Restriction map for pF1KA0705
 (Linear) (Six-base) MAPSORT of: KIAA0705.seq  Check: 6483  from: 1  to: 4323



KIAA0705.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     772    1341    2169    4323 
   Size:        772     569     828    2154

  Fragments arranged by size: 

                2154     828     772     569
 
AccI GT'mk_AC

Cuts at:      0     953    4323 
   Size:        953    3370
 
Acc65I G'GTAC_C

Cuts at:      0     550    4323 
   Size:        550    3773
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     358     653     868     905    1568    2310    2702 
   Size:        115     243     295     215      37     663     742     392

Cuts at:   2702    3121    4323 
   Size:        419    1202

  Fragments arranged by size: 

                1202     742     663     419     392     295     243     215
                 115      37
 
AfeI AGC'GCT

Cuts at:      0    3904    4323 
   Size:       3904     419
 
AflII C'TTAA_G

Cuts at:      0    1996    4323 
   Size:       1996    2327
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1770    4323 
   Size:       1770    2553
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1968    2148    2590    4323 
   Size:       1968     180     442    1733

  Fragments arranged by size: 

                1968    1733     442     180
 
ApaI G_GGCC'C

Cuts at:      0    4204    4256    4323 
   Size:       4204      52      67

  Fragments arranged by size: 

                4204      67      52
 
AseI AT'TA_AT

Cuts at:      0    1427    4323 
   Size:       1427    2896
 
AvaI C'yCGr_G

Cuts at:      0    1241    2335    3886    3940    3970    4006    4104    4131 
   Size:       1241    1094    1551      54      30      36      98      27

Cuts at:   4131    4212    4323 
   Size:         81     111

  Fragments arranged by size: 

                1551    1241    1094     111      98      81      54      36
                  30      27
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     534     567    4323 
   Size:        534      33    3756

  Fragments arranged by size: 

                3756     534      33
 
BamHI G'GATC_C

Cuts at:      0     993    2791    2879    4323 
   Size:        993    1798      88    1444

  Fragments arranged by size: 

                1798    1444     993      88
 
BanI G'GyrC_C

Cuts at:      0     103     411     550     784    1569    1831    3009    3948 
   Size:        103     308     139     234     785     262    1178     939

Cuts at:   3948    4002    4059    4219    4263    4303    4323 
   Size:         54      57     160      44      40      20

  Fragments arranged by size: 

                1178     939     785     308     262     234     160     139
                 103      57      54      44      40      20
 
BanII G_rGCy'C

Cuts at:      0     230    2179    2234    2392    2561    2993    4013    4081 
   Size:        230    1949      55     158     169     432    1020      68

Cuts at:   4081    4088    4204    4256    4323 
   Size:          7     116      52      67

  Fragments arranged by size: 

                1949    1020     432     230     169     158     116      68
                  67      55      52       7
 
BbeI G_GCGC'C

Cuts at:      0     107    3952    4006    4063    4223    4307    4323 
   Size:        107    3845      54      57     160      84      16

  Fragments arranged by size: 

                3845     160     107      84      57      54      16
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     542     776     893    1556    1626    3618    4323 
   Size:        542     234     117     663      70    1992     705

  Fragments arranged by size: 

                1992     705     663     542     234     117      70
 
BbvCI CC'TCA_GC

Cuts at:      0    1273    4323 
   Size:       1273    3050
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     367     401    4323 
   Size:        367      34    3922

  Fragments arranged by size: 

                3922     367      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1754    2604    4323 
   Size:       1754     850    1719

  Fragments arranged by size: 

                1754    1719     850
 
BclI T'GATC_A

Cuts at:      0     254    4323 
   Size:        254    4069
 
BfrBI ATG'CAT

Cuts at:      0    1249    4323 
   Size:       1249    3074
 
BglI GCCn_nnn'nGGC

Cuts at:      0      83    3994    4136    4323 
   Size:         83    3911     142     187

  Fragments arranged by size: 

                3911     187     142      83
 
BlpI GC'TnA_GC

Cuts at:      0    2391    4323 
   Size:       2391    1932
 
Bme1580I G_kGCm'C

Cuts at:      0    1836    2810    4204    4256    4323 
   Size:       1836     974    1394      52      67

  Fragments arranged by size: 

