Restriction map for pF1KB8452
 (Linear) (Six-base) MAPSORT of: KIBB8452.seq  Check: 1023  from: 1  to: 1071



KIBB8452.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

Acc65I G'GTAC_C

Cuts at:      0     539    1071 
   Size:        539     532
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     433     451     490     694    1071 
   Size:        433      18      39     204     377

  Fragments arranged by size: 

                 433     377     204      39      18
 
AhdI GACnn_n'nnGTC

Cuts at:      0     597    1071 
   Size:        597     474
 
AlwNI CAG_nnn'CTG

Cuts at:      0     411     726    1071 
   Size:        411     315     345

  Fragments arranged by size: 

                 411     345     315
 
AvaI C'yCGr_G

Cuts at:      0     102     550    1071 
   Size:        102     448     521

  Fragments arranged by size: 

                 521     448     102
 
BamHI G'GATC_C

Cuts at:      0     386    1071 
   Size:        386     685
 
BanI G'GyrC_C

Cuts at:      0     106     337     539    1071 
   Size:        106     231     202     532

  Fragments arranged by size: 

                 532     231     202     106
 
BanII G_rGCy'C

Cuts at:      0       7     103     549     932    1071 
   Size:          7      96     446     383     139

  Fragments arranged by size: 

                 446     383     139      96       7
 
BbeI G_GCGC'C

Cuts at:      0     110    1071 
   Size:        110     961
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     464    1071 
   Size:        464     607
 
BbvCI CC'TCA_GC

Cuts at:      0     421    1071 
   Size:        421     650
 
BclI T'GATC_A

Cuts at:      0     626    1071 
   Size:        626     445
 
BglI GCCn_nnn'nGGC

Cuts at:      0     146     499    1032    1071 
   Size:        146     353     533      39

  Fragments arranged by size: 

                 533     353     146      39
 
BlpI GC'TnA_GC

Cuts at:      0    1015    1071 
   Size:       1015      56
 
Bme1580I G_kGCm'C

Cuts at:      0     340    1071 
   Size:        340     731
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     527    1071 
   Size:        527     544
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     853    1071 
   Size:        853     218
 
Bpu10I CC'TnA_GC

Cuts at:      0     152     421    1071 
   Size:        152     269     650

  Fragments arranged by size: 

                 650     269     152
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217    1071 
   Size:        217     854
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     283    1056    1071 
   Size:        283     773      15

  Fragments arranged by size: 

                 773     283      15
 
BsaAI yAC'GTr

Cuts at:      0     525    1071 
   Size:        525     546
 
BsaHI Gr'CG_yC

Cuts at:      0     107     700    1063    1071 
   Size:        107     593     363       8

  Fragments arranged by size: 

                 593     363     107       8
 
BsaWI w'CCGG_w

Cuts at:      0     383    1071 
   Size:        383     688
 
BseYI C'CCAG_C

Cuts at:      0    1038    1071 
   Size:       1038      33
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     739     895    1071 
   Size:        739     156     176

  Fragments arranged by size: 

                 739     176     156
 
BsiEI CG_ry'CG

Cuts at:      0     131     141    1071 
   Size:        131      10     930

  Fragments arranged by size: 

                 930     131      10
 
BsiHKAI G_wGCw'C

Cuts at:      0       7     127    1016    1071 
   Size:          7     120     889      55

  Fragments arranged by size: 

                 889     120      55       7
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1054    1071 
   Size:       1054      17
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     103     127     340     549     932    1016    1071 
   Size:          7      96      24     213     209     383      84      55

  Fragments arranged by size: 

                 383     213     209      96      84      55      24       7
            
 
BspHI T'CATG_A

Cuts at:      0     356     683    1071 
   Size:        356     327     388

  Fragments arranged by size: 

                 388     356     327
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      95    1071 
   Size:         95     976
 
BsrFI r'CCGG_y

Cuts at:      0     145    1071 
   Size:        145     926
 
BssHII G'CGCG_C

Cuts at:      0      69     207    1071 
   Size:         69     138     864

  Fragments arranged by size: 

                 864     138      69
 
BssSI C'ACGA_G

Cuts at:      0     247     397    1071 
   Size:        247     150     674

  Fragments arranged by size: 

                 674     247     150
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     726    1071 
   Size:        726     345
 
BstEII G'GTnAC_C

Cuts at:      0      45     528    1071 
   Size:         45     483     543

  Fragments arranged by size: 

                 543     483      45
 
BstYI r'GATC_y

Cuts at:      0     386     473    1071 
   Size:        386      87     598

  Fragments arranged by size: 

                 598     386      87
 
BtgI C'CryG_G

Cuts at:      0      95     131     602    1071 
   Size:         95      36     471     469

