Restriction map for pF1KB6529
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AccI        AclI        AcsI        AgeI        Aor51HI     
ApaI        ApoI        AscI        AseI        AsnI        
AspI        AvrII       BamHI       BbrPI       BcgI        
BciVI       BclI        BlnI        BsaBI       BsaI        
BsiWI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       Bst1107I    
BstAPI      BstBI       BstZ17I     Cfr10I      ClaI        
CpoI        DraI        DraIII      Eco47III    EcoNI       
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        HindIII     HpaI        KspI        MamI        
MfeI        MluI        MunI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StyI        
SwaI        Tsp509I     Tth111I     XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   72 enzymes) #
###################################

AatII          37  GACGTC               GACGT^C
Acc65I        560  GGTACC               G^GTACC
AccIII         11  TCCGGA               T^CCGGA
AflII         412  CTTAAG               C^TTAAG
AflIII         74  ACGTGT               A^C[GA][CT]GT
Alw44I        439  GTGCAC               G^TGCAC
AocI          940  CCTGAGG              CC^T[ACGT]AGG
ApaLI         439  GTGCAC               G^TGCAC
Asp700I       801  GAAGGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       560  GGTACC               G^GTACC
AvaI          571  CCCGAG               C^[CT]CG[GA]G
AviII         290  TGCGCA               TGC^GCA
BalI          152  TGGCCA               TGG^CCA
BbeI         1073  GGCGCC               GGCGC^C
BbsI          268  GTCTTC               GAAGAC(2/6)
BbvCI         582  CCTCAGC              CCTCAGC(-5/-2)
BfaI          871  CTAG                 C^TAG
BfrI          412  CTTAAG               C^TTAAG
BglII         188  AGATCT               A^GATCT
BlpI          165  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      165  GCTGAGC              GC^T[ACGT]AGC
BpuAI         268  GTCTTC               GAAGAC(2/6)
BsaWI          11  TCCGGA               [AT]^CCGG[AT]
BseAI          11  TCCGGA               T^CCGGA
BsgI          739  GTGCAG               GTGCAG(16/14)
BsiEI         893  CGGCCG               CG[GA][CT]^CG
BsmAI         949  GAGAC                GTCTC(1/5)
BsmBI         949  GAGACG               CGTCTC(1/5)
BsoBI         571  CCCGAG               C^[CT]CG[GA]G
BspEI          11  TCCGGA               T^CCGGA
BspMI         848  ACCTGC               ACCTGC(4/8)
BsrDI        1053  CATTGC               GCAATG(2/0)
BssHII        201  GCGCGC               G^CGCGC
BssSI         988  CACGAG               CACGAG(-5/-1)
BstEII        549  GGTGACC              G^GT[ACGT]ACC
BstPI         549  GGTGACC              G^GT[ACGT]ACC
BstXI         423  CCACTCTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        940  CCTGAGG              CC^T[ACGT]AGG
CelII         165  GCTGAGC              GC^T[ACGT]AGC
DrdI           69  GACCTACGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          823  CCACGG               C^C[GA][CT]GG
EagI          893  CGGCCG               C^GGCCG
EarI          946  GAAGAG               CTCTTC(1/4)
EclXI         893  CGGCCG               C^GGCCG
Eco52I        893  CGGCCG               C^GGCCG
Eco81I        940  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       549  GGTGACC              G^GT[ACGT]ACC
FspI          290  TGCGCA               TGC^GCA
HincII         40  GTCAAC               GT[CT]^[GA]AC
HindII         40  GTCAAC               GT[CT]^[GA]AC
KasI         1073  GGCGCC               G^GCGCC
KpnI          560  GGTACC               GGTAC^C
Ksp632I       946  GAAGAG               CTCTTC(1/4)
MaeI          871  CTAG                 C^TAG
MluNI         152  TGGCCA               TGG^CCA
MroI           11  TCCGGA               T^CCGGA
MscI          152  TGGCCA               TGG^CCA
MseI          413  TTAA                 T^TAA
MslI          351  CATGGGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI         1073  GGCGCC               GG^CGCC
PleI         1066  GACTC                GAGTC(4/5)
PshAI          84  GACGCACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          340  CTGCAG               CTGCA^G
SfcI          340  CTGCAG               C^T[GA][CT]AG
SfiI          894  GGCCGCGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1073  GGCGCC               GGC^GCC
SmlI          412  CTTAAG               C^T[CT][GA]AG
StuI          408  AGGCCT               AGG^CCT
TaqI          128  TCGA                 T^CGA
Tru9I         413  TTAA                 T^TAA
TthHB8I       128  TCGA                 T^CGA
XmnI          801  GAAGGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AhdI          130  GACAAGCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              613  GACATCTGGTC         
AspEI         130  GACAAGCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              613  GACATCTGGTC         
AspHI         117  GTGCTC               G[AT]GC[AT]^C
              439  GTGCAC              
BanII         256  GGGCTC               G[GA]GC[CT]^C
              566  GAGCCC              
BglI          895  GCCGCGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1076  GCCGGAGTGGC         
BpmI          853  CTGGAG               CTGGAG(16/14)
              865  CTGGAG              
BsaAI          73  TACGTG               [CT]AC^GT[GA]
              544  TACGTG              
BseRI         282  CTCCTC               GAGGAG(10/8)
              370  GAGGAG              
BsiHKAI       117  GTGCTC               G[AT]GC[AT]^C
              439  GTGCAC              
Eam1105I      130  GACAAGCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              613  GACATCTGGTC         
FauI          554  CCCGC                CCCGC(4/6)
               98  GCGGG               
HaeII         884  AGCGCC               [GA]GCGC^[CT]
             1073  GGCGCC              
HgaI           84  GACGC                GACGC(5/10)
              526  GACGC               
MaeIII        550  GTGAC                ^GT[ACGT]AC
              712  GTGAC               
NspI          233  ACATGC               [GA]CATG^[CT]
              519  ACATGC              
PflMI         381  CCAGTACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              603  CCAGACAGTGG         
PpuMI         446  GGGACCT              [GA]G^G[AT]CC[CT]
              935  GGGACCC             
SexAI         311  ACCTGGT              A^CC[AT]GGT
              386  ACCTGGT             
TfiI          495  GATTC                G^A[AT]TC
              976  GATTC               
Tsp45I        550  GTGAC                ^GT[GC]AC
              712  GTGAC               
Van91I        381  CCAGTACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              603  CCAGACAGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AvaII       BanI        BbvI        
BcnI        BmyI        BsaHI       BsaJI       BsiYI       
BslI        BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        MaeII       
MboI        MboII       MflI        MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       TseI        
TspRI       XhoII       
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