                1836    1394     974      67      52
 
BmgBI CAC'GTC

Cuts at:      0     243    4323 
   Size:        243    4080
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     930    1248    2654    4323 
   Size:        930     318    1406    1669

  Fragments arranged by size: 

                1669    1406     930     318
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     819    1616    4323 
   Size:        819     797    2707

  Fragments arranged by size: 

                2707     819     797
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     473    1007    1586    3626    3699    4177    4323 
   Size:        220     253     534     579    2040      73     478     146

  Fragments arranged by size: 

                2040     579     534     478     253     220     146      73
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1273    1969    4323 
   Size:       1273     696    2354

  Fragments arranged by size: 

                2354    1273     696
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268    3616    4323 
   Size:        268    3348     707

  Fragments arranged by size: 

                3348     707     268
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     205    2867    4323 
   Size:        205    2662    1456

  Fragments arranged by size: 

                2662    1456     205
 
BsaAI yAC'GTr

Cuts at:      0    1742    4323 
   Size:       1742    2581
 
BsaBI GATnn'nnATC

Cuts at:      0    2294    4323 
   Size:       2294    2029
 
BsaHI Gr'CG_yC

Cuts at:      0     104    2268    3722    3949    4003    4060    4220    4304 
   Size:        104    2164    1454     227      54      57     160      84

Cuts at:   4304    4323 
   Size:         19

  Fragments arranged by size: 

                2164    1454     227     160     104      84      57      54
                  19
 
BsaWI w'CCGG_w

Cuts at:      0    1368    2876    4323 
   Size:       1368    1508    1447

  Fragments arranged by size: 

                1508    1447    1368
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     293     323     728     758    2161    2191    4323 
   Size:        293      30     405      30    1403      30    2132

  Fragments arranged by size: 

                2132    1403     405     293      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     202     712     856    2057    3001    3958    4323 
   Size:        202     510     144    1201     944     957     365

  Fragments arranged by size: 

                1201     957     944     510     365     202     144
 
BseYI C'CCAG_C

Cuts at:      0      81     806    1058    3013    3077    3087    3113    4285 
   Size:         81     725     252    1955      64      10      26    1172

Cuts at:   4285    4323 
   Size:         38

  Fragments arranged by size: 

                1955    1172     725     252      81      64      38      26
                  10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     800     803    1690    4323 
   Size:        800       3     887    2633

  Fragments arranged by size: 

                2633     887     800       3
 
BsiEI CG_ry'CG

Cuts at:      0    1693    3934    4198    4313    4323 
   Size:       1693    2241     264     115      10

  Fragments arranged by size: 

                2241    1693     264     115      10
 
BsiHKAI G_wGCw'C

Cuts at:      0     513    2179    2234    2993    4088    4323 
   Size:        513    1666      55     759    1095     235

  Fragments arranged by size: 

                1666    1095     759     513     235      55
 
BsmI GAATG_Cn'

Cuts at:      0    3437    4323 
   Size:       3437     886
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1322    3729    4323 
   Size:       1322    2407     594

  Fragments arranged by size: 

                2407    1322     594
 
Bsp1286I G_dGCh'C

Cuts at:      0     230     513    1836    2179    2234    2392    2561    2810 
   Size:        230     283    1323     343      55     158     169     249

Cuts at:   2810    2993    4013    4081    4088    4204    4256    4323 
   Size:        183    1020      68       7     116      52      67

  Fragments arranged by size: 

                1323    1020     343     283     249     230     183     169
                 158     116      68      67      55      52       7
 
BspEI T'CCGG_A

Cuts at:      0    1368    4323 
   Size:       1368    2955
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     772    1341    2156    2169    2505    2941    4323 
   Size:        772     569     815      13     336     436    1382

  Fragments arranged by size: 

                1382     815     772     569     436     336      13
 
BsrBI CCG'CTC

Cuts at:      0     167    4323 
   Size:        167    4156
 
BsrDI GCAATG_nn'

Cuts at:      0     907    2378    2514    2673    4323 
   Size:        907    1471     136     159    1650

  Fragments arranged by size: 

                1650    1471     907     159     136
 
BsrFI r'CCGG_y

Cuts at:      0    2458    3976    4091    4116    4126    4323 
   Size:       2458    1518     115      25      10     197

  Fragments arranged by size: 

