Cuts at: 2178 2178 Size: 5066 Bme1580I G_kGCm'C Cuts at: 479 1769 2691 2784 3840 479 Size: 1290 922 93 1056 1705 Fragments arranged by size: 1705 1290 1056 922 93 BmrI ACTGGGnnnn_n' Cuts at: 803 2278 2359 2694 4838 803 Size: 1475 81 335 2144 1031 Fragments arranged by size: 2144 1475 1031 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1495 3433 1495 Size: 1938 3128 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 227 398 1319 1430 227 Size: 171 921 111 3863 Fragments arranged by size: 3863 921 171 111 Bpu10I CC'TnA_GC Cuts at: 1433 1433 Size: 5066 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 385 490 2242 3121 3613 3911 385 Size: 105 1752 879 492 298 1540 Fragments arranged by size: 1752 1540 879 492 298 105 BsaI GGTCTCn'nnnn_ Cuts at: 424 568 4770 424 Size: 144 4202 720 Fragments arranged by size: 4202 720 144 BsaAI yAC'GTr Cuts at: 2419 2419 Size: 5066 BsaBI GATnn'nnATC Cuts at: 6 533 596 2580 4617 6 Size: 527 63 1984 2037 455 Fragments arranged by size: 2037 1984 527 455 63 BsaHI Gr'CG_yC Cuts at: 2497 2745 3447 4943 2497 Size: 248 702 1496 2620 Fragments arranged by size: 2620 1496 702 248 BsaWI w'CCGG_w Cuts at: 2255 2776 3454 3728 3875 4286 2255 Size: 521 678 274 147 411 3035 Fragments arranged by size: 3035 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 200 230 1667 1697 200 Size: 30 1437 30 3569 Fragments arranged by size: 3569 1437 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 395 442 1669 395 Size: 47 1227 3792 Fragments arranged by size: 3792 1227 47 BseYI C'CCAG_C Cuts at: 2491 3249 3826 4322 2491 Size: 758 577 496 3235 Fragments arranged by size: 3235 758 577 496 BsiEI CG_ry'CG Cuts at: 104 2608 2654 3862 104 Size: 2504 46 1208 1308 Fragments arranged by size: 2504 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 479 1730 2080 2858 3048 3840 479 Size: 1251 350 778 190 792 1705 Fragments arranged by size: 1705 1251 792 778 350 190 BsmI GAATG_Cn' Cuts at: 815 815 Size: 5066 BsmBI CGTCTCn'nnnn_ Cuts at: 1754 1754 Size: 5066 Bsp1286I G_dGCh'C Cuts at: 479 1730 1769 2080 2691 2784 2858 3048 3110 Size: 1251 39 311 611 93 74 190 62 Cuts at: 3110 3840 479 Size: 730 1705 Fragments arranged by size: 1705 1251 730 611 311 190 93 74 62 39 BspEI T'CCGG_A Cuts at: 4286 4286 Size: 5066 BspHI T'CATG_A Cuts at: 4242 4242 Size: 5066 BspMI ACCTGCnnnn'nnnn_ Cuts at: 831 1362 2116 2330 2632 3013 831 Size: 531 754 214 302 381 2884 Fragments arranged by size: 2884 754 531 381 302 214 BsrBI CCG'CTC Cuts at: 50 1619 3358 3412 4896 5061 50 Size: 1569 1739 54 1484 165 55 Fragments arranged by size: 1739 1569 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 2978 2978 Size: 5066 BsrFI r'CCGG_y Cuts at: 3064 3245 3454 3064 Size: 181 209 4676 Fragments arranged by size: 4676 209 181 BsrGI T'GTAC_A Cuts at: 981 1875 981 Size: 894 4172 BssSI C'ACGA_G Cuts at: 3337 3695 3337 Size: 358 4708 BstAPI GCAn_nnn'nTGC Cuts at: 2443 2443 Size: 5066 BstBI TT'CG_AA Cuts at: 3427 3427 Size: 5066 BstYI r'GATC_y Cuts at: 1 1632 2091 2558 2581 2916 3162 4174 4260 Size: 1631 459 467 23 335 246 1012 86 Cuts at: 4260 4272 4618 1 Size: 12 346 449 Fragments arranged by size: 1631 1012 467 459 449 346 335 246 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2021 3016 3197 3360 2021 Size: 995 181 163 3727 Fragments arranged by size: 3727 995 181 163 BtsI GCAGTG_nn' Cuts at: 2309 2309 Size: 5066 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 1295 1330 1295 Size: 35 5031 DraI TTT'AAA Cuts at: 2065 2065 Size: 5066 DrdI GACnn_nn'nnGTC Cuts at: 2772 3630 2772 Size: 858 4208 EaeI y'GGCC_r Cuts at: 2651 2825 3216 3243 4588 2651 Size: 174 391 27 1345 3129 Fragments arranged by size: 3129 1345 391 174 27 EagI C'GGCC_G Cuts at: 2651 2651 Size: 5066 EarI CTCTTCn'nnn_ Cuts at: 419 1409 3089 3299 419 Size: 990 1680 210 2186 Fragments arranged by size: 2186 1680 990 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3274 3584 3730 4876 3274 Size: 310 146 1146 3464 Fragments arranged by size: 3464 1146 310 146 EcoICRI GAG'CTC Cuts at: 2078 2078 Size: 5066 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 227 398 1319 1430 1915 2891 3323 4070 4338 Size: 171 921 111 485 976 432 747 268 Cuts at: 4338 227 Size: 955 Fragments arranged by size: 976 955 921 747 485 432 268 171 111 EcoO109I rG'GnC_Cy Cuts at: 501 2205 501 Size: 1704 3362 EcoRI