################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I CpoI DraII DraIII DrdI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AhdI 786 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 218 GTGCAC G^TGCAC ApaLI 218 GTGCAC G^TGCAC AspEI 786 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BcnI 613 CCCGG CC^[GC]GG BsaAI 628 CACGTA [CT]AC^GT[GA] BsaBI 559 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 193 GCATTC GAATGC(1/-1) BspDI 163 ATCGAT AT^CGAT BstYI 425 AGATCC [GA]^GATC[CT] ClaI 163 ATCGAT AT^CGAT DraI 619 TTTAAA TTT^AAA Eam1105I 786 GACAGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 304 GAAGAG CTCTTC(1/4) EcoNI 369 CCTACAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 683 GAATTC G^AATTC EcoT14I 1010 CCATGG C^C[AT][AT]GG HaeIII 768 GGCC GG^CC HapII 614 CCGG C^CGG HindIII 888 AAGCTT A^AGCTT HinfI 1006 GAATC G^A[ACGT]TC HpaII 614 CCGG C^CGG Ksp632I 304 GAAGAG CTCTTC(1/4) MamI 559 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 425 AGATCC [GA]^GATC[CT] MspA1I 33 CAGCGG C[AC]G^C[GT]G MspI 614 CCGG C^CGG NciI 613 CCCGG CC^[GC]GG NcoI 1010 CCATGG C^CATGG NlaIV 44 GGAACC GG[ACGT]^[ACGT]CC NspI 713 GCATGC [GA]CATG^[CT] SfcI 740 CTGTAG C^T[GA][CT]AG SmlI 832 CTTGAG C^T[CT][GA]AG SphI 713 GCATGC GCATG^C StyI 1010 CCATGG C^C[AT][AT]GG TfiI 1006 GAATC G^A[AT]TC XbaI 640 TCTAGA T^CTAGA XhoII 425 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AciI 35 GCGG CCGC(-3/-1) 777 GCGG AlwI 426 GATCC GGATC(4/5) 436 GATCC AspHI 85 GTGCTC G[AT]GC[AT]^C 218 GTGCAC AvaI 27 CCCGAG C^[CT]CG[GA]G 77 CCCGAG AvaII 661 GGACC G^G[AT]CC 793 GGTCC BfaI 641 CTAG C^TAG 671 CTAG BpmI 178 CTCCAG CTGGAG(16/14) 49 CTGGAG BseRI 982 CTCCTC GAGGAG(10/8) 283 GAGGAG BsiHKAI 85 GTGCTC G[AT]GC[AT]^C 218 GTGCAC BsoBI 27 CCCGAG C^[CT]CG[GA]G 77 CCCGAG BsrDI 206 CATTGC GCAATG(2/0) 548 GCAATG BsrI 939 ACTGG ACTGG(1/-1) 796 CCAGT DdeI 136 CTCAG C^T[ACGT]AG 985 CTCAG DpnII 426 GATC ^GATC 436 GATC DsaI 11 CCACGG C^C[GA][CT]GG 1010 CCATGG HphI 37 GGTGA GGTGA(8/7) 150 GGTGA MaeI 641 CTAG C^TAG 671 CTAG MaeII 533 ACGT A^CGT 629 ACGT MboI 426 GATC ^GATC 436 GATC NdeII 426 GATC ^GATC 436 GATC NlaIII 714 CATG CATG^ 1011 CATG Sau3AI 426 GATC ^GATC 436 GATC SspI 760 AATATT AAT^ATT 872 AATATT TaqI 164 TCGA T^CGA 720 TCGA Tsp45I 38 GTGAC ^GT[GC]AC 626 GTCAC TspRI 797 CAGTG CA[GC]TG[ACGT][ACGT]^ 938 CACTG TthHB8I 164 TCGA T^CGA 720 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AcsI AluI ApoI Asp700I BbvI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BssKI BstNI Cac8I Cfr13I EcoRII Fnu4HI ItaI MaeIII MboII MnlI MseI MvaI MwoI Sau96I ScrFI SfaNI Tru9I TseI Tsp509I XmnI