Restriction map for pF1KB8452
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     AspI        AviII       
AvrII       BbrPI       BcgI        BciVI       BfrI        
BglII       BlnI        BsaBI       BseAI       BseRI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrDI       BsrGI       Bst1107I    
BstBI       BstXI       BstZ17I     Bsu36I      ClaI        
CpoI        DraI        DraIII      DrdI        EarI        
Eco47III    Eco81I      EcoNI       EcoRI       EcoRV       
EcoT22I     FseI        FspI        HindIII     HpaI        
Ksp632I     KspI        MamI        MfeI        MluI        
MroI        MseI        MunI        NaeI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SfiI        SfuI        SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TfiI        Tru9I       Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I        539  GGTACC               G^GTACC
AhdI          592  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I       539  GGTACC               G^GTACC
AspEI         592  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI         386  GGATCC               G^GATCC
BbeI          106  GGCGCC               GGCGC^C
BbsI          457  GAAGAC               GAAGAC(2/6)
BbvCI         420  GCTGAGG              CCTCAGC(-5/-2)
BclI          626  TGATCA               T^GATCA
BfaI          373  CTAG                 C^TAG
BlpI         1014  GCTCAGC              GC^T[ACGT]AGC
BpmI          832  CTGGAG               CTGGAG(16/14)
Bpu1102I     1014  GCTCAGC              GC^T[ACGT]AGC
BpuAI         457  GAAGAC               GAAGAC(2/6)
BsaAI         523  TACGTG               [CT]AC^GT[GA]
BsaWI         383  ACCGGA               [AT]^CCGG[AT]
BsmBI        1060  GAGACG               CGTCTC(1/5)
BspMI          86  ACCTGC               ACCTGC(4/8)
BsrFI         145  ACCGGC               [GA]^CCGG[CT]
BstAPI        720  GCAGCGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        1014  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        145  ACCGGC               [GA]^CCGG[CT]
Eam1105I      592  GACATCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I      1055  CCAAGG               C^C[AT][AT]GG
FbaI          626  TGATCA               T^GATCA
HincII        967  GTTGAC               GT[CT]^[GA]AC
HindII        967  GTTGAC               GT[CT]^[GA]AC
KasI          106  GGCGCC               G^GCGCC
KpnI          539  GGTACC               GGTAC^C
MaeI          373  CTAG                 C^TAG
MslI          684  CATGAAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          106  GGCGCC               GG^CGCC
NspI          239  ACATGC               [GA]CATG^[CT]
SacI            3  GAGCTC               GAGCT^C
SexAI         317  ACCTGGT              A^CC[AT]GGT
SfoI          106  GGCGCC               GGC^GCC
SgrAI         144  CACCGGCG             C[GA]^CCGG[CT]G
SmlI          232  CTCAAG               C^T[CT][GA]AG
StyI         1055  CCAAGG               C^C[AT][AT]GG
TaqI          902  TCGA                 T^CGA
TthHB8I       902  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AlwNI         406  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              721  CAGCGGCTG           
AvaI          102  CTCGGG               C^[CT]CG[GA]G
              550  CCCGAG              
BalI          158  TGGCCA               TGG^CCA
              491  TGGCCA              
BsaI          289  GAGACC               GGTCTC(1/5)
             1050  GGTCTC              
BsgI          910  CTGCAC               GTGCAG(16/14)
              718  GTGCAG              
BsiEI         128  CGGCCG               CG[GA][CT]^CG
              138  CGGCCG              
BsoBI         102  CTCGGG               C^[CT]CG[GA]G
              550  CCCGAG              
BspHI         356  TCATGA               T^CATGA
              683  TCATGA              
BssHII         69  GCGCGC               G^CGCGC
              207  GCGCGC              
BssSI         247  CACGAG               CACGAG(-5/-1)
              397  CTCGTG              
BstEII         45  GGTGACC              G^GT[ACGT]ACC
              528  GGTGACC             
BstPI          45  GGTGACC              G^GT[ACGT]ACC
              528  GGTGACC             
BstYI         386  GGATCC               [GA]^GATC[CT]
              473  AGATCC              
EagI          128  CGGCCG               C^GGCCG
              138  CGGCCG              
EclXI         128  CGGCCG               C^GGCCG
              138  CGGCCG              
Eco52I        128  CGGCCG               C^GGCCG
              138  CGGCCG              
EcoO65I        45  GGTGACC              G^GT[ACGT]ACC
              528  GGTGACC             
HinfI         904  GAGTC                G^A[ACGT]TC
              952  GACTC               
MflI          386  GGATCC               [GA]^GATC[CT]
              473  AGATCC              
MluNI         158  TGGCCA               TGG^CCA
              491  TGGCCA              
MscI          158  TGGCCA               TGG^CCA
              491  TGGCCA              
PleI          952  GACTC                GAGTC(4/5)
              904  GAGTC               
PpuMI          83  GGGACCT              [GA]G^G[AT]CC[CT]
              425  GGGACCT             
PstI           88  CTGCAG               CTGCA^G
              727  CTGCAG              
PvuII         670  CAGCTG               CAG^CTG
             1036  CAGCTG              
RcaI          356  TCATGA               T^CATGA
              683  TCATGA              
SfcI           88  CTGCAG               C^T[GA][CT]AG
              727  CTGCAG              
Tsp509I       565  AATT                 ^AATT
              770  AATT                
XhoII         386  GGATCC               [GA]^GATC[CT]
              473  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AspHI       AvaII       BanI        BanII       
BbvI        BcnI        BglI        BmyI        BsaHI       
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HpaII       HphI        ItaI        
MaeII       MaeIII      MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       TseI        
Tsp45I      TspRI       
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