                2458    1518     197     115      25      10
 
BsrGI T'GTAC_A

Cuts at:      0     827    1959    4323 
   Size:        827    1132    2364

  Fragments arranged by size: 

                2364    1132     827
 
BssHII G'CGCG_C

Cuts at:      0    4151    4180    4182    4228    4323 
   Size:       4151      29       2      46      95

  Fragments arranged by size: 

                4151      95      46      29       2
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1713    2148    4323 
   Size:       1713     435    2175

  Fragments arranged by size: 

                2175    1713     435
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1058    2718    4323 
   Size:       1058    1660    1605

  Fragments arranged by size: 

                1660    1605    1058
 
BstYI r'GATC_y

Cuts at:      0     993    2791    2879    4323 
   Size:        993    1798      88    1444

  Fragments arranged by size: 

                1798    1444     993      88
 
Bsu36I CC'TnA_GG

Cuts at:      0     695     789     839    2981    4323 
   Size:        695      94      50    2142    1342

  Fragments arranged by size: 

                2142    1342     695      94      50
 
BtgI C'CryG_G

Cuts at:      0     218    2059    2209    2384    3035    4323 
   Size:        218    1841     150     175     651    1288

  Fragments arranged by size: 

                1841    1288     651     218     175     150
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3093    4323 
   Size:       3093    1230
 
BtsI GCAGTG_nn'

Cuts at:      0     261    2625    2894    4323 
   Size:        261    2364     269    1429

  Fragments arranged by size: 

                2364    1429     269     261
 
ClaI AT'CG_AT

Cuts at:      0    1070    4323 
   Size:       1070    3253
 
DraIII CAC_nnn'GTG

Cuts at:      0    2620    4323 
   Size:       2620    1703
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1665    4323 
   Size:       1665    2658
 
EaeI y'GGCC_r

Cuts at:      0     248    1690    2132    2406    2895    3867    3931    3957 
   Size:        248    1442     442     274     489     972      64      26

Cuts at:   3957    4195    4310    4323 
   Size:        238     115      13

  Fragments arranged by size: 

                1442     972     489     442     274     248     238     115
                  64      26      13
 
EagI C'GGCC_G

Cuts at:      0    1690    3931    4195    4310    4323 
   Size:       1690    2241     264     115      13

  Fragments arranged by size: 

                2241    1690     264     115      13
 
EarI CTCTTCn'nnn_

Cuts at:      0     640     698     704    1022    1240    4323 
   Size:        640      58       6     318     218    3083

  Fragments arranged by size: 

                3083     640     318     218      58       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    4045    4323 
   Size:       4045     278
 
EcoICRI GAG'CTC

Cuts at:      0    2177    2232    2991    4086    4323 
   Size:       2177      55     759    1095     237

  Fragments arranged by size: 

                2177    1095     759     237      55
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     220     358     473     653     868     905    1007 
   Size:        115     105     138     115     180     215      37     102

Cuts at:   1007    1568    1586    2310    2702    3121    3626    3699    4177 
   Size:        561      18     724     392     419     505      73     478

Cuts at:   4177    4323 
   Size:        146

  Fragments arranged by size: 

                 724     561     505     478     419     392     215     180
                 146     138     115     115     105     102      73      37
                  18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     133    4323 
   Size:        133    4190
 
EcoO109I rG'GnC_Cy

Cuts at:      0     270     447    1209    3651    4189    4252    4253    4323 
   Size:        270     177     762    2442     538      63       1      70

  Fragments arranged by size: 

                2442     762     538     270     177      70      63       1
            
 
EcoRV GAT'ATC

Cuts at:      0    3819    4323 
   Size:       3819     504
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     545    1822    2039    4323 
   Size:        545    1277     217    2284

  Fragments arranged by size: 

                2284    1277     545     217
 
HaeII r_GCGC'y

Cuts at:      0     107    3885    3906    3952    4006    4063    4223    4307 
   Size:        107    3778      21      46      54      57     160      84

Cuts at:   4307    4323 
   Size:         16

  Fragments arranged by size: 

                3778     160     107      84      57      54      46      21
                  16
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1301    1333    1756    1788    2022    2054    2776    2808 
   Size:       1301      32     423      32     234      32     722      32

Cuts at:   2808    2864    2896    3521    3553    4323 
   Size:         56      32     625      32     770