G'AATT_C Cuts at: 2070 2070 Size: 5066 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 1127 3085 1127 Size: 1958 3108 FspI TGC'GCA Cuts at: 2847 2847 Size: 5066 HaeII r_GCGC'y Cuts at: 2500 2748 3770 4749 2500 Size: 248 1022 979 2817 Fragments arranged by size: 2817 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 403 435 1144 1176 1234 1266 2029 2061 2908 Size: 32 709 32 58 32 763 32 847 Cuts at: 2908 2938 2940 2970 403 Size: 30 2 30 2499 Fragments arranged by size: 2499 847 763 709 58 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 319 1869 2105 319 Size: 1550 236 3280 Fragments arranged by size: 3280 1550 236 HindIII A'AGCT_T Cuts at: 1780 1780 Size: 5066 KasI G'GCGC_C Cuts at: 2496 2744 2496 Size: 248 4818 KpnI G_GTAC'C Cuts at: 2086 2086 Size: 5066 MluI A'CGCG_T Cuts at: 4499 4499 Size: 5066 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1688 3737 3921 1688 Size: 2049 184 2833 Fragments arranged by size: 2833 2049 184 MscI TGG'CCA Cuts at: 2827 2827 Size: 5066 MslI CAynn'nnrTG Cuts at: 953 1790 1896 3055 3182 953 Size: 837 106 1159 127 2837 Fragments arranged by size: 2837 1159 837 127 106 MspA1I CmG'CkG Cuts at: 1995 2177 2491 2851 3864 4109 1995 Size: 182 314 360 1013 245 2952 Fragments arranged by size: 2952 1013 360 314 245 182 NaeI GCC'GGC Cuts at: 3247 3247 Size: 5066 NarI GG'CG_CC Cuts at: 2497 2745 2497 Size: 248 4818 NdeI CA'TA_TG Cuts at: 1649 1649 Size: 5066 NgoMIV G'CCGG_C Cuts at: 3245 3245 Size: 5066 NspI r_CATG'y Cuts at: 611 1867 2119 2619 3526 611 Size: 1256 252 500 907 2151 Fragments arranged by size: 2151 1256 907 500 252 PciI A'CATG_T Cuts at: 3522 3522 Size: 5066 PflMI CCAn_nnn'nTGG Cuts at: 1295 1295 Size: 5066 PmeI GTTT'AAAC Cuts at: 2065 2065 Size: 5066 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4205 4237 4205 Size: 32 5034 PpuMI rG'GwC_Cy Cuts at: 501 501 Size: 5066 PshAI GACnn'nnGTC Cuts at: 560 1591 560 Size: 1031 4035 PspOMI G'GGCC_C Cuts at: 1765 1765 Size: 5066 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 965 997 965 Size: 32 5034 PstI C_TGCA'G Cuts at: 842 2113 2798 842 Size: 1271 685 3110 Fragments arranged by size: 3110 1271 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 5066 PvuII CAG'CTG Cuts at: 2491 2851 2491 Size: 360 4706 RsrII CG'GwC_CG Cuts at: 3261 3261 Size: 5066 SacI G_AGCT'C Cuts at: 2080 2080 Size: 5066 SalI G'TCGA_C Cuts at: 2103 2103 Size: 5066 SapI GCTCTTCn'nnn_ Cuts at: 3089 3299 3089 Size: 210 4856 SbfI CC_TGCA'GG Cuts at: 2113 2113 Size: 5066 ScaI AGT'ACT Cuts at: 370 1294 1420 4583 370 Size: 924 126 3163 853 Fragments arranged by size: 3163 924 853 126 SfcI C'TryA_G Cuts at: 33 601 838 2109 2794 3787 3978 33 Size: 568 237 1271 685 993 191 1121 Fragments arranged by size: 1271 1121 993 685 568 237 191 SfoI GGC'GCC Cuts at: 2498 2746 2498 Size: 248 4818 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 5066 SmaI CCC'GGG Cuts at: 436 2088 436 Size: 1652 3414 SmlI C'TyrA_G Cuts at: 364 505 1999 2221 3136 3628 3890 364 Size: 141 1494 222 915 492 262 1540 Fragments arranged by size: 1540 1494 915 492 262 222 141 SpeI A'CTAG_T Cuts at: 67 67 Size: 5066 SphI G_CATG'C Cuts at: 2119 2619 2119 Size: 500 4566 SspI AAT'ATT Cuts at: 136 353 136 Size: 217 4849 StuI AGG'CCT Cuts at: 286 286 Size: 5066 StyI C'CwwG_G Cuts at: 578 2200 2554 578 Size: 1622 354 3090 Fragments arranged by size: 3090 1622 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 2649 2782 3241 3450 2649 Size: 133 459 209 4265 Fragments arranged by size: 4265 459 209 133 TatI w'GTAC_w Cuts at: 368 981 1292 1418 1875 4581 368 Size: 613 311 126 457 2706 853 Fragments arranged by size: 2706 853 613 457 311 126 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1667 1699 4583 4615 1667 Size: 32 2884 32 2118 Fragments arranged by size: 2884 2118 32 32 Tth111I GACn'n_nGTC Cuts at: 2863 2863 Size: 5066 XbaI T'CTAG_A Cuts at: 2097 2097 Size: 5066 XcmI CCAnnnn_n'nnnnTGG Cuts at: 202 2561 202 Size: 2359 2707 XmaI C'CCGG_G Cuts at: 434 2086 434 Size: 1652 3414 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PshAI PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AlfI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BsgI BsiWI BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NcoI NheI NotI NruI NsiI PacI PasI PfoI PmlI PsiI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI