Restriction map for pF1KB7339
 (Linear) (Six-base) MAPSORT of: KIBB7339.seq  Check: 1632  from: 1  to: 2499



KIBB7339.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1547    1932    2124    2304    2499 
   Size:       1547     385     192     180     195

  Fragments arranged by size: 

                1547     385     195     192     180
 
AatII G_ACGT'C

Cuts at:      0       8    1888    2499 
   Size:          8    1880     611

  Fragments arranged by size: 

                1880     611       8
 
Acc65I G'GTAC_C

Cuts at:      0    1907    2499 
   Size:       1907     592
 
AclI AA'CG_TT

Cuts at:      0    1649    2499 
   Size:       1649     850
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     160     583     730     892    1885    2272    2499 
   Size:        154       6     423     147     162     993     387     227

  Fragments arranged by size: 

                 993     423     387     227     162     154     147       6
            
 
AhdI GACnn_n'nnGTC

Cuts at:      0     111     594     770    2499 
   Size:        111     483     176    1729

  Fragments arranged by size: 

                1729     483     176     111
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1369    1401    1774    1806    2499 
   Size:       1369      32     373      32     693

  Fragments arranged by size: 

                1369     693     373      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      66     372    1539    1947    2002    2352    2499 
   Size:         66     306    1167     408      55     350     147

  Fragments arranged by size: 

                1167     408     350     306     147      66      55
 
ApaI G_GGCC'C

Cuts at:      0    1295    1861    2499 
   Size:       1295     566     638

  Fragments arranged by size: 

                1295     638     566
 
ApaLI G'TGCA_C

Cuts at:      0     652    2499 
   Size:        652    1847
 
ApoI r'AATT_y

Cuts at:      0     274    1924    2499 
   Size:        274    1650     575

  Fragments arranged by size: 

                1650     575     274
 
AvaI C'yCGr_G

Cuts at:      0      38     101     880     922    1082    1263    1388    2035 
   Size:         38      63     779      42     160     181     125     647

Cuts at:   2035    2474    2499 
   Size:        439      25

  Fragments arranged by size: 

                 779     647     439     181     160     125      63      42
                  38      25
 
BamHI G'GATC_C

Cuts at:      0     983    1490    2499 
   Size:        983     507    1009

  Fragments arranged by size: 

                1009     983     507
 
BanI G'GyrC_C

Cuts at:      0    1907    2499 
   Size:       1907     592
 
BanII G_rGCy'C

Cuts at:      0     335     923    1264    1295    1852    1861    2035    2325 
   Size:        335     588     341      31     557       9     174     290

Cuts at:   2325    2499 
   Size:        174

  Fragments arranged by size: 

                 588     557     341     335     290     174     174      31
                   9
 
BbvCI CC'TCA_GC

Cuts at:      0    2466    2499 
   Size:       2466      33
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     565     831    2499 
   Size:        565     266    1668

  Fragments arranged by size: 

                1668     565     266
 
BlpI GC'TnA_GC

Cuts at:      0    1258    2499 
   Size:       1258    1241
 
Bme1580I G_kGCm'C

Cuts at:      0     656    1295    1455    1861    2499 
   Size:        656     639     160     406     638

  Fragments arranged by size: 

                 656     639     638     406     160
 
BmgBI CAC'GTC

Cuts at:      0    1149    2499 
   Size:       1149    1350
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2206    2499 
   Size:       2206     293
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     280     319     349     954    2419    2499 
   Size:        280      39      30     605    1465      80

  Fragments arranged by size: 

                1465     605     280      80      39      30
 
Bpu10I CC'TnA_GC

Cuts at:      0    2466    2499 
   Size:       2466      33
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      26    1036    1079    1717    2351    2499 
   Size:         26    1010      43     638     634     148

  Fragments arranged by size: 

                1010     638     634     148      43      26
 
BsaAI yAC'GTr

Cuts at:      0      80    1628    2499 
   Size:         80    1548     871

  Fragments arranged by size: 