  Fragments arranged by size: 

                1301     770     722     625     423     234      56      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1101    1506    2520    4323 
   Size:       1101     405    1014    1803

  Fragments arranged by size: 

                1803    1101    1014     405
 
HindIII A'AGCT_T

Cuts at:      0       9    4323 
   Size:          9    4314
 
KasI G'GCGC_C

Cuts at:      0     103    3948    4002    4059    4219    4303    4323 
   Size:        103    3845      54      57     160      84      20

  Fragments arranged by size: 

                3845     160     103      84      57      54      20
 
KpnI G_GTAC'C

Cuts at:      0     554    4323 
   Size:        554    3769
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     164    3231    3341    3803    4323 
   Size:        164    3067     110     462     520

  Fragments arranged by size: 

                3067     520     462     164     110
 
MscI TGG'CCA

Cuts at:      0    2897    4323 
   Size:       2897    1426
 
MslI CAynn'nnrTG

Cuts at:      0      50    1454    1460    1628    2383    2474    2911    4323 
   Size:         50    1404       6     168     755      91     437    1412

  Fragments arranged by size: 

                1412    1404     755     437     168      91      50       6
            
 
MspA1I CmG'CkG

Cuts at:      0      81    1878    3141    3243    3691    4148    4323 
   Size:         81    1797    1263     102     448     457     175

  Fragments arranged by size: 

                1797    1263     457     448     175     102      81
 
NaeI GCC'GGC

Cuts at:      0    2460    3978    4093    4118    4128    4323 
   Size:       2460    1518     115      25      10     195

  Fragments arranged by size: 

                2460    1518     195     115      25      10
 
NarI GG'CG_CC

Cuts at:      0     104    3949    4003    4060    4220    4304    4323 
   Size:        104    3845      54      57     160      84      19

  Fragments arranged by size: 

                3845     160     104      84      57      54      19
 
NcoI C'CATG_G

Cuts at:      0    2059    2384    3035    4323 
   Size:       2059     325     651    1288

  Fragments arranged by size: 

                2059    1288     651     325
 
NdeI CA'TA_TG

Cuts at:      0    1053    2226    4323 
   Size:       1053    1173    2097

  Fragments arranged by size: 

                2097    1173    1053
 
NgoMIV G'CCGG_C

Cuts at:      0    2458    3976    4091    4116    4126    4323 
   Size:       2458    1518     115      25      10     197

  Fragments arranged by size: 

                2458    1518     197     115      25      10
 
NsiI A_TGCA'T

Cuts at:      0    1251    4323 
   Size:       1251    3072
 
NspI r_CATG'y

Cuts at:      0    1176    2916    3580    4323 
   Size:       1176    1740     664     743

  Fragments arranged by size: 

                1740    1176     743     664
 
PasI CC'CwG_GG

Cuts at:      0    1836    4323 
   Size:       1836    2487
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1723    4323 
   Size:       1723    2600
 
PfoI T'CCnGG_A

Cuts at:      0     612    1139    4323 
   Size:        612     527    3184

  Fragments arranged by size: 

                3184     612     527
 
PpuMI rG'GwC_Cy

Cuts at:      0     270     447    3651    4189    4323 
   Size:        270     177    3204     538     134

  Fragments arranged by size: 

                3204     538     270     177     134
 
PshAI GACnn'nnGTC

Cuts at:      0     323    4323 
   Size:        323    4000
 
PsiI TTA'TAA

Cuts at:      0    2279    4323 
   Size:       2279    2044
 
PspOMI G'GGCC_C

Cuts at:      0    4200    4252    4323 
   Size:       4200      52      71

  Fragments arranged by size: 

                4200      71      52
 
PspXI vC'TCGA_Gb

Cuts at:      0    3940    4323 
   Size:       3940     383
 
PstI C_TGCA'G

Cuts at:      0    1208    1352    1383    2708    2847    4323 
   Size:       1208     144      31    1325     139    1476

  Fragments arranged by size: 

                1476    1325    1208     144     139      31
 
PvuII CAG'CTG

Cuts at:      0      81    3141    4323 
   Size:         81    3060    1182

  Fragments arranged by size: 

                3060    1182      81
 
SacI G_AGCT'C

Cuts at:      0    2179    2234    2993    4088    4323 
   Size:       2179      55     759    1095     235

