Restriction map for pF1KB7339
##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AccI        AflII       AflIII      AgeI        Aor51HI     
AscI        AseI        AsnI        AspI        AviII       
AvrII       BbeI        BbrPI       BbsI        BcgI        
BclI        BfrI        BglI        BglII       BlnI        
BpuAI       BsaBI       BsiWI       BsmI        Bsp1407I    
BspDI       BspLU11I    BsrDI       BsrGI       BssSI       
Bst1107I    BstBI       BstZ17I     ClaI        CpoI        
DraI        Eco47III    EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KasI        KspI        MamI        MfeI        
MluI        MunI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PshBI       PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
XbaI        XcmI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I       1907  GGTACC               G^GTACC
AclI         1648  AACGTT               AA^CGTT
Alw44I        652  GTGCAC               G^TGCAC
AocI         1089  CCTCAGG              CC^T[ACGT]AGG
ApaLI         652  GTGCAC               G^TGCAC
Asp700I      1920  GAACAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1907  GGTACC               G^GTACC
BalI          497  TGGCCA               TGG^CCA
BanI         1907  GGTACC               G^G[CT][GA]CC
BbvCI        2465  CCTCAGC              CCTCAGC(-5/-2)
BlpI         1257  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1257  GCTGAGC              GC^T[ACGT]AGC
BsmBI        1882  GAGACG               CGTCTC(1/5)
BspHI         677  TCATGA               T^CATGA
BsrFI         206  GCCGGC               [GA]^CCGG[CT]
BssHII       2081  GCGCGC               G^CGCGC
BstAPI       2304  GCATGGAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       1089  CCTCAGG              CC^T[ACGT]AGG
CelII        1257  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        206  GCCGGC               [GA]^CCGG[CT]
DraIII       2065  CACACGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1962  GACGCCTCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1089  CCTCAGG              CC^T[ACGT]AGG
EcoNI         174  CCTTCAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         274  GAATTC               G^AATTC
FauI         2011  CCCGC                CCCGC(4/6)
HaeII         790  AGCGCC               [GA]GCGC^[CT]
KpnI         1907  GGTACC               GGTAC^C
MluNI         497  TGGCCA               TGG^CCA
MscI          497  TGGCCA               TGG^CCA
MseI           95  TTAA                 T^TAA
NaeI          206  GCCGGC               GCC^GGC
NcoI          231  CCATGG               C^CATGG
NgoMIV        206  GCCGGC               G^CCGGC
PflMI         366  CCAGGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I     1648  AACGTT               AA^CGTT
PstI         1166  CTGCAG               CTGCA^G
RcaI          677  TCATGA               T^CATGA
SapI         2058  GCTCTTC              GCTCTTC(1/4)
SexAI         122  ACCTGGT              A^CC[AT]GGT
Tru9I          95  TTAA                 T^TAA
Van91I        366  CCAGGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         1920  GAACAAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AatII           4  GACGTC               GACGT^C
             1884  GACGTC              
AccII        1980  CGCG                 CG^CG
             2082  CGCG                
AccIII        540  TCCGGA               T^CCGGA
             1487  TCCGGA              
AcsI          274  GAATTC               [GA]^AATT[CT]
             1924  AAATTC              
AfaI         1635  GTAC                 GT^AC
             1908  GTAC                
ApaI         1291  GGGCCC               GGGCC^C
             1857  GGGCCC              
ApoI          274  GAATTC               [GA]^AATT[CT]
             1924  AAATTC              
BamHI         983  GGATCC               G^GATCC
             1490  GGATCC              
BciVI         571  GGATAC               GTATCC(6/5)
              820  GTATCC              
BfaI         1616  CTAG                 C^TAG
             2326  CTAG                
BsaAI          78  CACGTA               [CT]AC^GT[GA]
             1626  TACGTG              
BseAI         540  TCCGGA               T^CCGGA
             1487  TCCGGA              
BsiEI        1502  CGGCCG               CG[GA][CT]^CG
             2041  CGGCCG              
BspEI         540  TCCGGA               T^CCGGA
             1487  TCCGGA              
BstEII       2136  GGTGACC              G^GT[ACGT]ACC
             2193  GGTGACC             
BstPI        2136  GGTGACC              G^GT[ACGT]ACC
             2193  GGTGACC             
BstUI        1980  CGCG                 CG^CG
             2082  CGCG                
DsaI          231  CCATGG               C^C[GA][CT]GG
             2264  CCGTGG              
EagI         1502  CGGCCG               C^GGCCG
             2041  CGGCCG              
EclXI        1502  CGGCCG               C^GGCCG
             2041  CGGCCG              
Eco52I       1502  CGGCCG               C^GGCCG
             2041  CGGCCG              
EcoO65I      2136  GGTGACC              G^GT[ACGT]ACC
             2193  GGTGACC             
HgaI          503  GACGC                GACGC(5/10)
             1962  GACGC               
MaeI         1616  CTAG                 C^TAG
             2326  CTAG                
MroI          540  TCCGGA               T^CCGGA
             1487  TCCGGA              
MslI          795  CACCAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2022  CATCGGCGTG          
MvnI         1980  CGCG                 CG^CG
             2082  CGCG                
PaeR7I        101  CTCGAG               C^TCGAG
             1082  CTCGAG              
PleI          771  GAGTC                GAGTC(4/5)
             1123  GAGTC               
PshAI         997  GACTGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1222  GACGAGGGTC          
RsaI         1635  GTAC                 GT^AC
             1908  GTAC                
SacI          331  GAGCTC               GAGCT^C
             1848  GAGCTC              
SmlI          101  CTCGAG               C^T[CT][GA]AG
             1082  CTCGAG              
TfiI          837  GAATC                G^A[AT]TC
              876  GAATC               
XhoI          101  CTCGAG               C^TCGAG
             1082  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   93 enzymes) #
#########################################

AciI        AcyI        AhdI        AluI        AlwI        
AlwNI       AspEI       AspHI       AvaI        AvaII       
BanII       BbvI        BcnI        BmyI        BpmI        
BsaHI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        Eam1105I    EarI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PpuMI       
PvuII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        StuI        StyI        TaqI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmaCI       XmaI        
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