                1548     871      80
 
BsaHI Gr'CG_yC

Cuts at:      0       5     504    1885    1963    2499 
   Size:          5     499    1381      78     536

  Fragments arranged by size: 

                1381     536     499      78       5
 
BsaWI w'CCGG_w

Cuts at:      0     540    1277    1487    1577    2499 
   Size:        540     737     210      90     922

  Fragments arranged by size: 

                 922     737     540     210      90
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1167    1197    1369    1399    2300    2330    2499 
   Size:       1167      30     172      30     901      30     169

  Fragments arranged by size: 

                1167     901     172     169      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     931    1070    1172    2425    2428    2499 
   Size:        931     139     102    1253       3      71

  Fragments arranged by size: 

                1253     931     139     102      71       3
 
BseYI C'CCAG_C

Cuts at:      0      66     442     787     824    1053    2097    2482    2499 
   Size:         66     376     345      37     229    1044     385      17

  Fragments arranged by size: 

                1044     385     376     345     229      66      37      17
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2152    2332    2424    2499 
   Size:       2152     180      92      75

  Fragments arranged by size: 

                2152     180      92      75
 
BsiEI CG_ry'CG

Cuts at:      0    1505    2044    2499 
   Size:       1505     539     455

  Fragments arranged by size: 

                1505     539     455
 
BsiHKAI G_wGCw'C

Cuts at:      0     335     365     656    1852    2060    2113    2120    2499 
   Size:        335      30     291    1196     208      53       7     379

  Fragments arranged by size: 

                1196     379     335     291     208      53      30       7
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1876    2499 
   Size:       1876     623
 
Bsp1286I G_dGCh'C

Cuts at:      0     335     365     656     923    1264    1295    1455    1852 
   Size:        335      30     291     267     341      31     160     397

Cuts at:   1852    1861    2035    2060    2113    2120    2325    2499 
   Size:          9     174      25      53       7     205     174

  Fragments arranged by size: 

                 397     341     335     291     267     205     174     174
                 160      53      31      30      25       9       7
 
BspEI T'CCGG_A

Cuts at:      0     540    1487    2499 
   Size:        540     947    1012

  Fragments arranged by size: 

                1012     947     540
 
BspHI T'CATG_A

Cuts at:      0     677    2499 
   Size:        677    1822
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      62    1547    1932    2124    2304    2499 
   Size:         62    1485     385     192     180     195

  Fragments arranged by size: 

                1485     385     195     192     180      62
 
BsrBI CCG'CTC

Cuts at:      0     642     968    1066    2499 
   Size:        642     326      98    1433

  Fragments arranged by size: 

                1433     642     326      98
 
BsrFI r'CCGG_y

Cuts at:      0     206    2499 
   Size:        206    2293
 
BssHII G'CGCG_C

Cuts at:      0    2081    2499 
   Size:       2081     418
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2310    2499 
   Size:       2310     189
 
BstEII G'GTnAC_C

Cuts at:      0    2136    2193    2499 
   Size:       2136      57     306

  Fragments arranged by size: 

                2136     306      57
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     222     621     978    2499 
   Size:        222     399     357    1521

  Fragments arranged by size: 

                1521     399     357     222
 
BstYI r'GATC_y

Cuts at:      0     263     855     955     983    1490    1799    2237    2499 
   Size:        263     592     100      28     507     309     438     262

  Fragments arranged by size: 

                 592     507     438     309     263     262     100      28
            
 
Bsu36I CC'TnA_GG

Cuts at:      0    1090    2499 
   Size:       1090    1409
 
BtgI C'CryG_G

Cuts at:      0     231    2264    2499 
   Size:        231    2033     235

  Fragments arranged by size: 

                2033     235     231
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1728    2499 
   Size:       1728     771
 
BtsI GCAGTG_nn'

Cuts at:      0    1954    2412    2424    2499 
   Size:       1954     458      12      75

  Fragments arranged by size: 