  Fragments arranged by size: 

                2179    1095     759     235      55
 
SanDI GG'GwC_CC

Cuts at:      0    4189    4323 
   Size:       4189     134
 
SbfI CC_TGCA'GG

Cuts at:      0    1208    4323 
   Size:       1208    3115
 
SexAI A'CCwGG_T

Cuts at:      0    1508    4323 
   Size:       1508    2815
 
SfcI C'TryA_G

Cuts at:      0    1159    1204    1348    1379    1868    2704    2843    3228 
   Size:       1159      45     144      31     489     836     139     385

Cuts at:   3228    3297    3321    3366    4323 
   Size:         69      24      45     957

  Fragments arranged by size: 

                1159     957     836     489     385     144     139      69
                  45      45      31      24
 
SfoI GGC'GCC

Cuts at:      0     105    3950    4004    4061    4221    4305    4323 
   Size:        105    3845      54      57     160      84      18

  Fragments arranged by size: 

                3845     160     105      84      57      54      18
 
SgrAI Cr'CCGG_yG

Cuts at:      0    4116    4323 
   Size:       4116     207
 
SmaI CCC'GGG

Cuts at:      0    1243    4008    4106    4133    4214    4323 
   Size:       1243    2765      98      27      81     109

  Fragments arranged by size: 

                2765    1243     109      98      81      27
 
SmlI C'TyrA_G

Cuts at:      0     283    1996    3631    3940    4323 
   Size:        283    1713    1635     309     383

  Fragments arranged by size: 

                1713    1635     383     309     283
 
SrfI GCCC'GGGC

Cuts at:      0    4008    4133    4323 
   Size:       4008     125     190

  Fragments arranged by size: 

                4008     190     125
 
StuI AGG'CCT

Cuts at:      0     717    2200    2781    4323 
   Size:        717    1483     581    1542

  Fragments arranged by size: 

                1542    1483     717     581
 
StyI C'CwwG_G

Cuts at:      0    1443    2059    2384    3035    3290    4323 
   Size:       1443     616     325     651     255    1033

  Fragments arranged by size: 

                1443    1033     651     616     325     255
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1810    2102    2432    2614    3027    4323 
   Size:       1810     292     330     182     413    1296

  Fragments arranged by size: 

                1810    1296     413     330     292     182
 
TatI w'GTAC_w

Cuts at:      0     827    1646    1959    4323 
   Size:        827     819     313    2364

  Fragments arranged by size: 

                2364     827     819     313
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1739    1771    1865    1897    2161    2193    3602    3634 
   Size:       1739      32      94      32     264      32    1409      32

Cuts at:   3634    4323 
   Size:        689

  Fragments arranged by size: 

                1739    1409     689     264      94      32      32      32
                  32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1186    3809    4323 
   Size:       1186    2623     514

  Fragments arranged by size: 

                2623    1186     514
 
XhoI C'TCGA_G

Cuts at:      0    3940    4323 
   Size:       3940     383
 
XmaI C'CCGG_G

Cuts at:      0    1241    4006    4104    4131    4212    4323 
   Size:       1241    2765      98      27      81     111

  Fragments arranged by size: 

                2765    1241     111      98      81      27
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI    AflII     AhdI    AlwNI
     ApaI     AseI     AvaI     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI
    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NsiI
     NspI     PasI    PflMI     PfoI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PstI    PvuII     SacI    SanDI     SbfI    SexAI     SfcI
     SfoI    SgrAI     SmaI     SmlI     SrfI     StuI     StyI    TaqII
     TatI     TstI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AclI   AflIII     AgeI     AleI     AlfI     AloI    ApaLI
     ApoI     AscI    AsiSI    AvrII    BglII     BmtI    BsiWI    BspHI
    BssSI    BstBI   BstEII  BstZ17I    CspCI     DraI    EcoRI     FseI
     FspI    FspAI     HpaI     MfeI     MluI     NheI     NotI     NruI
     PacI     PciI     PmeI     PmlI     PpiI     PsrI     PvuI    RsrII
    SacII     SalI     SapI     ScaI     SfiI     SgfI    SnaBI     SpeI
     SphI     SspI     SwaI  Tth111I     XbaI     XmnI     ZraI
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