################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AfaI AflII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvaI AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BseAI BsgI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DsaI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FbaI FokI FseI HaeII HincII HindII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboII MfeI MluI MluNI MroI MscI MunI MvnI NarI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcyI 513 GGCGTC G[GA]^CG[CT]C AhdI 73 GACATTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 258 GATCC GGATC(4/5) AspEI 73 GACATTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 592 GAGCAC G[AT]GC[AT]^C AvaII 468 GGACC G^G[AT]CC AviII 314 TGCGCA TGC^GCA BcnI 519 CCCGG CC^[GC]GG BglII 365 AGATCT A^GATCT BpmI 388 CTGGAG CTGGAG(16/14) BsaHI 513 GGCGTC G[GA]^CG[CT]C BsaWI 338 ACCGGA [AT]^CCGG[AT] BsiEI 336 CGACCG CG[GA][CT]^CG BsiHKAI 592 GAGCAC G[AT]GC[AT]^C BsrFI 211 GCCGGC [GA]^CCGG[CT] BsrI 527 CCAGT ACTGG(1/-1) BstAPI 550 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 580 CCTGG CC^[AT]GG BstXI 567 CCATGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 365 AGATCT [GA]^GATC[CT] Cfr10I 211 GCCGGC [GA]^CCGG[CT] DrdI 135 GACGCAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 73 GACATTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRII 580 CCTGG ^CC[AT]GG EcoT22I 181 ATGCAT ATGCA^T FspI 314 TGCGCA TGC^GCA Hin1I 513 GGCGTC G[GA]^CG[CT]C HindIII 452 AAGCTT A^AGCTT MaeIII 144 GTCAC ^GT[ACGT]AC MflI 365 AGATCT [GA]^GATC[CT] MseI 77 TTAA T^TAA MspA1I 310 CAGCTG C[AC]G^C[GT]G MvaI 580 CCTGG CC^[AT]GG NaeI 211 GCCGGC GCC^GGC NciI 519 CCCGG CC^[GC]GG NgoMIV 211 GCCGGC G^CCGGC NsiI 181 ATGCAT ATGCA^T NspI 183 GCATGT [GA]CATG^[CT] PvuII 310 CAGCTG CAG^CTG SfaNI 180 GATGC GCATC(5/9) TaqI 473 TCGA T^CGA Tru9I 77 TTAA T^TAA Tsp45I 144 GTCAC ^GT[GC]AC Tsp509I 172 AATT ^AATT TthHB8I 473 TCGA T^CGA XcmI 561 CCACTCCCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 365 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### BanII 14 GAGCCC G[GA]GC[CT]^C 523 GAGCCC BsaJI 518 CCCCGG C^C[ACGT][ACGT]GG 579 CCCTGG BsmFI 219 GTCCC GGGAC(10/14) 516 GTCCC BspMI 437 ACCTGC ACCTGC(4/8) 138 GCAGGT BssKI 519 CCCGG ^CC[ACGT]GG 580 CCTGG CfoI 300 GCGC GCG^C 315 GCGC DdeI 155 CTCAG C^T[ACGT]AG 590 CTGAG HhaI 300 GCGC GCG^C 315 GCGC HinP1I 300 GCGC G^CGC 315 GCGC HinfI 217 GAGTC G^A[ACGT]TC 481 GACTC HphI 160 GGTGA GGTGA(8/7) 98 TCACC MslI 562 CACTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 568 CATGCTGGTG PleI 481 GACTC GAGTC(4/5) 217 GAGTC PstI 43 CTGCAG CTGCA^G 322 CTGCAG ScrFI 519 CCCGG CC^[ACGT]GG 580 CCTGG SmlI 244 CTCAAG C^T[CT][GA]AG 414 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AluI AlwNI BbvI BmyI BseRI BsiYI BslI BsmAI BsmBI Bsp1286I Cac8I Cfr13I DpnII Fnu4HI HaeIII HapII HgaI HpaII ItaI MboI MnlI MspI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I SfcI TseI TspRI