                1954     458      75      12
 
DraIII CAC_nnn'GTG

Cuts at:      0    2070    2499 
   Size:       2070     429
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1968    2499 
   Size:       1968     531
 
EaeI y'GGCC_r

Cuts at:      0     497     611     623    1502    2041    2499 
   Size:        497     114      12     879     539     458

  Fragments arranged by size: 

                 879     539     497     458     114      12
 
EagI C'GGCC_G

Cuts at:      0    1502    2041    2499 
   Size:       1502     539     458

  Fragments arranged by size: 

                1502     539     458
 
EarI CTCTTCn'nnn_

Cuts at:      0     676    1432    2065    2499 
   Size:        676     756     633     434

  Fragments arranged by size: 

                 756     676     633     434
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     963    1852    2499 
   Size:        963     889     647

  Fragments arranged by size: 

                 963     889     647
 
EcoICRI GAG'CTC

Cuts at:      0     333    1850    2499 
   Size:        333    1517     649

  Fragments arranged by size: 

                1517     649     333
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     160     280     319     349     583     730     892 
   Size:        154       6     120      39      30     234     147     162

Cuts at:    892     954    1885    2272    2419    2499 
   Size:         62     931     387     147      80

  Fragments arranged by size: 

                 931     387     234     162     154     147     147     120
                  80      62      39      30       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     178    2499 
   Size:        178    2321
 
EcoO109I rG'GnC_Cy

Cuts at:      0     120     889    1228    1292    1857    2125    2207    2499 
   Size:        120     769     339      64     565     268      82     292

  Fragments arranged by size: 

                 769     565     339     292     268     120      82      64
            
 
EcoRI G'AATT_C

Cuts at:      0     274    2499 
   Size:        274    2225
 
HaeII r_GCGC'y

Cuts at:      0     794    2499 
   Size:        794    1705
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      97     129     151     183     410     442     580     612 
   Size:         97      32      22      32     227      32     138      32

Cuts at:    612     756     788     792     824     833     840     865     872 
   Size:        144      32       4      32       9       7      25       7

Cuts at:    872    2406    2438    2499 
   Size:       1534      32      61

  Fragments arranged by size: 

                1534     227     144     138      97      61      32      32
                  32      32      32      32      32      25      22       9
                   7       7       4
 
KpnI G_GTAC'C

Cuts at:      0    1911    2499 
   Size:       1911     588
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2256    2499 
   Size:       2256     243
 
MscI TGG'CCA

Cuts at:      0     499    2499 
   Size:        499    2000
 
MslI CAynn'nnrTG

Cuts at:      0     799    2026    2499 
   Size:        799    1227     473

  Fragments arranged by size: 

                1227     799     473
 
MspA1I CmG'CkG

Cuts at:      0      63     553    1257    2014    2340    2499 
   Size:         63     490     704     757     326     159

  Fragments arranged by size: 

                 757     704     490     326     159      63
 
NaeI GCC'GGC

Cuts at:      0     208    2499 
   Size:        208    2291
 
NcoI C'CATG_G

Cuts at:      0     231    2499 
   Size:        231    2268
 
NgoMIV G'CCGG_C

Cuts at:      0     206    2499 
   Size:        206    2293
 
PasI CC'CwG_GG

Cuts at:      0     600    2499 
   Size:        600    1899
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     372    2499 
   Size:        372    2127
 
PfoI T'CCnGG_A

Cuts at:      0     296     851    1802    2499 
   Size:        296     555     951     697

  Fragments arranged by size: 

                 951     697     555     296
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1355    1369    1387    1401    2499 
   Size:       1355      14      18      14    1098

  Fragments arranged by size: 

                1355    1098      18      14      14
 
PpuMI rG'GwC_Cy

Cuts at:      0     120     889    1228    2125    2207    2499 
   Size:        120     769     339     897      82     292

  Fragments arranged by size: 

                 897     769     339     292     120      82
 
PshAI GACnn'nnGTC

Cuts at:      0    1001    1226    2499 
   Size:       1001     225    1273

  Fragments arranged by size: 