  Fragments arranged by size: 

                 471     469      95      36
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     749    1071 
   Size:        749     322
 
BtsI GCAGTG_nn'

Cuts at:      0      30    1071 
   Size:         30    1041
 
EaeI y'GGCC_r

Cuts at:      0     128     138     158     491    1071 
   Size:        128      10      20     333     580

  Fragments arranged by size: 

                 580     333     128      20      10
 
EagI C'GGCC_G

Cuts at:      0     128     138    1071 
   Size:        128      10     933

  Fragments arranged by size: 

                 933     128      10
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     634    1071 
   Size:        634     437
 
EcoICRI GAG'CTC

Cuts at:      0       5    1071 
   Size:          5    1066
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     433     451     490     694     853    1071 
   Size:        433      18      39     204     159     218

  Fragments arranged by size: 

                 433     218     204     159      39      18
 
EcoO109I rG'GnC_Cy

Cuts at:      0      84     426     759    1071 
   Size:         84     342     333     312

  Fragments arranged by size: 

                 342     333     312      84
 
HaeII r_GCGC'y

Cuts at:      0     110     152     886    1071 
   Size:        110      42     734     185

  Fragments arranged by size: 

                 734     185     110      42
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     583     615     775     807    1071 
   Size:        583      32     160      32     264

  Fragments arranged by size: 

                 583     264     160      32      32
 
HincII GTy'rAC

Cuts at:      0     969    1071 
   Size:        969     102
 
KasI G'GCGC_C

Cuts at:      0     106    1071 
   Size:        106     965
 
KpnI G_GTAC'C

Cuts at:      0     543    1071 
   Size:        543     528
 
MscI TGG'CCA

Cuts at:      0     160     493    1071 
   Size:        160     333     578

  Fragments arranged by size: 

                 578     333     160
 
MslI CAynn'nnrTG

Cuts at:      0     688    1071 
   Size:        688     383
 
MspA1I CmG'CkG

Cuts at:      0     672     723     864    1038    1071 
   Size:        672      51     141     174      33

  Fragments arranged by size: 

                 672     174     141      51      33
 
NarI GG'CG_CC

Cuts at:      0     107    1071 
   Size:        107     964
 
NspI r_CATG'y

Cuts at:      0     243    1071 
   Size:        243     828
 
PfoI T'CCnGG_A

Cuts at:      0     476     830    1071 
   Size:        476     354     241

  Fragments arranged by size: 

                 476     354     241
 
PpuMI rG'GwC_Cy

Cuts at:      0      84     426    1071 
   Size:         84     342     645

  Fragments arranged by size: 

                 645     342      84
 
PstI C_TGCA'G

Cuts at:      0      92     731    1071 
   Size:         92     639     340

  Fragments arranged by size: 

                 639     340      92
 
PvuII CAG'CTG

Cuts at:      0     672    1038    1071 
   Size:        672     366      33

  Fragments arranged by size: 

                 672     366      33
 
SacI G_AGCT'C

Cuts at:      0       7    1071 
   Size:          7    1064
 
SexAI A'CCwGG_T

Cuts at:      0     317    1071 
   Size:        317     754
 
SfcI C'TryA_G

Cuts at:      0      88     727    1071 
   Size:         88     639     344

  Fragments arranged by size: 

                 639     344      88
 
SfoI GGC'GCC

Cuts at:      0     108    1071 
   Size:        108     963
 
SgrAI Cr'CCGG_yG

Cuts at:      0     145    1071 
   Size:        145     926
 
SmlI C'TyrA_G

Cuts at:      0     232    1071 
   Size:        232     839
 
StyI C'CwwG_G

Cuts at:      0    1055    1071 
   Size:       1055      16
 

 Enzymes that do cut:

   Acc65I     AcuI     AhdI    AlwNI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BclI     BglI     BlpI Bme1580I     BmrI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrFI
   BssHII    BssSI   BstAPI   BstEII    BstYI     BtgI    BtgZI     BtsI
     EaeI     EagI     EciI  EcoICRI  Eco57MI EcoO109I    HaeII    Hin4I
   HincII     KasI     KpnI     MscI     MslI   MspA1I     NarI     NspI
     PfoI    PpuMI     PstI    PvuII     SacI    SexAI     SfcI     SfoI
    SgrAI     SmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI     BcgI    BciVI    BfrBI    BglII    BmgBI
     BmtI     BplI    BsaBI    BsaXI    BseRI    BsiWI     BsmI    BspEI
    BsrBI    BsrDI    BsrGI    BstBI    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI     DraI   DraIII     DrdI     EarI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI  HindIII     HpaI     MfeI     MluI
     MmeI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SgfI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
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