                1273    1001     225
 
PspOMI G'GGCC_C

Cuts at:      0    1291    1857    2499 
   Size:       1291     566     642

  Fragments arranged by size: 

                1291     642     566
 
PstI C_TGCA'G

Cuts at:      0    1170    2499 
   Size:       1170    1329
 
PvuII CAG'CTG

Cuts at:      0     553    1257    2340    2499 
   Size:        553     704    1083     159

  Fragments arranged by size: 

                1083     704     553     159
 
SacI G_AGCT'C

Cuts at:      0     335    1852    2499 
   Size:        335    1517     647

  Fragments arranged by size: 

                1517     647     335
 
SanDI GG'GwC_CC

Cuts at:      0    2125    2207    2499 
   Size:       2125      82     292

  Fragments arranged by size: 

                2125     292      82
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2065    2499 
   Size:       2065     434
 
SexAI A'CCwGG_T

Cuts at:      0     122    2499 
   Size:        122    2377
 
SfcI C'TryA_G

Cuts at:      0      58     631    1072    1166    2344    2499 
   Size:         58     573     441      94    1178     155

  Fragments arranged by size: 

                1178     573     441     155      94      58
 
SmaI CCC'GGG

Cuts at:      0      40     882    1265    1390    2037    2499 
   Size:         40     842     383     125     647     462

  Fragments arranged by size: 

                 842     647     462     383     125      40
 
SmlI C'TyrA_G

Cuts at:      0     101    1082    2499 
   Size:        101     981    1417

  Fragments arranged by size: 

                1417     981     101
 
StuI AGG'CCT

Cuts at:      0     845    2293    2495    2499 
   Size:        845    1448     202       4

  Fragments arranged by size: 

                1448     845     202       4
 
StyI C'CwwG_G

Cuts at:      0     231    1526    1701    2499 
   Size:        231    1295     175     798

  Fragments arranged by size: 

                1295     798     231     175
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      58     976    1620    2263    2499 
   Size:         58     918     644     643     236

  Fragments arranged by size: 

                 918     644     643     236      58
 
TatI w'GTAC_w

Cuts at:      0    1634    2499 
   Size:       1634     865
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1167    1199    2222    2254    2300    2332    2499 
   Size:       1167      32    1023      32      46      32     167

  Fragments arranged by size: 

                1167    1023     167      46      32      32      32
 
XhoI C'TCGA_G

Cuts at:      0     101    1082    2499 
   Size:        101     981    1417

  Fragments arranged by size: 

                1417     981     101
 
XmaI C'CCGG_G

Cuts at:      0      38     880    1263    1388    2035    2499 
   Size:         38     842     383     125     647     464

  Fragments arranged by size: 

                 842     647     464     383     125      38
 
XmnI GAAnn'nnTTC

Cuts at:      0    1924    2499 
   Size:       1924     575
 
ZraI GAC'GTC

Cuts at:      0       6    1886    2499 
   Size:          6    1880     613

  Fragments arranged by size: 

                1880     613       6
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AclI     AcuI     AhdI     AloI    AlwNI
     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII    BbvCI
    BciVI     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI
   BssHII   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII    Hin4I     KpnI     MmeI
     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     PasI    PflMI
     PfoI     PpiI    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI
    SanDI     SapI    SexAI     SfcI     SmaI     SmlI     StuI     StyI
    TaqII     TatI     TstI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AccI     AfeI    AflII   AflIII     AgeI     AleI     AlfI     AscI
     AseI    AsiSI    AvrII     BaeI     BbeI     BbsI     BcgI     BclI
    BfrBI     BglI    BglII     BmtI     BplI    BpuEI    BsaBI    BsiWI
     BsmI    BsrDI    BsrGI    BssSI    BstBI  BstZ17I     ClaI    CspCI
     DraI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     MfeI     MluI     NarI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI     PmeI     PmlI     PsiI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI  Tth111I     XbaI     XcmI
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